Fluorescence studies of the interaction between chloramphenicol and nitrogen‐doped graphene quantum dots and determination of chloramphenicol in chicken feed

Abstract

Chloramphenicol (CAP) is a veterinary antibiotic that has been banned due to its severe side effects in humans. Through the application of manure, veterinary antibiotics can enter the soil, where they can be taken up by crops and vegetables and pose a potential health hazard to humans. Thus, it is highly desirable to develop a rapid and sensitive tool for on-site detection of CAP to ensure food safety and to control the abuse of antibiotics. To this end, nitrogen-doped graphene quantum dots (N-GQDs) were successfully prepared via microwave-assisted synthesis using citric acid and urea as carbon and nitrogen sources, respectively. Analytical results suggested that the interaction between N-GQDs and CAP could occurs via π-π stacking, which quenched N-GQD fluorescence. CAP spiked into chicken feed could be rapidly extracted with ethanol and quantified based on N-GQD fluorescence quenching without further separation. This method showed good recovery (97-102.6%), a low detection limit (1.8 ppm), and was not affected by interference from florfenicol, and thiamphenicol, legal substitute antibiotics. This method has excellent potential for determination of CAP in livestock feed and soil. K E Y W O R D Schloramphenicol, fluorescence quenching, graphene quantum dots, nitrogen doping

Facile Synthesis of N, S-Doped Carbon Quantum Dots from Food Waste as Fluorescent Probe for Sensitive Detection of Thiamphenicol and Its Analogues in Real Food Samples along with an Application in Bioimaging

“…On the contrary, the increase in temperature may cause the stability of the complex to decrease, thereby reducing the degree of static quenching. Comparing the Stern–Volmer quenching constant (KSV) according to the Stern–Volmer equation, the slope obtained for the data recorded at high temperature is lower than that at low temperature ( Table S4 ), which suggests that the mechanism of TAP-induced quenching of N, S-CDs excludes the possibility of dynamic collisions but was attributed to the formation of complexes [ 11 ]. The zeta potential of N, S-CDs was measured to be nearly −17.4 mV, which was attributed to the large number of nitrogen hydrogen bond and carbonyl groups present on the surface of N, S-CDs.…”

“…Fluorescence sensing detection method has been widely used due to excellent optical performance and high sensitivity in recent years. Several studies have shown that TAP detection techniques by semiconductor metal-quantum dot can obtain acceptable linear range and detection [ 11 , 12 ]. However, semiconductor quantum dots are primarily composed of II–VI or III–V metal elements, which still have biosecurity threats.…”

Recent progress on graphene quantum dots‐based fluorescence sensors for food safety and quality assessment applications

“…However, many poultry and cattle farmers continue to use CAP illegally. Tsai et al prepared N-GQDs from citric acid and urea by microwave-assisted synthesis and explored the fluorescence property of N-GQDs toward selective detection of CAP (Tsai et al, 2020). The sensing principle involves the π-π stacking interaction between the N-GQDs and CAP, which resulted in the quenching of N-GQD fluorescence.…”

Potential-Resolved Electrochemiluminescence Multiplex Immunoassay for Florfenicol and Chloramphenicol in a Single Sample

“…4 However, CAP has tremendous toxic effects and is readily enriched in animals and humans, which may cause severe and irreversible myelosuppressive reactions resulting in aplastic anemia in humans and animals. 5 For this reason, CAP is explicitly prohibited for animal feeding and treatment in many countries, including Japan, America, and China. 6 Nevertheless, due to economic interests, some unscrupulous merchants still use CAP in animal husbandry and aquaculture, seriously endangering people's health.…”