Advances in mast cell biology: new understanding of heterogeneity and function

Moon, Tae Chul; Laurent, Chris D. St.; Morris, Katherine; Marcet, Candy Tsang; Yoshimura, Tsuyoshi; Sekar, Yokananth; Befus, A. Dean

doi:10.1038/mi.2009.136

Cited by 235 publications

(230 citation statements)

References 272 publications

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“…Among these cells, mast cells play a fundamental role in the immune response and tissue repair. The cytoplasm of mast cells contains a broad spectrum of pro-inflammatory mediators, and immunoregulatory and angiogenic molecules that participate in inflammatory reactions as well as tissue protection and repair 8,9 .…”

Section: Introductionmentioning

confidence: 99%

Effect of laser therapy on the inflammatory response induced by endodontic medications implanted into the subcutaneous tissue of rats

Matos

Soares

Júnior

et al. 2014

Rev. odontol. UNESP

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ResumoIntrodução: Medicamentos endodônticos apresentam componentes tóxicos que provocam algum grau de reação inflamatória. Objetivo: Este estudo avaliou o efeito da laserterapia na resposta inflamatória causada por medicações intracanais, em tecido subcutâneo de ratos, por meio da análise quantitativa de mastócitos. Material e método: Tubos de polietileno contendo as medicações foram implantados no dorso de 60 ratos, distribuídos em seis grupos: HS (pasta de hidróxido de cálcio P.A.); HL (pasta de hidróxido de cálcio P.A. e laserterapia); HPS (pasta de hidróxido de cálcio P.A. com paramonoclorofenol canforado); HPL (pasta de hidróxido de cálcio P.A. com paramonoclorofenol canforado e laserterapia); IS (iodofórmio e soro fisiológico) e IL (iodofórmio, soro fisiológico e laserterapia). Os animais foram eutanasiados oito e quinze dias após a cirurgia, as peças cirúrgicas foram removidas, processadas para inclusão em parafina e os cortes histológicos corados em Azul de Toluidina 0.2%, para quantificação dos mastócitos. A análise de variância (ANOVA) e o teste de Tukey post hoc foram aplicados para determinar diferenças significativas entre os grupos quanto ao número de mastócitos (p<0.05). Resultado: Nos grupos HL, HPL e IL houve uma diminuição de mastócitos em ambos os períodos experimentais em relação aos grupos HS, HPS e IS, porém não se observou diferença estatística significativa entre o grupo HPS e o HPL aos oito dias. Conclusão: A laserterapia foi eficaz em modular a intensidade da resposta inflamatória induzida pelos medicamentos endodônticos a partir da redução significativa na quantidade de mastócitos.Descritores: Endodontia; laserterapia; mastócitos; inflamação; subcutâneo. Abstract Introduction: Endodontic medications contain toxic components that cause varying degrees of inflammation.Objective: This study evaluated the effect of laser therapy on the inflammatory response induced by intracanal medications implanted into the subcutaneous tissue of rats using a quantitative analysis of mast cells. Material and method: Polyethylene tubes containing the medications were implanted in the dorsum of 60 rats divided into six groups, including HS (P.A. calcium hydroxide paste), HL (P.A. calcium hydroxide paste and laser therapy), HPS (P.A. calcium hydroxide paste with camphorated paramonochlorophenol), HPL (P.A. calcium hydroxide paste with camphorated paramonochlorophenol and laser therapy), IS (iodoform with saline) and IL (iodoform with saline and laser therapy). The animals were euthanized eight or fifteen days after surgery, and samples were removed and embedded in paraffin. Histological sections were stained with 0.2% toluidine blue for the quantification of mast cells. Analysis of variance (ANOVA) and Tukey's post-hoc test were applied to determine significant differences in the number of mast cells between groups (p<0.05). Result: There was a decrease in mast cells for the HL, HPL and IL groups when compared with the HS, HPS and IS groups at both time points. There was no statistically significant differe...

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Section: Introductionmentioning

confidence: 99%

Effect of laser therapy on the inflammatory response induced by endodontic medications implanted into the subcutaneous tissue of rats

Matos

Soares

Júnior

et al. 2014

Rev. odontol. UNESP

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“…It has been proposed that this splanchnic inflammatory response associated with ascites could be mediated, among others factors, by mast cells. Degranulation of splanchnic mast cells, including the mast cells present in the peritoneum, could result in the release of mediators with a potent vasodilator and exudative actions such as histamine, proteases and other enzymes, arachidonic acid metabolites and reactive oxygen and nitrogen species [76] which could cause intense exudation related to an endothelial permeability increase, which could be in turn the cause of swelling, increased splanchnic lymphatic flow and production of peritoneal exudate [9].…”

Section: Obstructive Cholestasis In the Rat: A Model Of Decompensatedmentioning

confidence: 99%

“…Mast cell heterogeneity is presumably a result of micro environmental conditions that dictate gene expression and phenotype development [76]. Given the evolutive nature of portal hypertension, the splanchnic mast cell response could be influenced by the predominant phenotype in each evolutive phase.…”

Section: The Angiogenic Phenotype: Remodeling Through Extraembryonic mentioning

confidence: 99%

Portal hypertension-related inflammatory phenotypes: From a vitelline and amniotic point of view

Aller¹,

Arias²,

Prieto³

et al. 2012

ABB

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Prehepatic portal hypertension induces a splanchnic low-grade inflammatory response that could switch to high-grade inflammation with the development of severe and life-threatening complications when associated with chronic liver disease. The extraembryonic origin of the portal system maybe determines the regression to an extraembryonic phenotype, i.e., vitellogenic and amniotic, during the evolution of both types of portal hypertension. Thus, prehepatic portal hypertension, or compensated hypertension by portal vein ligation in the rat, is associated with molecular mechanisms related to vitellogenesis, where hepatic steatosis and splanchnic angiogenesis stand out. In turn, extrahepatic cholestasis in the rat induces intrahepatic portal hypertension, or decompensated hypertension, with ascites and hepatorenal syndrome. The splanchnic interstitium, the mesenteric lymphatic system, and the peritoneal mesothelium seem to create an inflammatory pathway that could have a key pathophysiological relevance in the production of ascites. The hypothetical comparison between the ascitic and the amniotic fluid also allows for translational investtigation. The induced regression of the splanchnic system to extraembryonic functions by portal hypertension highlights the great relevance of the extraembryonic structures even during post-natal life.

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“…MCs are often sub-classified based on their "baseline" phenotypic characteristics and their anatomic locations. In mice, two types of MCs have been described: "connective tissue-type" MCs (CTMCs) and mucosal MCs (MMCs) [1][2][3]7,8 . CTMCs are often located around venules and near nerve fibers, and reside in serosal cavities, while MMCs occupy intraepithelial locations in the gut and respiratory mucosa [1][2][3] .…”

Section: Introductionmentioning

confidence: 99%

Analyzing the Functions of Mast Cells <em>In Vivo</em> Using '<em>Mast Cell Knock-in</em>' Mice

Gaudenzio

Sibilano

Starkl

et al. 2015

JoVE

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Mast cells (MCs) are hematopoietic cells which reside in various tissues, and are especially abundant at sites exposed to the external environment, such as skin, airways and gastrointestinal tract. Best known for their detrimental role in IgE-dependent allergic reactions, MCs have also emerged as important players in host defense against venom and invading bacteria and parasites. MC phenotype and function can be influenced by microenvironmental factors that may differ according to anatomic location and/or based on the type or stage of development of immune responses. For this reason, we and others have favored in vivo approaches over in vitro methods to gain insight into MC functions. Here, we describe methods for the generation of mouse bone marrow-derived cultured MCs (BMCMCs), their adoptive transfer into genetically MC-deficient mice, and the analysis of the numbers and distribution of adoptively transferred MCs at different anatomical sites. This method, named the 'mast cell knock-in' approach, has been extensively used over the past 30 years to assess the functions of MCs and MC-derived products in vivo. We discuss the advantages and limitations of this method, in light of alternative approaches that have been developed in recent years. Video LinkThe video component of this article can be found at

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Advances in mast cell biology: new understanding of heterogeneity and function

Cited by 235 publications

References 272 publications

Effect of laser therapy on the inflammatory response induced by endodontic medications implanted into the subcutaneous tissue of rats

Effect of laser therapy on the inflammatory response induced by endodontic medications implanted into the subcutaneous tissue of rats

Portal hypertension-related inflammatory phenotypes: From a vitelline and amniotic point of view

Analyzing the Functions of Mast Cells <em>In Vivo</em> Using '<em>Mast Cell Knock-in</em>' Mice

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