Advances in the Pathogenesis of Adhesion Development: The Role of Oxidative Stress

Awonuga, Awoniyi O.; Belotte, J; Abuanzeh, S.; Fletcher, N.M.; Diamond, Michael P.; Saed, Ghassan M.

doi:10.1177/1933719114522550

Cited by 65 publications

(70 citation statements)

References 137 publications

(191 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…After postoperative days 5 and 7, these adhesive strips progressed to persistent fibrous adhesions 2,13 . It was also reported that hypoxia directly promotes the production of free oxygen radicals in tissues 2,14 . With the increase in the production of free oxygen radicals, the production of fibroblasts, tissue growth factor, vascular endothelial growth factor, and cytokines such as cyclooxygenase-2 that cause adhesions also increased 14,15 .…”

Section: ■ Discussionmentioning

confidence: 99%

“…It was also reported that hypoxia directly promotes the production of free oxygen radicals in tissues 2,14 . With the increase in the production of free oxygen radicals, the production of fibroblasts, tissue growth factor, vascular endothelial growth factor, and cytokines such as cyclooxygenase-2 that cause adhesions also increased 14,15 . As a result, histopathologically, inflammation and fibrosis occur [16][17][18] .…”

Section: ■ Discussionmentioning

confidence: 99%

“…As a result, histopathologically, inflammation and fibrosis occur [16][17][18] . There are studies indicating that fibroblasts reduce antioxidant levels similar to free oxygen radicals [14][15][16][17][18] . If the production of free oxygen radicals was reduced using antioxidants, adhesive fibroblasts transformed into normal fibroblasts and collagen production decreased 15 .…”

Section: ■ Discussionmentioning

confidence: 99%

See 2 more Smart Citations

The effect of pycnogenol on lymphatic nodes and adhesion during in a peritoneal adhesion model in rats

Göret¹,

Göret

Kiraz

et al. 2018

Acta Cir. Bras.

View full text Add to dashboard Cite

Purpose: To investigate the effects of pycnogenol on peritoneal adhesions and additionally to investigate the immunohistochemical effects of free oxygen radicals and reactive lymph nodes detected in the adhesive tissue that was sampled surrounding the cecum on intraabdominal adhesions. Methods: Twenty-seven Wistar Albino rats were divided into three groups. In group 1 (sham), laparotomy was performed and stitched up. In group 2 (control), after laparotomy was performed, punctate hemorrhage was induced by cecal abrasion in the cecum and each rat was intraperitoneally administered 2 cc of saline. In group 3 (experimental), after laparotomy was performed, punctate hemorrhage was induced by cecal abrasion in the cecum and each rat was intraperitoneally administered a sterile Pycnogenol derivative. The rats in all groups were re-laparotomized on postoperative day 7; samples were obtained from the peritoneal tissue surrounding the cecum, and the rats were sacrificed. Results: In group 3, there was a statistically significant difference in terms of inflammation, lymph node size, and free oxygen radicals; these parameters tended to increase. In terms of fibrosis evaluated using H&E and MT, there was no significant difference between groups 2 and 3. Conclusions: No positive outcomes indicating that pycnogenol reduces intra-abdominal adhesions were obtained. However, it caused severe inflammation in the tissue. Moreover, a significant increase in lymph node size was detected secondary to inflammation. Additionally, in immunohistochemical analyses conducted to detect oxidative stress, pycnogenol increased the production of free oxygen radicals in the tissue.

show abstract

Section: ■ Discussionmentioning

confidence: 99%

Section: ■ Discussionmentioning

confidence: 99%

Section: ■ Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The effect of pycnogenol on lymphatic nodes and adhesion during in a peritoneal adhesion model in rats

Göret¹,

Göret

Kiraz

et al. 2018

Acta Cir. Bras.

View full text Add to dashboard Cite

show abstract

“…Reactive oxygen radicals (ROS) formed during hypoxia activate fibroblasts, causing the production of cytokines such as vascular endothelial growth factor (VEGF), tissue growth factor (TGF), and cyclooxygenase-2, which all increase adhesion formation [15,16] .…”

Section: Discussionmentioning

confidence: 99%

Uterotonik Bir Ajandan Fazlası: Oksitosin Postoperatif Adezyonları Önlüyor

Işık¹,

Şahbaz²,

Aynıoğlu³

et al. 2016

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

Prevention of postoperative adhesions (PPA) has become an important issue. The aim is to investigate the effect of Oxytocin (OT) on PPAs. A total of thirty female Wistar-albino rats were randomly divided into three groups (10 rats/group). The cecal peritone of Group I rats (controls) were scraped, to trigger adhesion formation, and no treatment were given. After cecal scrubbing, 1 mL saline solution was applied to each rat in Group II (i.p. saline treated group) and 80 IU/kg of OT (Pituisan®, Ege Vet, Turkey) to Group III (i.p. OT treated group) intraperitoneally. All animals were sacrificed 10 days after surgery and adhesions graded in terms of severity and histopathologic characteristics. The median scores for the extent, severity, and degree of adhesions in Group I and Group II were statistically significant and considerably higher than those scores for Group III (P<0.001). The inflammation, neovascularization, and fibrosis scores for Group III were statistically significant and considerably lower than those scores for Groups I and II (P<0.001, P<0.001 and P=0.002 respectively). OT, significantly prevented adhesion formation improving wound healing possibly by suppressing adhesion formation with anti-inflammatory and antioxidant properties. OT may be useful in the prevention of PPA in humans.

show abstract

“…32,33 For the detoxification of these molecules, cells synthesize various types of ROS-scavenging (ie, antioxidant) enzymes. In addition, several studies have pointed to a role for ROS in postoperative adhesion formation, 34 since the administration of ROS scavengers, such as catalase, superoxide dismutase, and trimetazidine, decreased adhesion formation in several animal models. 32,33 As shown in Figure 4A, both pure PLGA and E-PLGA nanofiber membranes were shown to generate few, if any, ROS, but their ROS-scavenging ability was found to be very different from each other.…”

Section: In Vitro Antioxidant Activity and Hemocompatibilitymentioning

confidence: 99%

PLGA nanofiber membranes loaded with epigallocatechin-3-O-gallate are beneficial to prevention of postsurgical adhesions

Shin¹,

Yang²,

Lee³

et al. 2014

IJN

View full text Add to dashboard Cite

This study concentrates on the development of biodegradable nanofiber membranes with controlled drug release to ensure reduced tissue adhesion and accelerated healing. Nanofibers of poly(lactic- co -glycolic acid) (PLGA) loaded with epigallocatechin-3- O -gallate (EGCG), the most bioactive polyphenolic compound in green tea, were electrospun. The physicochemical and biomechanical properties of EGCG-releasing PLGA (E-PLGA) nanofiber membranes were characterized by atomic force microscopy, EGCG release and degradation profiles, and tensile testing. In vitro antioxidant activity and hemocompatibility were evaluated by measuring scavenged reactive oxygen species levels and activated partial thromboplastin time, respectively. In vivo antiadhesion efficacy was examined on the rat peritonea with a surgical incision. The average fiber diameter of E-PLGA membranes was approximately 300–500 nm, which was almost similar to that of pure PLGA equivalents. E-PLGA membranes showed sustained EGCG release mediated by controlled diffusion and PLGA degradation over 28 days. EGCG did not adversely affect the tensile strength of PLGA membranes, whereas it significantly decreased the elastic modulus and increased the strain at break. E-PLGA membranes were significantly effective in both scavenging reactive oxygen species and extending activated partial thromboplastin time. Macroscopic observation after 1 week of surgical treatment revealed that the antiadhesion efficacy of E-PLGA nanofiber membranes was significantly superior to those of untreated controls and pure PLGA equivalents, which was comparable to that of a commercial tissue-adhesion barrier. In conclusion, the E-PLGA hybrid nanofiber can be exploited to craft strategies for the prevention of postsurgical adhesions.

show abstract

Advances in the Pathogenesis of Adhesion Development: The Role of Oxidative Stress

Cited by 65 publications

References 137 publications

The effect of pycnogenol on lymphatic nodes and adhesion during in a peritoneal adhesion model in rats

The effect of pycnogenol on lymphatic nodes and adhesion during in a peritoneal adhesion model in rats

Uterotonik Bir Ajandan Fazlası: Oksitosin Postoperatif Adezyonları Önlüyor

PLGA nanofiber membranes loaded with epigallocatechin-3-O-gallate are beneficial to prevention of postsurgical adhesions

Contact Info

Product

Resources

About