Advances on the chromatographic determination of amphenicols in food

Guidi, Letícia Rocha; Tette, Patrícia Amaral Souza; Fernandes, Christian; Silva, Luiza H.M.; Glória, Maria Beatriz Abreu

doi:10.1016/j.talanta.2016.09.068

Cited by 46 publications

(20 citation statements)

References 97 publications

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“…FLO is now widely used not only for therapeutic and prophylactic purposes in veterinary medicine, but also for promoting animal growth, especially in aquaculture 1 . Moreover, residue of FLO in animal products has proved to be a global problem, a clear understanding of the mechanism of FLO-induced cytotoxicity would be benefit to the development of new drugs and to human and animal health worldwide.…”

Section: Discussionmentioning

confidence: 99%

“…Antibiotics are widely used for therapeutic and prophylactic purposes in human and veterinary medicine and also to promote growth and increase feed efficiencies in food producing animals 1 . However, abused or heavy use of antibiotics leads to development of resistance of target pathogens against antibiotics, induced allergic reactions in some hypersensitive individuals, potential compromise of the human intestinal, heart and immune systems 2 .…”

Section: Introductionmentioning

confidence: 99%

“…However, abused or heavy use of antibiotics leads to development of resistance of target pathogens against antibiotics, induced allergic reactions in some hypersensitive individuals, potential compromise of the human intestinal, heart and immune systems 2 . Florfenicol (FLO) is a broad-spectrum antibiotic widely used in veterinary medicine to against bacterial infections 3 and to promote animal growth, especially in aquaculture 1 . Previous studies have showed that FLO had side effects in multiple organs of various species 3 , 4 .…”

Section: Introductionmentioning

confidence: 99%

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Florfenicol-induced Mitochondrial Dysfunction Suppresses Cell Proliferation and Autophagy in Fibroblasts

Cao

Zhang

et al. 2017

Sci Rep

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Florfenicol (FLO) is one of the most popular antibiotics used in veterinary clinic and aquaculture. FLO can inhibit both bacterial and mitochondrial protein synthesis. However, the effects of FLO on mitochondrial function and cellular homeostasis remain unclear. Here we show that FLO inhibits expression of mitochondrial DNA-encoded proteins, decreases mitochondrial membrane potential, and promotes generation of reactive oxygen species (ROS) in vitro. As a result, activities of mitochondrial respiratory chain complex I and IV and the cellular ATP level are decreased and mitochondrial morphology is damaged. FLO represses cell growth and proliferation by suppression of phosphorylation of p70S6K through AMPK/mTOR/p70S6K pathway. Furthermore, FLO also induces G0/G1 cell cycle arrest via increase of p21 levels through activating ROS/p53/p21 pathway. Moreover, the clearance of damaged mitochondria by autophagy is impaired, leading to cell proliferation inhibition and promotes cell senescence. In addition, FLO-induced upregulation of cytosolic p53 may contribute to mitophagy deficiency via regulation of Parkin recruitment. In summary, our data suggest that florfenicol is an inhibitor of mitochondrial protein synthesis that can induce noticeable cytotoxicity. Thus, these findings can be useful for guiding the proper use of FLO and the development of safe drugs.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Florfenicol-induced Mitochondrial Dysfunction Suppresses Cell Proliferation and Autophagy in Fibroblasts

Cao

Zhang

et al. 2017

Sci Rep

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“…Traditional methods for detecting antibiotics in food include microbiological approaches that are time‐consuming and have low specificity . Among other approaches are instrumental analytical methods, such as liquid chromatography–tandem mass spectrometry and high‐performance liquid chromatography . Despite adequate accuracy, high precision and sensitivity, chromatographic methods require the use of complex equipment and highly qualified personnel.…”

Section: Introductionmentioning

confidence: 99%

“…12 Among other approaches are instrumental analytical methods, such as liquid chromatography-tandem mass spectrometry and high-performance liquid chromatography. 13,14 Despite adequate accuracy, high precision and sensitivity, chromatographic methods require the use of complex equipment and highly qualified personnel. In addition, these methods are characterized by long, complex procedures for sample preparation before analysis.…”

Section: Introductionmentioning

confidence: 99%

Development of a multicomponent immunochromatographic test system for the detection of fluoroquinolone and amphenicol antibiotics in dairy products

et al. 2019

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BACKGROUND Ciprofloxacin (CIP) and chloramphenicol (CAP) are relevant antibiotics of the fluoroquinolone (FQ) and amphenicol (AP) groups, respectively, widely used in veterinary practice and they contaminate agricultural products. In this study, a rapid and sensitive immunochromatographic assay (ICA) was developed for simultaneous detection of CIP and CAP in dairy products. The ICA was carried out in a direct competitive format using gold nanoparticles as a label. RESULTS The ICA developed here allowed for the detection of CIP and CAP in Triton X‐100‐containing buffered saline (PBST) within 15 min with instrumental detection limits of 20 pg mL−1 and 0.5 ng mL−1, respectively, and with a visual detection limit of 5 ng mL−1 for both antibiotics. The ICA showed cross‐reactivity (69–160%) to 19 antibiotics in the FQ group and no cross‐reactivity (<0.1%) to 2 antibiotics of the AP group. The ICA allowed detection of CIP and CAP in a panel of dairy products by employing a simple procedure of preliminary sample preparation. The detection limits for the two antibiotics were the same as in PBST. The analytical recoveries of CIP and CAP in dairy products ranged from 83% to 120%. CONCLUSION The analytical characteristics of the test system allow its use for the detection of antibiotics in milk and dairy products during all steps of production. © 2019 Society of Chemical Industry

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Fluorescence studies of the interaction between chloramphenicol and nitrogen‐doped graphene quantum dots and determination of chloramphenicol in chicken feed

Tsai

Hsieh

et al. 2019

J Chinese Chemical Soc

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Chloramphenicol (CAP) is a veterinary antibiotic that has been banned due to its severe side effects in humans. Through the application of manure, veterinary antibiotics can enter the soil, where they can be taken up by crops and vegetables and pose a potential health hazard to humans. Thus, it is highly desirable to develop a rapid and sensitive tool for on-site detection of CAP to ensure food safety and to control the abuse of antibiotics. To this end, nitrogen-doped graphene quantum dots (N-GQDs) were successfully prepared via microwave-assisted synthesis using citric acid and urea as carbon and nitrogen sources, respectively. Analytical results suggested that the interaction between N-GQDs and CAP could occurs via π-π stacking, which quenched N-GQD fluorescence. CAP spiked into chicken feed could be rapidly extracted with ethanol and quantified based on N-GQD fluorescence quenching without further separation. This method showed good recovery (97-102.6%), a low detection limit (1.8 ppm), and was not affected by interference from florfenicol, and thiamphenicol, legal substitute antibiotics. This method has excellent potential for determination of CAP in livestock feed and soil. K E Y W O R D Schloramphenicol, fluorescence quenching, graphene quantum dots, nitrogen doping

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Advances on the chromatographic determination of amphenicols in food

Cited by 46 publications

References 97 publications

Florfenicol-induced Mitochondrial Dysfunction Suppresses Cell Proliferation and Autophagy in Fibroblasts

Florfenicol-induced Mitochondrial Dysfunction Suppresses Cell Proliferation and Autophagy in Fibroblasts

Development of a multicomponent immunochromatographic test system for the detection of fluoroquinolone and amphenicol antibiotics in dairy products

Fluorescence studies of the interaction between chloramphenicol and nitrogen‐doped graphene quantum dots and determination of chloramphenicol in chicken feed

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