Advances in immunopharmacology of asthma

Wong, W.S. Fred; Koh, Dow-Rhoon

doi:10.1016/s0006-2952(99)00378-0

Cited by 38 publications

(37 citation statements)

References 120 publications

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“…eosinophils, T cells, mast cells) and structural cells (airway epithelial cells, smooth muscle cells, endothelial cells, fibroblasts) 1,2 . The inflammation causes an associated increase in airway responsiveness to various stimuli 3 .…”

Section: Introductionmentioning

confidence: 99%

Mechanistic Evaluation of Butea Monosperma Using in Vitro and in Vivo Murine Models of Bronchial Asthma

Shirole¹,

Sutariya²,

Saraf³

2013

Int. J. Res. Ayurveda Pharm.

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The present investigation aimed to elucidate the probable mechanism of antiasthmatic action of Butea monosperma. Bioactive fractionations of methanolic extract of B. monosperma (MBM) were carried out to get, ethyl acetate fraction (EBM) and butanolic fraction (BBM) of Butea monosperma. All fractions were evaluated mechanistically for its antiasthmatic actions in vitro using lipoxygenase assay. The lipoxygenase inhibitory effect of the extract followed the order BBM > MBM > EBM. Based on in vitro lipoxygenase assay BBM was selected for in vitro compound 48/80 induced mast cell degranulation assay and in vivo lipopolysaccharide induced inflammation in rats. BBM 50, 100 and 200μg/ml inhibited compound 48/80 induced mast cell degranulation. BBM exhibited dose dependent (50, 100 and 200mg/kg i.p.) inhibition of lipopolysaccharide-induced increased in total cell count, differential leukocyte count, nitrate-nitrite, total protein and albumin levels in bronchoalveolar fluid (BALF) and myeloperoxidase (MPO) levels in lungs homogenate.

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Section: Introductionmentioning

confidence: 99%

Mechanistic Evaluation of Butea Monosperma Using in Vitro and in Vivo Murine Models of Bronchial Asthma

Shirole¹,

Sutariya²,

Saraf³

2013

Int. J. Res. Ayurveda Pharm.

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“…PMA and ionomycin and/ or IL-4 were employed to induce the phosphorylation of STAT6. PMA activates protein kinase C (PKC) [40] and ionomycin increases the Ca 2+ influx [41]. Ca 2+ influx is also dependent on the PKC mediated pathways for the production of IL-4 [42].…”

Section: Discussionmentioning

confidence: 99%

Histamine affects STAT6 phosphorylation via its effects on IL-4 secretion: Role of H1 receptors in the regulation of IL-4 production

Kharmate

Liu

Patterson

et al. 2007

International Immunopharmacology

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Signal Transducer and Activator of Transcription (STAT)-6 is a transcriptional factor activated mainly through the cytokines IL-4 and IL-13 leading to the Th2 cell differentiation. Th2 cells play a role in the etiology and pathogenesis of allergic disease. Histamine alters the Th1/Th2 cytokine balance towards the Th2 cytokine profile and consequently plays a role in the allergic diseases and asthma. This study was designed to investigate the effects of histamine on the STAT6 phosphorylation. C57/BL6 splenocytes were pre-treated with different concentrations of histamine (10 −4 M to 10 −13 M) followed by stimulation with PMA + ionomycin or IL-4. The phosphorylated and total basal STAT6 levels were assessed by employing the immunoblotting technique. Histamine caused the hyper-phosphorylation of STAT-6. H1 receptor antagonist pyrilamine reversed the effect of histamine on STAT6 phosphorylation. However, H2 receptor antagonist ranitidine and H3/H4 receptor antagonist thioperamide did not affect the histamine mediated hyper-phosphorylation of STAT6. Furthermore, H1 receptor agonist betahistine enhanced the phosphorylation of STAT6 whereas H2 receptor agonist amthamine did not affect the phosphorylation STAT6. Furthermore, tyrosine kinase inhibitor, tyrphostin, inhibited the histamine mediated phosphorylation of STAT6 when stimulated with PMA + ionomycin. The effects of histamine on the STAT6 phosphorylation were indirect since they were blocked either by the antibodies to IL-4 and IL-13 or in IL-4 knock out mice in the presence of IL-13 antibody. These observations suggest that histamine indirectly affected the STAT6 phosphorylation via its effects on the secretion of cytokines (IL-4) and H1 receptor played a role in this process.

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“…Cissampelos sympodialis on the immunoglobulin production B cells are responsible for the production of immunoglobulins (Ig) after antigen recognition and activation (Snapper & Paul, 1987;Wong & Koh, 2000). Asthma reaction is an immediatetype reaction mediated mainly by IgE (Busse & Lemanski, 2001;Mayr, et al, 2003).…”

Section: Effect Ofmentioning

confidence: 99%

“…Also it was demonstrated that cAMP is a second messenger that plays an important role in the regulation of B cell apoptosis (Myklebust et al, 1999). In general, an increase in cAMP levels is associated with anti-inflammatory and immunosuppressive effects (Cohen & Rothstein, 1989;Wong & Koh, 2000;Torgersen et al, 2002). Finally, the finding that AFL inhibited immunoglobulin secretion suggests a therapeutic use for the Cissampelos sympodialis extract in conditions associated with up regulation of B cell function and enhanced immunoglobulin secretion such as allergic diseases as well as autoimmune disease.…”

Section: Effect Ofmentioning

confidence: 99%

Cissampelos sympodialis (Menispermaceae): A Novel Phytotherapic Weapon Against Allergic Diseases?

Piuvezam¹,

Bezerra-Santos²,

Bozza³

et al. 2012

Allergic Diseases - Highlights in the Clinic, Mechanisms and Treatment

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Advances in immunopharmacology of asthma

Cited by 38 publications

References 120 publications

Mechanistic Evaluation of Butea Monosperma Using in Vitro and in Vivo Murine Models of Bronchial Asthma

Mechanistic Evaluation of Butea Monosperma Using in Vitro and in Vivo Murine Models of Bronchial Asthma

Histamine affects STAT6 phosphorylation via its effects on IL-4 secretion: Role of H1 receptors in the regulation of IL-4 production

Cissampelos sympodialis (Menispermaceae): A Novel Phytotherapic Weapon Against Allergic Diseases?

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