Handling of Picoliter Liquid Samples in a Poly(dimethylsiloxane)-Based Microfluidic Device

Hosokawa, Kazuo; Fujii, Teruo; Endo, Itaru

doi:10.1021/ac990571d

Cited by 241 publications

(216 citation statements)

References 27 publications

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“…The scaling relation (eqn (1)) is independent of the material parameters of the fluids-their viscosities and the interfacial tension between them. This effect is specific to microsystems: at rates of flow (10 22 to 1 mL s 21 ) that are typically used for flow in microchannels (having characteristic dimensions on the order of 100 mm), the capillary numbers (Ca = mu/c, where m is the viscosity, and u is the mean speed of the carrier fluid, and c is the interfacial tension) are typically small (Ca , 10…”

Section: Introductionmentioning

confidence: 99%

Formation of droplets and bubbles in a microfluidic T-junction—scaling and mechanism of break-up

et al. 2006

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This article describes the process of formation of droplets and bubbles in microfluidic T-junction geometries. At low capillary numbers break-up is not dominated by shear stresses: experimental results support the assertion that the dominant contribution to the dynamics of break-up arises from the pressure drop across the emerging droplet or bubble. This pressure drop results from the high resistance to flow of the continuous (carrier) fluid in the thin films that separate the droplet from the walls of the microchannel when the droplet fills almost the entire cross-section of the channel. A simple scaling relation, based on this assertion, predicts the size of droplets and bubbles produced in the T-junctions over a range of rates of flow of the two immiscible phases, the viscosity of the continuous phase, the interfacial tension, and the geometrical dimensions of the device.

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Section: Introductionmentioning

confidence: 99%

Formation of droplets and bubbles in a microfluidic T-junction—scaling and mechanism of break-up

et al. 2006

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“…5,6 In this paper we study the dynamics of a threedimensional thin film spreading on a dry inclined substrate, where the contact line is gravity driven, as shown in Fig. 1.…”

Section: Introductionmentioning

confidence: 99%

Dynamics of driven three-dimensional thin films: From hydrophilic to superhydrophobic regimes

2008

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We study the forced displacement of a thin film of fluid in contact with vertical and inclined substrates of different wetting properties, that range from hydrophilic to hydrophobic, using the lattice-Boltzmann method. We study the stability and pattern formation of the contact line in the hydrophilic and superhydrophobic regimes, which correspond to wedge-shaped and nose-shaped fronts, respectively. We find that contact lines are considerably more stable for hydrophilic substrates and small inclination angles. The qualitative behavior of the front in the linear regime remains independent of the wetting properties of the substrate as a single dispersion relation describes the stability of both wedges and noses. Nonlinear patterns show a clear dependence on wetting properties and substrate inclination angle. The effect is quantified in terms of the pattern growth rate, which vanishes for the sawtooth pattern and is finite for the finger pattern. Sawtooth shaped patterns are observed for hydrophilic substrates and low inclination angles, while finger-shaped patterns arise for hydrophobic substrates and large inclination angles. Finger dynamics show a transient in which neighboring fingers interact, followed by a steady state where each finger grows independently.

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“…Because each droplet can be independently controlled, highly integrated, scalable and flexible architectures can be implemented. 10 A number of techniques have been described for the actuation of droplets on solid surfaces including the use of thermocapillary effects, 14 photochemical effects, 15 electrochemical gradients, 16 surface tension gradients, 17 temperature gradients, 18 air pressure, 19 structured surfaces, 20 dielectrophoresis, 21 and electrostatic methods. 8 An extension of this approach is a liquid-liquid microfluidic system for manipulating freely suspended microliter or nanoliter droplets.…”

Section: Introductionmentioning

confidence: 99%

Local Heating of Discrete Droplets Using Magnetic Porous Silicon-Based Photonic Crystals

et al. 2006

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This paper describes a method for local heating of discrete micro-liter scale liquid droplets. The droplets are covered with magnetic porous Si microparticles, and heating is achieved by application of an external alternating electromagnetic field. The magnetic porous Si microparticles consist of two layers: the top layer contains a photonic code and it is hydrophobic, with surfacegrafted dodecyl moieties. The bottom layer consists of a hydrophilic Si oxide host layer that is infused with Fe 3 O 4 nanoparticles. The amphiphilic microparticles spontaneously align at the interface of a water droplet immersed in mineral oil, allowing manipulation of the droplets by application of a magnetic field. Application of an oscillating magnetic field (338 kHz, 18A RMS current in a coil surrounding the experiment) generates heat in the superparamagnetic particles that can raise the temperature of the enclosed water droplet to >80 °C within 5 min.A simple microfluidics application is demonstrated: combining complementary DNA strands contained in separate droplets and then thermally inducing dehybridization of the conjugate. The complementary oligonucleotides were conjugated with the cyanine dye fluorophores Cy3 and Cy5 to quantify the melting/re-binding reaction by fluorescence resonance energy transfer (FRET). The magnetic porous Si microparticles were prepared as photonic crystals, containing spectral codes that allowed the identification of the droplets by reflectivity spectroscopy. The technique demonstrates the feasibility of tagging, manipulating, and heating small volumes of liquids without the use of conventional microfluidic channel and heating systems.

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Handling of Picoliter Liquid Samples in a Poly(dimethylsiloxane)-Based Microfluidic Device

Cited by 241 publications

References 27 publications

Formation of droplets and bubbles in a microfluidic T-junction—scaling and mechanism of break-up

Formation of droplets and bubbles in a microfluidic T-junction—scaling and mechanism of break-up

Dynamics of driven three-dimensional thin films: From hydrophilic to superhydrophobic regimes

Local Heating of Discrete Droplets Using Magnetic Porous Silicon-Based Photonic Crystals

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