Advances in sperm cryopreservation in farm animals: Cattle, horse, pig and sheep

Yánez-Ortiz, Iván; Catalán, Jaime; Rodríguez‐Gil, Joan E.; Miró, Jordi; Yeste, Marc

doi:10.1016/j.anireprosci.2021.106904

Cited by 73 publications

(57 citation statements)

References 204 publications

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“…However, a large number of studies implicated that differences exist regarding the optimum level of glycerol during cryopreservation in different species. For most species, the recommended level of glycerol is 3-5% [16]. In the case of buffalo semen cryopreservation, 6 to 7% of glycerol concentration is recommended [17].…”

Section: Discussionmentioning

confidence: 99%

“…Another notable constituent that contributed to the increased efficacy of TFE is egg yolk. Most diluters are composed of 20% egg yolk [16] and a previous study reported that reducing its proportion to 10% considerably vitiated the semen quality of animals [23]. The presence of low-density lipoproteins in the egg yolk mainly renders protection to the sperms by forming a layer around the plasma membrane.…”

Section: Discussionmentioning

confidence: 99%

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Comparison between Manual and Automated Cryopreservation Techniques and Effects of Different Semen Extenders on Post-Thaw Semen Quality of Buffalo

Kabir¹,

Hossain²,

Deb³

et al. 2022

Preprint

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Cryopreservation has been used extensively for cattle in Bangladesh, albeit no study was conducted on the cryopreservation techniques of buffalo. This study compares two freezing methods and the effects of diluters on the semen quality of buffalo. In the first freezing protocol, semen was frozen in two-step: from 37 °C to 5 °C for 30 minutes in a BLRI-developed equilibration chamber and from 5 oC to -120 oC in a Styrofoam box using liquid nitrogen vapor from different distances (0.5, 1.5, 1.6, 2 and 3 inches). At the same time semen was frozen in a programmable freezer in three steps. The semen samples were then evaluated for motility and morphological quality by CASA. In another experiment, the efficacy of one locally developed diluter-Tris-fructose-egg yolk-based (TFE) and three commercial diluters (Andromed, Triladyl and Steridyl) were evaluated. The highest number of motile sperms (62.67±1.12; P< 0.01) and progressive motility (38.97±1.10; P < 0.001) was observed at 1.6 inches above liquid nitrogen. There was no significant difference in overall motility, progressive and slow motility between semen cryopreserved in the cheap nitrogen vapor technique (57.49±5.67, 38.70± 4.04 and 3.83± 0.63, respectively) and expensive automated technique (65.94±4.65, 45.54 ± 3.64 and 2.43± 0.36, respectively). The highest recovery rate and conception rate were observed in semen diluted with TFE (82.4% and 80%, respectively). Hence, the cryopreservation technique using nitrogen vapor and TFE diluent is cost-effective and suitable for freezing buffalo semen that would produce superior semen for artificial insemination.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Comparison between Manual and Automated Cryopreservation Techniques and Effects of Different Semen Extenders on Post-Thaw Semen Quality of Buffalo

Kabir¹,

Hossain²,

Deb³

et al. 2022

Preprint

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show abstract

“…Various types of stress, including osmotic and thermal stress, are predominant during thawing and freezing procedures and affect sperm response and efficiency. Seminal plasma (SP) proteins affect sperm’s ability to cryopreserve farm animals, such as cattle, pigs, horses and sheep [ 57 ]. Sperm cryopreservation is affected by these SP proteins, as they vary from individual to individual [ 58 ].…”

Section: Markers Of Freezabilitymentioning

confidence: 99%

Effect of Sperm Cryopreservation in Farm Animals Using Nanotechnology

Akhtar

et al. 2022

Animals

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Sperm cryopreservation is one of the sublime biotechnologies for assisted reproduction. In recent decades, there has been an increasing trend in the use of preserved semen. Post-thaw semen quality and values vary among animals of the same species. Similarly, there are species-specific variations in sperm morphology, i.e., sperm head, kinetic properties, plasma membrane integrity, and freezability. Similarly, the viability of sperm varies in the female reproductive tract, i.e., from a few hours (in cattle) to several days (in chicken). Various steps of sperm cryopreservation, i.e., male health examination, semen collection, dilution, semen centrifugation, pre- and post-thaw semen quality evaluation, lack standardized methodology, that result in differences in opinions. Assisted reproductive technologies (ART), including sperm preservation, are not applied to the same extent in commercial poultry species as in mammalian species for management and economic reasons. Sperm preservation requires a reduction in physiological metabolism by extending the viable duration of the gametes. Physiologically and morphologically, spermatozoa are unique in structure and function to deliver paternal DNA and activate oocytes after fertilization. Variations in semen and sperm composition account for better handling of semen, which can aid in improved fertility. This review aims to provide an update on sperm cryopreservation in farm animals.

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“…Cryopreservation is a simple, economical, and efficient method for preserving the biological and genetic resources of sperm (Yánez-Ortiz et al, 2021). At present, it has been used in many fields and has become an essential means to treat human infertility, livestock reproduction, and biodiversity conservation of biological resources (Yánez-Ortiz et al, 2021;Aliakbari et al, 2021). However, sperm damage caused by cryopreservation cannot be ignored, such as cold shock, mitochondrial damage, acrosome damage, and others (Chaudhari et al, 2015;Yoon et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Effect of glycerol addition time on the cryopreserved Korean native brindle cattle (Chikso) sperm quality

Kim²,

Jung

et al. 2022

Anim. Reprod.

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Although cryopreservation is an efficient method for maintaining the biological and genetic resources of sperm, the sperm damage during the cryopreservation process cannot be ignored. It should be possible to obtain the most effective cryopreservation performance by accurately grasping the effects of various factors on the cryopreservation of sperm. The previous study demonstrated that a suitable standard protocol for cryopreservation of Korean native brindled cattle (Chikso) does not exist, based on the methods for semen cryopreservation of Chikso differ in each research center. The most obvious difference between most of protocols is the addition of glycerol before and after cooling during the Chikso cryopreserved semen process. Therefore we focused on the effects of glycerol addition time on the quality of cryopreserved Chikso sperm. In the present study, 27 individual Chikso samples were collected by transrectal massage and divided into two parts: the "cryopreservation method A" group (adding glycerol before cooling) and the "cryopreservation method B" group (adding glycerol after cooling). Meanwhile, the values of various sperm parameters were derived from each group, including sperm motility, kinematics, capacitation status, cell viability, and intracellular ATP levels, which we used to compare and evaluate sperm function. The results of this study indicated that during the semen cryopreservation process of the Chikso, the addition of glycerol after cooling yielded superior results in a variety of sperm parameters, such as sperm motility, progressive motility, rapid motility, VCL, VSL, VAP, ALH, capacitation status, viability, and intracellular ATP level after freezing and thawing. Our study is suggested that the glycerol addition time during the cryopreservation process for Chikso should be considered. In addition, our results may be provided reference to develop suitable the cryopreservation procedure of the Chikso sperm.

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Advances in sperm cryopreservation in farm animals: Cattle, horse, pig and sheep

Cited by 73 publications

References 204 publications

Comparison between Manual and Automated Cryopreservation Techniques and Effects of Different Semen Extenders on Post-Thaw Semen Quality of Buffalo

Comparison between Manual and Automated Cryopreservation Techniques and Effects of Different Semen Extenders on Post-Thaw Semen Quality of Buffalo

Effect of Sperm Cryopreservation in Farm Animals Using Nanotechnology

Effect of glycerol addition time on the cryopreserved Korean native brindle cattle (Chikso) sperm quality

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