Advances in Integrated Genetic Analysis

Anderson, Rolfe C.; Bogdan, Gregory J.; Puski, Alex; Su, Xing

doi:10.1007/978-94-011-5286-0_3

Cited by 17 publications

(7 citation statements)

References 6 publications

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“…In general, microfluidic mixing is dominated by diffusion because of a low Reynolds number . To overcome this notorious “mixing problem,” various micromixers have been devised for continuous-flow systems. − On the basis of classic studies, − it has been recently discussed that such mixers are unnecessary for segmented-flow systems because recirculation in a moving droplet (Figure ) has a stirring effect 5 Video-clipped fluorescence images of handling operations for two fluorescein aqueous solutions with different concentrations.…”

Section: Resultsmentioning

confidence: 99%

Handling of Picoliter Liquid Samples in a Poly(dimethylsiloxane)-Based Microfluidic Device

1999

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Transportation, metering, and mixing of picoliter-sized liquid samples were realized in a microfluidic device with a main working area of one square millimeter. The device was constructed by sealing microfabricated grooves on a chip made of poly(dimethylsiloxane) (PDMS). Two different samples were segmented into 600-pL droplets in a microchannel with a cross section of W (100 μm) × H (25 μm), and the droplets were merged together. For acceleration of the mixing, the merged droplet was shuttled back and forth. Recirculation in a moving droplet was proven to be effective for high-speed mixing in this diffusion-dominated scale. All the handling operations were carried out using air pressure transferred through microfabricated vent valves which have been newly developed. The demonstrated strategy, including fabrication, leads to high-performance and low-cost micro total analysis systems (μTAS).

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Section: Resultsmentioning

confidence: 99%

Handling of Picoliter Liquid Samples in a Poly(dimethylsiloxane)-Based Microfluidic Device

1999

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“…Moreover the recirculation streamlines of liquid in a moving discrete drop [17] allows convective mixing of solutes in addition to molecular diffusion. This recirculation effect has been alluded to in the works of Anderson et al [14,15], Burns et al [10,11], Sammarco [12] and Hosokowa et al [16], and mixing experiments of low molecular weight solutes (diffusivity on the order of 10 −5 cm 2 s −1 ) have been reported [15,16].…”

Section: Introductionmentioning

confidence: 84%

Mathematical modeling of drop mixing in a slit-type microchannel

Handique¹,

Burns

2001

J. Micromech. Microeng.

110

129

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Fast solute mixing can be achieved in a microchannel by rapid unidirectional displacement of a discrete liquid drop. The recirculation streamlines created within the liquid during the drop's motion cause the solute to interlayer across the channel depth, provided the interlayer diffusion of the solute is small. Uniform interlayering appears when the drop is displaced by more than three drop lengths in a slit-type microchannel, thereby reducing the solute diffusion distances to a fraction of the channel depth. By fabricating the microchannel to a depth of less than 50 µm even large molecules with a low diffusivity (D < 10 −8 cm 2 s −1) can be mixed in seconds. The above strategy is shown by modeling the mixing of solutes present in a drop moving in a slit-type microchannel.

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“…For example, Orchid Biocomputer uses capillary valves and pressure to control fluid deliveries and well volume consistency across multiple reactor wells in combinatorial synthesis (DeWitt, 1999). In preparing fluids for its "genechips," Affymetrix uses surface tension differences along fluid channels to precisely meter fluids and pressure to move the resulting fluid packets around on the microfluidic chip (Anderson et al, 1998). Strategically placed permeable gas membranes allow gas to pass through but stop the fluids at specific locations.…”

Section: Discussionmentioning

confidence: 99%