Advances in Protein Production Technologies

Lua, Linda H.L.; Chuan, Yap P.

doi:10.1002/9783527653096.ch2

Biopharmaceutical Production Technology 2012

DOI: 10.1002/9783527653096.ch2

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Advances in Protein Production Technologies

Linda H.L. Lua

Yap P. Chuan

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Cited by 2 publications

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“…The biopharmaceutical industry is a multibillion-dollar industry which produces proteins and other drugs from microorganism fermentation 1 2 3 . Major side products from the fermentation processes are the endotoxins produced by Gram-negative bacteria, present in the fermentation broth and which can occur at any point within the process 4 .…”

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Phospholipid imprinted polymers as selective endotoxin scavengers

Šulc

Székely

Shinde

et al. 2017

Sci Rep

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Herein we explore phospholipid imprinting as a means to design receptors for complex glycolipids comprising the toxic lipopolysaccharide endotoxin. A series of polymerizable bis-imidazolium and urea hosts were evaluated as cationic and neutral hosts for phosphates and phosphonates, the latter used as mimics of the phospholipid head groups. The bis-imidazolium hosts interacted with the guests in a cooperative manner leading to the presence of tight and well defined 1:2 ternary complexes. Optimized monomer combinations were subsequently used for imprinting of phosphatidic acid as an endotoxin dummy template. Presence of the aforementioned ternary complexes during polymerization resulted in imprinting of lipid dimers – the latter believed to crudely mimic the endotoxin Lipid A motif. The polymers were characterized with respect to template rebinding, binding affinity, capacity and common structural properties, leading to the identification of polymers which were thereafter subjected to an industrially validated endotoxin removal test. Two of the polymers were capable of removing endotoxin down to levels well below the accepted threshold (0.005 EU/mg API) in pharmaceutical production.

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Phospholipid imprinted polymers as selective endotoxin scavengers

Šulc

Székely

Shinde

et al. 2017

Sci Rep

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Development of picornavirus-like particle vaccines

Somasundaram¹,

Lua

2015

Pharmaceutical Bioprocessing

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Advances in Protein Production Technologies

Cited by 2 publications

References 203 publications

Phospholipid imprinted polymers as selective endotoxin scavengers

Phospholipid imprinted polymers as selective endotoxin scavengers

Development of picornavirus-like particle vaccines

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