Advances in skeletal tissue engineering with hydrogels

Elisseeff, Jennifer H.; Puleo, Christopher M.; Yang, Fan; Bk, Sharma

doi:10.1111/j.1601-6343.2005.00335.x

Cited by 131 publications

(110 citation statements)

References 93 publications

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“…Due to their high water content, mechanical properties of hydrogels often closely match those of biological tissues. Hydrogels have been traditionally used for a range of soft tissue replacement applications such as contact lenses, drug delivery vehicles, separation membranes and adhesives [29][30][31][32]. Table 1 lists representative natural and synthetic polymers used in medical devices.…”

Section: Introductionmentioning

confidence: 99%

Conducting polymer-hydrogels for medical electrode applications

Green

Baek

Poole-Warren

et al. 2010

Science and Technology of Advanced Materials

241

182

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Conducting polymers hold significant promise as electrode coatings; however, they are characterized by inherently poor mechanical properties. Blending or producing layered conducting polymers with other polymer forms, such as hydrogels, has been proposed as an approach to improving these properties. There are many challenges to producing hybrid polymers incorporating conducting polymers and hydrogels, including the fabrication of structures based on two such dissimilar materials and evaluation of the properties of the resulting structures. Although both fabrication and evaluation of structure-property relationships remain challenges, materials comprised of conducting polymers and hydrogels are promising for the next generation of bioactive electrode coatings.

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Section: Introductionmentioning

confidence: 99%

Conducting polymer-hydrogels for medical electrode applications

Green

Baek

Poole-Warren

et al. 2010

Science and Technology of Advanced Materials

241

182

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“…Hydrogels are a class of biomaterial that are commonly and widely used in cartilage tissue engineering and include alginate, agarose, poly(ethylene glycol) (PEG), poly(vinyl alcohol) (PVA), pluronics, chitosan, collagen and fibrin as examples (30,31). Hydrogels provide significant advantages over traditional porous-type sponges including high water content, efficient transport of nutrients and waste removal, and possess the ability to effectively and homogeneously encapsulate cells as they are generally mixed with the gel prior to gelation.…”

Section: Introductionmentioning

confidence: 99%

Engineering of Large Cartilaginous Tissues Through the Use of Microchanneled Hydrogels and Rotational Culture

Buckley

Thorpe

Kelly

2009

Tissue Engineering Part A

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The development of functional engineered cartilaginous tissues of sufficient size that can be used clinically to treat large defects remains a major and significant challenge. This study investigated if the introduction of microchannels into chondrocyte-seeded agarose hydrogels would result in the formation of a superior and more homogenous cartilaginous tissue due to enhanced nutrient transport. Microchannel construct cylinders were fabricated via a moulding process utilising a pillared structure to create the required architecture. Constructs were subjected to either constant rotation in a rotational bioreactor system or free swelling conditions. After 28 days of free swelling culture the presence of microchannels did not enhance GAG accumulation within the core of the construct compared to solid constructs (0.317 ± 0.002 % w/w vs. 0.401 ± 0.020 % w/w). However under dynamically rotating conditions, GAG accumulation in the cores (1.165 ± 0.132 % w/w) of microchannel constructs were similar to that in the periphery (1.23 ± 0.074 % w/w) of solid constructs, although still significantly lower than their corresponding periphery (1.64 ± 0.133 % w/w) after 28 days. These results confirm that cellular nutrient consumption is primarily responsible for creating the spatial gradients in molecules regulating the biosynthetic activity of chondrocytes through the volume of hydrogels, and that changing the scaffold architecture alone may have little effect while the inherent diffusivity of the material remains high. Rather a combination of forced convection and modified scaffold architecture is necessary to engineer large cartilaginous tissues in vitro.

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“…It has been known for more than two decades that hydrogels support the spherical shape and normal phenotype of chondrocytes [35]. Based on these early findings, many studies have used hydrogels, such as agarose, alginate, chitosan and photopolymerizing systems, to engineer cartilage tissue [4,36,37]. In a study of human chondrocytes in six different scaffolds, the highest matrix production was recorded in collagen gel [38].…”

Section: Biomaterials Scaffoldsmentioning

confidence: 99%

“…Osteochondral defects resulting from traumatic injuries, osteochondritis dissecans and chondromalacia further contribute to the pool of patients that need surgical treatment. Standard procedures, such as total joint replacement using artificial materials, are generally successful in terms of pain relief and improved function but do not restore the articular cartilage and subchondral bone completely and degenerate over time [4]. Mosaicplasty involves taking osteochondral plugs from a non-load-bearing area of the patient's own joint (autografts) and transplanting these plugs into the disease site.…”

Section: Clinical Problem and Current Approachesmentioning

confidence: 99%

Engineering custom-designed osteochondral tissue grafts

Grayson

Chao

Marolt

et al. 2008

Trends in Biotechnology

123

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Tissue engineering is expected to help us outlive the failure of our organs by enabling the creation of tissue substitutes capable of fully restoring the original tissue function. Degenerative joint disease, which affects one-fifth of the US population and is the country's leading cause of disability, drives current research of actively growing, functional tissue grafts for joint repair. Toward this goal, living cells are used in conjunction with bio-material scaffolds (serving as instructive templates for tissue development) and bioreactors (providing environmental control and molecular and physical regulatory signals). In this review, we discuss the requirements for engineering customized, anatomically-shaped, stratified grafts for joint repair and the challenges of designing these grafts to provide immediate functionality (load bearing, structural support) and long-term regeneration (maturation, integration, remodeling).

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Advances in skeletal tissue engineering with hydrogels

Abstract: Tissue engineering provides a venue to investigate tissue development of mutant or diseased cells and potential therapeutics.

Cited by 131 publications

References 93 publications

Conducting polymer-hydrogels for medical electrode applications

Conducting polymer-hydrogels for medical electrode applications

Engineering of Large Cartilaginous Tissues Through the Use of Microchanneled Hydrogels and Rotational Culture

Engineering custom-designed osteochondral tissue grafts

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