Advances in periodontal disease markers

Özmeriç, Nurdan

doi:10.1016/j.cccn.2004.01.022

Cited by 155 publications

(149 citation statements)

References 132 publications

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“…Initial protein analysis of GCF has been elucidated using techniques such as crossed immunoelectrophoresis, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), twodimensional gel electrophoresis coupled with mass spectrometry (MS) and ELISA (Estreicher et al, 1996;Golub and Kleinberg, 1976;Pisano et al, 2005). Using these approaches, more than 60 individual components of GCF have been assessed as candidate markers of disease progression (Lamster and Ahlo, 2007;Ozmeric, 2004;Uitto, 2003). Specifically, plasma proteins, bacterial and host enzymes, various inflammatory mediators have been among the myriad components studied.…”

Section: Introductionmentioning

confidence: 99%

Application of Label-Free Absolute Quantitative Proteomics in Human Gingival Crevicular Fluid by LC/MS^E (Gingival Exudatome)

et al. 2010

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Periodontal disease is perhaps the most common infectious disease in humans. Gingival crevicular fluid (GCF) is a local inflammatory exudate of the periodontal tissues. Its composition greatly varies between health and periodontal disease. GCF collection is rapid and noninvasive, but previous approaches aiming to analyze its composition have mainly involved single protein biomarkers. The aim of this study was to perform analysis of the GCF exudatome from healthy and periodontally diseased sites by LC/MS E , a label-free mass spectrometry method that enables simultaneous protein identification and absolute quantification in biological fluids. In total, 154 proteins of human, bacterial, and viral origin were identified in the 40 GCF samples obtained from the 10 subjects (five healthy and five generalized aggressive periodontitis). The proportion of bacterial, viral, and yeast protein was increased in disease, compared to health. The presence of host defense-related proteins, such as Cystatin-B and defensins, was confirmed to be present only in health. Among the newly identified GCF proteins were L-plastin detected only in disease (15.6 ( 12.1 fmol) and Annexin-1 detected in 5-fold higher levels in health. Nevertheless, pro-inflammatory cytokines or periodontal pathogen proteins were rarely detected. Conclusively, the LC/MS E technology may facilitate characterization of GCF proteome in periodontal health and disease, thus conferring prognostic and diagnostic value. Larger cohort studies are required to characterize the complete GCF proteome in health and disease.

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Section: Introductionmentioning

confidence: 99%

Application of Label-Free Absolute Quantitative Proteomics in Human Gingival Crevicular Fluid by LC/MS^E (Gingival Exudatome)

et al. 2010

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“…It has been suggested that as a result of stimulation by bacterial antigens, PMNs produce and release a large quantity of ROS, culminating in heightened oxidative damage to gingival tissue, periodontal ligament, and alveolar bone (Sculley and Langley-Evans 2002). ROS are active in the depolymerization of extracellular matrix components, lipid peroxidation, oxidation of enzymes such as anti-proteases, induction of pro-inflammatory cytokines, and DNA damage (Canakci et al 2005;Ozmeric 2005).…”

Section: Introductionmentioning

confidence: 99%

Increased salivary level of 8-hydroxydeoxyguanosine is a marker of premature oxidative mitochondrial DNA damage in gingival tissue of patients with periodontitis

Çanakçı

Tatar

et al. 2009

Arch. Immunol. Ther. Exp.

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Introduction: Oxidative stress may contribute to the pathogenesis of periodontitis. However, the detailed molecular mechanism remains unclear. Both 8-hydroxydeoxyguanosine (8-OHdG) and mitochondrial DNA (mtDNA) deletion have been reported as early oxidative DNA damage markers. In this study, 8-OHdG levels in saliva and mtDNA deletions in gingival tissue of patients with chronic periodontitis (CP) were evaluated. Materials and Methods: Gingival tissue and whole saliva samples were collected from 32 patients with CP and 32 healthy control subjects. To determine the clinical condition of each subject, the plaque index, gingival index, clinical attachment level (CAL), and probing depth (PD) were measured. Using the ELISA and polymerase chain reaction methods, the salivary 8-OHdG levels and the 7.4-kbp and 5-kbp mtDNA deletions were examined. Results: The 5-kbp mtDNA deletion was detected in 20 of the 32 periodontitis patients (62.5%), but was not detected in the healthy controls. The mean value of 8-OHdG in the saliva of the periodontitis patients with deleted mtDNA was significantly higher than in the patients with non-deleted mtDNA (p<0.01). Also, significant correlation was found between the occurrence of the 5-kbp mtDNA deletion and salivary 8-OHdG levels (p<0.01). Similar correlations were detected between salivary 8-OHdG levels and age, PD, and CAL (p<0.01, p<0.05). Conclusion: Increased oxidative stress may lead to premature oxidative DNA damage in the gingival tissue of periodontitis patients and the salivary 8-OHdG level may signify premature oxidative mtDNA damage in diseased gingival tissue.

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“…The IgA1 predominates in serum, teeth and implants, and is a compound of sulcular fluid (3,5), while IgA2 is found in higher concentrations in external secretions like saliva (1,5). Both IgAs are found in saliva and sulcular fluid due to the intimate contact between the secretions (2,3,5,6). The high levels of salivary IgA might protect against the development of gingivitis (1,5).…”

Section: Introductionmentioning

confidence: 99%

“…Both IgAs are found in saliva and sulcular fluid due to the intimate contact between the secretions (2,3,5,6). The high levels of salivary IgA might protect against the development of gingivitis (1,5). In the same way, IgA in sulcular fluid seems to have a protective function (4), which may be related to the lack of complement activation (1,3).…”

Section: Introductionmentioning

confidence: 99%

Levels of Immunoglobulin A1 in Peri-Implant Fluid and Saliva from Patients with Mucositis: A Preliminary Study

Moura

Soares²,

Vieira

et al. 2013

Braz. Dent. J.

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There are no studies evaluating the possible use of immunoglobulin A1 (IgA1) as an early marker for peri-implant inflammation. The aim of this study was to evaluate the IgA1 levels in peri-implant sulcular fluid (PISF) and saliva of partially edentulous patients as an indicator of mucositis. Twenty-seven patients were examined to determine the peri-implant status based on probing depth and bleeding on probing. Saliva and PISF around dental implants were collected and the IgA1 levels were evaluated by Elisa assay. IgA1 in saliva and PISF of these patients were compared and their correlations with clinical parameters were evaluated. Differences in IgA1 levels in saliva (821.1 ± 290.6; 779.8 ± 401.5) and PISF (26.6 ± 20.7; 25.1 ± 20.5) of healthy and mucositis groups, respectively were not observed (p>0.05). Correlation between clinical parameters and IgA1 in saliva or PISF was not observed in healthy or mucositis groups (p=0.607; p=0.826, respectively). These results suggest that IgA1 cannot be used as an immunological marker of mucositis.

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Advances in periodontal disease markers

Cited by 155 publications

References 132 publications

Application of Label-Free Absolute Quantitative Proteomics in Human Gingival Crevicular Fluid by LC/MS^E (Gingival Exudatome)

Application of Label-Free Absolute Quantitative Proteomics in Human Gingival Crevicular Fluid by LC/MS^E (Gingival Exudatome)

Increased salivary level of 8-hydroxydeoxyguanosine is a marker of premature oxidative mitochondrial DNA damage in gingival tissue of patients with periodontitis

Levels of Immunoglobulin A1 in Peri-Implant Fluid and Saliva from Patients with Mucositis: A Preliminary Study

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Advances in periodontal disease markers

Cited by 155 publications

References 132 publications

Application of Label-Free Absolute Quantitative Proteomics in Human Gingival Crevicular Fluid by LC/MSE (Gingival Exudatome)

Application of Label-Free Absolute Quantitative Proteomics in Human Gingival Crevicular Fluid by LC/MSE (Gingival Exudatome)

Increased salivary level of 8-hydroxydeoxyguanosine is a marker of premature oxidative mitochondrial DNA damage in gingival tissue of patients with periodontitis

Levels of Immunoglobulin A1 in Peri-Implant Fluid and Saliva from Patients with Mucositis: A Preliminary Study

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Application of Label-Free Absolute Quantitative Proteomics in Human Gingival Crevicular Fluid by LC/MS^E (Gingival Exudatome)

Application of Label-Free Absolute Quantitative Proteomics in Human Gingival Crevicular Fluid by LC/MS^E (Gingival Exudatome)