Lipidomics profiling of zebrafish liver through untargeted liquid chromatography‐high resolution mass spectrometry

Silva, Katyeny Manuela da; Iturrospe, Elias; Boom, Rik van den; Lavoir, Maria van de; Robeyns, Rani; Vergauwen, Lucia; Knapen, Dries; Cuykx, Matthias; Nuijs, Alexander L.N. van

doi:10.1002/jssc.202200214

Cited by 8 publications

(3 citation statements)

References 51 publications

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“…Lipidomic analysis can also be applied to specific organs from adult zebrafish. For instance, livers from 6-month-old animals were analyzed with different MS techniques, identifying 712 unique lipid species from four categories (fatty acyls, glycerolipids, glycerophospholipids, and sphingolipids) [109]. Moreover, adult zebrafish brains have been analyzed for changes in the lipid profile after exposure to different xenobiotics [102,110].…”

Section: Zebrafish Lipidomicsmentioning

confidence: 99%

Zebra-Sphinx: Modeling Sphingolipidoses in Zebrafish

Mignani

Guerra

Corli

et al. 2023

IJMS

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Sphingolipidoses are inborn errors of metabolism due to the pathogenic mutation of genes that encode for lysosomal enzymes, transporters, or enzyme cofactors that participate in the sphingolipid catabolism. They represent a subgroup of lysosomal storage diseases characterized by the gradual lysosomal accumulation of the substrate(s) of the defective proteins. The clinical presentation of patients affected by sphingolipid storage disorders ranges from a mild progression for some juvenile- or adult-onset forms to severe/fatal infantile forms. Despite significant therapeutic achievements, novel strategies are required at basic, clinical, and translational levels to improve patient outcomes. On these bases, the development of in vivo models is crucial for a better understanding of the pathogenesis of sphingolipidoses and for the development of efficacious therapeutic strategies. The teleost zebrafish (Danio rerio) has emerged as a useful platform to model several human genetic diseases owing to the high grade of genome conservation between human and zebrafish, combined with precise genome editing and the ease of manipulation. In addition, lipidomic studies have allowed the identification in zebrafish of all of the main classes of lipids present in mammals, supporting the possibility to model diseases of the lipidic metabolism in this animal species with the advantage of using mammalian lipid databases for data processing. This review highlights the use of zebrafish as an innovative model system to gain novel insights into the pathogenesis of sphingolipidoses, with possible implications for the identification of more efficacious therapeutic approaches.

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Section: Zebrafish Lipidomicsmentioning

confidence: 99%

Zebra-Sphinx: Modeling Sphingolipidoses in Zebrafish

Mignani

Guerra

Corli

et al. 2023

IJMS

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“…A recent review of bioinformatics tools for MS-based lipidomics analysis revealed over 30 open-access software tools for lipidomics data processing and annotation 16 . MS-Dial, for instance, is one of the most 6 used software tools due to its versatility and free access, making it widely adopted in the lipidomics field [16][17][18] . Some annotation tools, such as Lipid Annotator, are not freely accessible, limiting their usage compared to other more widely available tools.…”

Section: Comprehensive Workflow For An Accurate Lipid Annotation Usin...mentioning

confidence: 99%

LiLA: Lipid Lung-based ATLAS built Through a Comprehensive Workflow Designed for an Accurate Lipid Annotation

Gonzalez-Riano,

Requena,

Nadeem

et al. 2023

Preprint

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Accurate lipid annotation is crucial for understanding the role of lipids in health and disease and identifying therapeutic targets. However, annotating the wide variety of lipid species in biological samples remains challenging in untargeted lipidomic studies. In this work, we present an optimized lipid annotation workflow based on the combination of LC-MS and MS/MS strategies, four bioinformatic tools, and a decision-tree-based approach to support the accurate annotation and semi-quantification of the lipid species present in lung tissue from control mice. The developed workflow allowed us to generate a lipid lung-based ATLAS (LiLA), which was then employed to unveil the lipidomic signatures of the Mycobacterium tuberculosis (Mtb) infection at two different time points for a deeper understanding of the disease progression. This workflow, combined with manual inspection strategies of MS/MS data, can enhance the annotation process for lipidomic studies and guide the generation of sample-specific lipidome maps. LiLA serves as a freely available data resource that can be employed in future studies to address lipidomic alterations in mice lung tissue.

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“…Driven by the biological significance of lipids, lipidomics has developed as an emerging omics science. In recent years, MS, especially hyphenated with ultrahigh performance liquid chromatography, enables rapid separation and sensitive detection of hundreds of lipids [18,19].…”

Section: Introductionmentioning

confidence: 99%

Ultra‐high performance liquid chromatography‐quadrupole/time‐of‐flight mass spectrometry‐based lipidomics reveals key lipid molecules as potential therapeutic targets of Polygonum cuspidatum against hyperlipidemia in a hamster model

Yang

et al. 2023

J of Separation Science

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Polygonum cuspidatum is a homology of traditional medicine and functional food widely distributed around the world. Our previous study on the hyperlipidemic animal model demonstrated that Polygonum cuspidatum was effective in ameliorating hyperlipidemia, which is characterized by lipid disorders. Herein, the regulatory effect of Polygonum cuspidatum on lipid metabolism needs to be known if its hypolipidemic mechanism is desired to clarify. In this study, an ultra-high performance liquid chromatography-quadrupole/time-of-flight mass spectrometry-based lipidomic strategy was first applied to investigate the lipidomic patterns of high-fat diet-induced hyperlipidemic hamsters when treated with Polygonum cuspidatum. The results showed that Polygonum cuspidatum improved the lipidomic profile of hyperlipidemia. A total of 65 differential lipids related to the hypolipidemic effect of Polygonum cuspidatum were screened out and identified, and these differential lipids covered various categories, such as phosphatidylcholines, phosphatidylethanolamines, triacylglycerols, sphingomyelins and so on. Orally administrated Polygonum cuspidatum restored these differential lipids back to normal or nearly normal levels. This study adopted lipidomics to reveal the key lipid molecules as potential therapeutic targets of

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Lipidomics profiling of zebrafish liver through untargeted liquid chromatography‐high resolution mass spectrometry

Cited by 8 publications

References 51 publications

Zebra-Sphinx: Modeling Sphingolipidoses in Zebrafish

Zebra-Sphinx: Modeling Sphingolipidoses in Zebrafish

LiLA: Lipid Lung-based ATLAS built Through a Comprehensive Workflow Designed for an Accurate Lipid Annotation

Ultra‐high performance liquid chromatography‐quadrupole/time‐of‐flight mass spectrometry‐based lipidomics reveals key lipid molecules as potential therapeutic targets of Polygonum cuspidatum against hyperlipidemia in a hamster model

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