Advances in vascularization and innervation of constructs for neural tissue engineering

Frisch, Abigail Newman; Debbi, Lior; Shuhmaher, Margarita; Guo, Shengming; Levenberg, Shulamit

doi:10.1016/j.copbio.2021.08.012

Cited by 7 publications

(5 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An upgrade that could be incorporated into the concept of our implant is a prior seeding of the modules with HUVEC, which can give rise to a pre-vascularization inside the conduit [80]. This pre-vascularization of scaffolds can improve the construct maturity and the blood perfusion after its implantation in vivo [81,82]. Long NGC require an adequate blood supply throughout their entire length, and the central zone usually lacks it.…”

Section: In Vivo Sciatic Nerve Regenerationmentioning

confidence: 99%

Novel Tissue-Engineered Multimodular Hyaluronic Acid-Polylactic Acid Conduits for the Regeneration of Sciatic Nerve Defect

et al. 2022

View full text Add to dashboard Cite

The gold standard for the treatment of peripheral nerve injuries, the autograft, presents several drawbacks, and engineered constructs are currently suitable only for short gaps or small diameter nerves. Here, we study a novel tissue-engineered multimodular nerve guidance conduit for the treatment of large nerve damages based in a polylactic acid (PLA) microfibrillar structure inserted inside several co-linear hyaluronic acid (HA) conduits. The highly aligned PLA microfibers provide a topographical cue that guides axonal growth, and the HA conduits play the role of an epineurium and retain the pre-seeded auxiliary cells. The multimodular design increases the flexibility of the device. Its performance for the regeneration of a critical-size (15 mm) rabbit sciatic nerve defect was studied and, after six months, very good nerve regeneration was observed. The multimodular approach contributed to a better vascularization through the micrometrical gaps between HA conduits, and the pre-seeded Schwann cells increased axonal growth. Six months after surgery, a cross-sectional available area occupied by myelinated nerve fibers above 65% at the central and distal portions was obtained when the multimodular device with pre-seeded Schwann cells was employed. The results validate the multi-module approach for the regeneration of large nerve defects and open new possibilities for surgical solutions in this field.

show abstract

Section: In Vivo Sciatic Nerve Regenerationmentioning

confidence: 99%

Novel Tissue-Engineered Multimodular Hyaluronic Acid-Polylactic Acid Conduits for the Regeneration of Sciatic Nerve Defect

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The angiogenic peptides functionalized biomaterials have been proved by promoting adhesion, cell migration, proliferation, and organization [ 58 ]. The complexity and controllability must be fully considered to construct vascularized grafts that are sufficiently intricate to mimic the natural blood vessel structures in vivo [ 8 ].…”

Section: Discussionmentioning

confidence: 99%

“…Currently, there is no off-the-shelf biomaterials can efficiently induce angiogenesis, and proangiogenic drugs typically suffer from their abnormal angiogenesis and potential cancer risk [ 6 ]. The tissue engineered prevascularization techniques (as coculture, cell sheet technology, spheroids, three-dimensional (3D) bioprinting, and microfluidic technology [ 3 , [7] , [8] , [9] ]) mainly base on: ①vascular endothelial cells (VECs) [ 10 , 11 ] (or VECs differentiated from stem cell [ 12 , 13 ]) or various types of cells as VECs, fibroblasts, and SCs [ 11 ], and then transplanting vascularized engineered grafts enter the body and coincide with the angiogenesis of the host; ② growth factors or cytokines that promote angiogenesis include VEGF, bFGF, HGF, etc. [ [14] , [15] , [16] ].…”

Section: Introductionmentioning

confidence: 99%

Neural tissue-engineered prevascularization in vivo enhances peripheral neuroregeneration via rapid vascular inosculation

Wang

Zhang

et al. 2023

Materials Today Bio

View full text Add to dashboard Cite

“…Current neural science has advanced a lot but still has some unmet needs. Common surgeries cannot mimic microenvironments, micro alignments, or some functionalities, as well as the high cost for customized flexible treatments while 3D printing or hybrid bioprinting technologies hold great promise [27,158]. The 3D printing offers an ideal option to replicate the complex nerve-like structures for the re-establishment of functional neural connections by not only implantation in vivo but also model fabrication in vitro.…”

Section: Typical Applications Of 3d-printed Bioengineered Constructs ...mentioning

confidence: 99%

3D printing of functional bioengineered constructs for neural regeneration: a review

Zhu

Wei

et al. 2023

Int. J. Extrem. Manuf.

View full text Add to dashboard Cite

3D printing technology has opened a new paradigm to controllably and reproducibly fabricate bioengineered neural constructs for potential applications in repairing injured nervous tissues or producing in vitro nervous tissue models. However, the complexity of nervous tissues poses great challenges to three-dimensional (3D)-printed bioengineered analogues, which should possess diverse architectural/chemical/electrical functionalities to resemble the native growth microenvironments for functional neural regeneration. In this work, we provide a state-of-the-art review of the latest development of 3D printing for bioengineered neural constructs. Various 3D printing techniques for neural tissue-engineered scaffolds or living cell-laden constructs are summarized and compared in terms of their unique advantages. We highlight the advanced strategies by integrating topographical, biochemical and electroactive cues inside 3D-printed neural constructs to replicate in vivo-like microenvironment for functional neural regeneration. The typical applications of 3D-printed bioengineered constructs for in vivo repair of injured nervous tissues, bio-electronics interfacing with native nervous system, neural-on-chips as well as brain-like tissue models are demonstrated. The challenges and future outlook associated with 3D printing for functional neural constructs in various categories are discussed.

show abstract

Advances in vascularization and innervation of constructs for neural tissue engineering

Cited by 7 publications

References 45 publications

Novel Tissue-Engineered Multimodular Hyaluronic Acid-Polylactic Acid Conduits for the Regeneration of Sciatic Nerve Defect

Novel Tissue-Engineered Multimodular Hyaluronic Acid-Polylactic Acid Conduits for the Regeneration of Sciatic Nerve Defect

Neural tissue-engineered prevascularization in vivo enhances peripheral neuroregeneration via rapid vascular inosculation

3D printing of functional bioengineered constructs for neural regeneration: a review

Contact Info

Product

Resources

About