Development of picornavirus-like particle vaccines

Somasundaram, Balaji; Lua, Linda H.L.

doi:10.4155/pbp.14.39

Cited by 4 publications

(7 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Compared to common prokaryotic expression systems, eukaryotic expression systems offer more advantages for VLP production [ 9 ]. Baculovirus-based insect cell expression systems are commonly used for developing VLP vaccines [ 10 , 11 , 12 ]. In insect cells, the process of protein production and transformation is often similar to that in humans, and they can produce a variety of VLPs.…”

Section: Introductionmentioning

confidence: 99%

“…The mechanism of VP0 cleavage is poorly understood. Therefore, the protein expression of P1 and 3CD must be regulated for HFMD-VLPs, and various expression strategies have been attempted accordingly [ 12 , 14 , 15 , 16 , 17 , 18 ]. For the first time, the following VLPs generated by co-expressing two types of viruses were reported: P1-expressing viruses generated using the polyhedrin (polh ) promoter and 3CD-expressing viruses generated using the p10 promoter [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the protein expression of P1 and 3CD must be regulated for HFMD-VLPs, and various expression strategies have been attempted accordingly [ 12 , 14 , 15 , 16 , 17 , 18 ]. For the first time, the following VLPs generated by co-expressing two types of viruses were reported: P1-expressing viruses generated using the polyhedrin (polh ) promoter and 3CD-expressing viruses generated using the p10 promoter [ 12 ]. Later, the system was changed to a dual-vector system that simultaneously expresses P1 and 3CD, which can be produced by one virus [ 12 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

“…For the first time, the following VLPs generated by co-expressing two types of viruses were reported: P1-expressing viruses generated using the polyhedrin (polh ) promoter and 3CD-expressing viruses generated using the p10 promoter [ 12 ]. Later, the system was changed to a dual-vector system that simultaneously expresses P1 and 3CD, which can be produced by one virus [ 12 , 14 , 15 ]. Since then, research has been actively conducted to increase the yield of VLP.…”

Section: Introductionmentioning

confidence: 99%

“…Since then, research has been actively conducted to increase the yield of VLP. In particular, as 3CD has been reported to exhibit cytotoxicity, research is mainly conducted to control the expression level of 3CD [ 12 , 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Efficient Production of Enterovirus 71 (EV71) Virus-like Particles by Controlling Promoter Strength in Insect Cells

Kim,

Moon,

Choi

et al. 2024

Viruses

View full text Add to dashboard Cite

This study was conducted to efficiently produce virus-like particles (VLPs) of enterovirus 71 (EV71), a causative virus of hand, foot, and mouth disease (HFMD). The expression level of the P1 precursor, a structural protein of EV71, was modified to increase VLP production, and the optimal expression level and duration of the 3CD protein for P1 cleavage were determined. The expression level and duration of 3CD were controlled by the p10 promoter, which was weakened by repeated burst sequence (BS) applications, as well as the OpIE2 promoter, which was weakened by the insertion of random untranslated region sequences of various lengths. The cleavage and production efficiency of the P1 precursor were compared based on the expression time and level of 3CD, revealing that the p10-BS5 promoter with four repeated BSs was the most effective. When P1 and 3CD were expressed using the hyperexpression vector and the p10-BS5 promoter, high levels of structural protein production and normal HFMD-VLP formation were observed, respectively. This study suggests that the production efficiency of HFMD-VLPs can be significantly enhanced by increasing the expression of the P1 precursor and controlling the amount and duration of 3CD expression.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Efficient Production of Enterovirus 71 (EV71) Virus-like Particles by Controlling Promoter Strength in Insect Cells

Kim,

Moon,

Choi

et al. 2024

Viruses

View full text Add to dashboard Cite

show abstract

Development of sandwich ELISAs that can distinguish different types of coxsackievirus A16 viral particles

Yang

Jia

et al. 2016

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Coxsackievirus A16 (CA16) is one of the major causative agents of hand, foot, and mouth disease (HFMD). No CA16 vaccine candidates have progressed to clinical trials so far. Immunogenicity studies indicated that different CA16 particles have much influence on the efficacy of a candidate vaccine. However, there are still no relevant reports on the methods of detecting different CA16 particles. In this study, we screened several monoclonal antibodies (mAbs) specific for different CA16 particles, and several sandwich enzyme-linked immunoassays (ELISAs) were developed to measure the different types of CA16 viral particles. The mAbs that could only bind denatured or empty capsids could not neutralize CA16. In contrast, the mAbs that could bind mature full particles or all types of particles showed obvious neutralizing activity. The thermal stability of different CA16 particles was evaluated using these sandwich ELISAs. The mature full particles were found to be more thermolabile than the other types of particles and could be stabilized by high concentrations of cations. These methods can be used to assist in the potency control of CA16 vaccines and will promote the development of a CA16 vaccine.

show abstract

A new EV71 VP3 epitope in norovirus P particle vector displays neutralizing activity and protection in vivo in mice

Jiang

Fan

Sun

et al. 2015

Vaccine

View full text Add to dashboard Cite

Development of picornavirus-like particle vaccines

Cited by 4 publications

References 74 publications

Efficient Production of Enterovirus 71 (EV71) Virus-like Particles by Controlling Promoter Strength in Insect Cells

Efficient Production of Enterovirus 71 (EV71) Virus-like Particles by Controlling Promoter Strength in Insect Cells

Development of sandwich ELISAs that can distinguish different types of coxsackievirus A16 viral particles

A new EV71 VP3 epitope in norovirus P particle vector displays neutralizing activity and protection in vivo in mice

Contact Info

Product

Resources

About