Advances in metabolic engineering of cyanobacteria for production of biofuels

Mund, Nitesh Kumar; Liu, Yisong; Chen, Shaolin

doi:10.1016/j.fuel.2022.124117

Cited by 22 publications

(9 citation statements)

References 98 publications

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“…According to the functional annotation with KAAS and the metabolic pathways reconstructed, the genome of the cyanobacterium possesses the totality of genes coding the enzymes involved in de novo fatty acid biosynthesis ( Supplementary Figure S8 ). The type of the fatty acid biosynthesis pathway identified is II FAS, which typically operates in prokaryotic microorganisms such as cyanobacteria ( Mund et al, 2022 ). Recently, it has been demonstrated that the enzymes of the fatty acid biosynthesis pathway form a protein community called metabolon ( Skalidis et al, 2022 ).…”

Section: Resultsmentioning

confidence: 99%

Genomic analysis and biochemical profiling of an unaxenic strain of Synechococcus sp. isolated from the Peruvian Amazon Basin region

et al. 2022

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Cyanobacteria are diverse photosynthetic microorganisms able to produce a myriad of bioactive chemicals. To make possible the rational exploitation of these microorganisms, it is fundamental to know their metabolic capabilities and to have genomic resources. In this context, the main objective of this research was to determine the genome features and the biochemical profile of Synechococcus sp. UCP002. The cyanobacterium was isolated from the Peruvian Amazon Basin region and cultured in BG-11 medium. Growth parameters, genome features, and the biochemical profile of the cyanobacterium were determined using standardized methods. Synechococcus sp. UCP002 had a specific growth rate of 0.086 ± 0.008 μ and a doubling time of 8.08 ± 0.78 h. The complete genome of Synechococcus sp. UCP002 had a size of ∼3.53 Mb with a high coverage (∼200x), and its quality parameters were acceptable (completeness = 99.29%, complete and single-copy genes = 97.5%, and contamination = 0.35%). Additionally, the cyanobacterium had six plasmids ranging from 24 to 200 kbp. The annotated genome revealed ∼3,422 genes, ∼ 3,374 protein-coding genes (with ∼41.31% hypothetical protein-coding genes), two CRISPR Cas systems, and 61 non-coding RNAs. Both the genome and plasmids had the genes for prokaryotic defense systems. Additionally, the genome had genes coding the transcription factors of the metalloregulator ArsR/SmtB family, involved in sensing heavy metal pollution. The biochemical profile showed primary nutrients, essential amino acids, some essential fatty acids, pigments (e.g., all-trans-β-carotene, chlorophyll a, and phycocyanin), and phenolic compounds. In conclusion, Synechococcus sp. UCP002 shows biotechnological potential to produce human and animal nutrients and raw materials for biofuels and could be a new source of genes for synthetic biological applications.

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Section: Resultsmentioning

confidence: 99%

Genomic analysis and biochemical profiling of an unaxenic strain of Synechococcus sp. isolated from the Peruvian Amazon Basin region

et al. 2022

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“…Here, we describe several metabolic engineering approaches adopted in cyanobacteria for increasing the production of fatty acids, carbohydrates and terpenoids. Industrially, the alkyl monoesters of fatty acids represent the starting material for biodiesel [ 225 ]; sugars such as glycogen may be used as a feedstock for bioethanol production [ 226 , 227 ] and terpenoids have broad industrial applications, from medical and agricultural to flavor and the fragrance industry [ 228 , 229 ].…”

Section: Metabolic Engineering Approaches For Increasing Cyanobacteri...mentioning

confidence: 99%

Current Metabolic Engineering Strategies for Photosynthetic Bioproduction in Cyanobacteria

2023

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Cyanobacteria are photosynthetic microorganisms capable of using solar energy to convert CO2 and H2O into O2 and energy-rich organic compounds, thus enabling sustainable production of a wide range of bio-products. More and more strains of cyanobacteria are identified that show great promise as cell platforms for the generation of bioproducts. However, strain development is still required to optimize their biosynthesis and increase titers for industrial applications. This review describes the most well-known, newest and most promising strains available to the community and gives an overview of current cyanobacterial biotechnology and the latest innovative strategies used for engineering cyanobacteria. We summarize advanced synthetic biology tools for modulating gene expression and their use in metabolic pathway engineering to increase the production of value-added compounds, such as terpenoids, fatty acids and sugars, to provide a go-to source for scientists starting research in cyanobacterial metabolic engineering.

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“…A photosynthetic mechanism involving water oxidation can lead to large-scale fuel production either by an artificial photosynthetic process (Inganäs and Sundström, 2016 ) or by directly opting for methods of solar biofuel production that is a much cleaner source of energy. This will not just take up hydrogen production but will certainly minimize the generation of reduced carbon-based biofuels by concomitant atmospheric CO 2 fixation and by designing synthetic pathways for enhanced biofuel outputs (Mund et al, 2022 ; Sebesta et al, 2022 ). An approach toward designer cyanobacterial strains with edited metabolic pathways can troubleshoot many environmental concerns at the industrial level for biofuel production.…”

Section: Cyanobacteria: a Sustainable Biofactory For Biofuel And Biop...mentioning

confidence: 99%

Cyanobacteria as a Promising Alternative for Sustainable Environment: Synthesis of Biofuel and Biodegradable Plastics

et al. 2022

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With the aim to alleviate the increasing plastic burden and carbon footprint on Earth, the role of certain microbes that are capable of capturing and sequestering excess carbon dioxide (CO2) generated by various anthropogenic means was studied. Cyanobacteria, which are photosynthetic prokaryotes, are promising alternative for carbon sequestration as well as biofuel and bioplastic production because of their minimal growth requirements, higher efficiency of photosynthesis and growth rates, presence of considerable amounts of lipids in thylakoid membranes, and cosmopolitan nature. These microbes could prove beneficial to future generations in achieving sustainable environmental goals. Their role in the production of polyhydroxyalkanoates (PHAs) as a source of intracellular energy and carbon sink is being utilized for bioplastic production. PHAs have emerged as well-suited alternatives for conventional plastics and are a parallel competitor to petrochemical-based plastics. Although a lot of studies have been conducted where plants and crops are used as sources of energy and bioplastics, cyanobacteria have been reported to have a more efficient photosynthetic process strongly responsible for increased production with limited land input along with an acceptable cost. The biodiesel production from cyanobacteria is an unconventional choice for a sustainable future as it curtails toxic sulfur release and checks the addition of aromatic hydrocarbons having efficient oxygen content, with promising combustion potential, thus making them a better choice. Here, we aim at reporting the application of cyanobacteria for biofuel production and their competent biotechnological potential, along with achievements and constraints in its pathway toward commercial benefits. This review article also highlights the role of various cyanobacterial species that are a source of green and clean energy along with their high potential in the production of biodegradable plastics.

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Advances in metabolic engineering of cyanobacteria for production of biofuels

Cited by 22 publications

References 98 publications

Genomic analysis and biochemical profiling of an unaxenic strain of Synechococcus sp. isolated from the Peruvian Amazon Basin region

Genomic analysis and biochemical profiling of an unaxenic strain of Synechococcus sp. isolated from the Peruvian Amazon Basin region

Current Metabolic Engineering Strategies for Photosynthetic Bioproduction in Cyanobacteria

Cyanobacteria as a Promising Alternative for Sustainable Environment: Synthesis of Biofuel and Biodegradable Plastics

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