Advances in on-chip photodetection for applications in miniaturized genetic analysis systems

Namasivayam, Vijay; Lin, Rui‐Biao; Johnson, B. C.; Brahmasandra, Sundaresh N.; Razzacki, Zafar; Burke, David T.; Burns, Mark A.

doi:10.1088/0960-1317/14/1/311

Cited by 72 publications

(45 citation statements)

References 14 publications

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“…Namasivayam et al [37] and Jorgensen et al [38] used a monolithic approach, where the photodiodes were fabricated in the same substrate as the bottom of the microfluidic channels. The first example is an improvement of a previously published highly integrated device for DNA analysis, where an interference filter also was incorporated for suppression of excitation light.…”

Section: Semiconductor Photodetectorsmentioning

confidence: 99%

Recent developments in detection for microfluidic systems

2004

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Microfluidic systems have become more and more important in the field of analytical chemistry. Detection methods on these microsystems are essential for the identification and quantification of chemical species that are being analyzed. This review concentrates on the latest developments of optical detection methods and mass spectrometry in conjunction with microfluidic systems. Electrochemical methods are discussed in another review in the same issue of this journal. Within the optical detection section, topics such as multiplexed detection and the use of waveguides are discussed. Within the discussion of mass spectrometry, the main focus is on electrospray emitters as interfaces between microsystem and spectrometer. Apart from optical detection and mass spectrometry, other techniques such as flame ionization and nuclear magnetic resonance are also mentioned.

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Section: Semiconductor Photodetectorsmentioning

confidence: 99%

Recent developments in detection for microfluidic systems

2004

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“…Chabinyc et al 102 presented an avalanche photodiode coupled to a PDMS microdevice using a fiber optic cable. Namasivayam et al 103 have investigated the use of Si photodiodes for on-chip fluorescence detection and have fabricated these within integrated genetic analysis systems. It is important to note that the choice of substrates plays an important role in the integration of optoelectronic components.…”

Section: Integrated Opticsmentioning

confidence: 99%

Integrated Genetic Analysis Microsystems

Lagally

Soh

2005

Critical Reviews in Solid State and Materials Sciences

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The advent of integrated microsystems for genetic analysis allows the acquisition of information at unprecedented length and time scales. The convergence of molecular biology, chemistry, physics, and materials science is required for their design and construction. The utility of the microsystems originates from increased analysis speed, lower analysis cost, and higher parallelism leading to increased assay throughput. In addition, when fully integrated, this technology will enable portable systems for high-speed in situ analyses, permitting a new standard in disciplines such as clinical chemistry, personalized medicine, forensics, biowarfare detection, and epidemiology. This article presents an overview of the recent history of integrated genetic analysis microsystems with an emphasis on materials aspects, and provides a perspective on current developments and future prospects.

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“…Integrated microfluidic systems consist of a combination of microfabricated devices [17][18][19], such as micro-scale separators [20,21], chemical reactors [22,23], microvalves and pumps [24,25], and various detection schemes [26][27][28]. An integration challenge is interfacing PCR amplifiers with mCE separators due to the need to transfer the sample into a mCE channel via electrokinetics from a hydrodynamic flow required for the PCR in most cases.…”

Section: Introductionmentioning

confidence: 99%

Integrated continuous flow polymerase chain reaction and micro‐capillary electrophoresis system with bioaffinity preconcentration

et al. 2011

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An integrated and modular DNA analysis system is reported that consists of two modules: (i) A continuous flow polymerase chain reaction (CFPCR) module fabricated in a high T(g) (150°C) polycarbonate substrate in which selected gene fragments were amplified using biotin and fluorescently labeled primers accomplished by continuously shuttling small packets of PCR reagents and template through isothermal zones as opposed to heating and cooling large thermal masses typically performed in batch-type thermal reactors. (ii) μCE (micro-capillary electrophoresis) module fabricated in poly(methylmethacrylate) (PMMA), which utilized a bioaffinity selection and purification bed (2.9 μL) to preconcentrate and purify the PCR products generated from the CFPCR module prior to electrophoretic sorting. Biotin-labeled CFPCR products were hydrostatically pumped through the streptavidin-modified bed, where they were extracted onto the surface of micropillars. The affinity bed was also fabricated in PMMA and was populated with an array of microposts (50 μm width; 100 μm height) yielding a total surface area of ∼117 mm(2). This solid-phase extraction (SPE) process demonstrated high selectivity for biotinylated amplicons and utilized the strong streptavidin/biotin interaction (K(d) = 10(-15) M) to generate high recoveries. The SPE selected CFPCR products were thermally denatured and single-stranded DNA released for injection into a 7-cm-long μCE channel for size-based separations and fluorescence detection. The utility of the system was demonstrated using Alu DNA typing for gender and ethnicity determinations as a model. Compared with the traditional cross-T injection procedure typically used for μCE, the affinity pre-concentration and injection procedure generated signal enhancements of 17- to 40-fold, critical for CFPCR thermal cyclers due to Taylor dispersion associated with their operation.

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Advances in on-chip photodetection for applications in miniaturized genetic analysis systems

Cited by 72 publications

References 14 publications

Recent developments in detection for microfluidic systems

Recent developments in detection for microfluidic systems

Integrated Genetic Analysis Microsystems

Integrated continuous flow polymerase chain reaction and micro‐capillary electrophoresis system with bioaffinity preconcentration

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