Advances in fish vaccine delivery

Plant, Karen P.; LaPatra, Scott E.

doi:10.1016/j.dci.2011.03.007

Cited by 309 publications

(173 citation statements)

References 67 publications

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“…In this scenario, controlling disease outbreaks by vaccination and the use of immune modulator enriched diets represents a major achievement for aquaculture species. Although fish vaccination is still crawling, it has been successfully used to control important fish diseases (Sommerset et al 2005, Secombes 2008, Van Muiswinkel 2008, Plant & Lapatra 2011. And, more recently, vaccination commingled with the inclusion of immune-modulator molecules in the diet offered and additional strategy to overcome major pathogen (Newaj-Fyzul & Austin 2015).…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory effects of dietary β-glucan in silver catfish (Rhamdia quelen)

Domenico

Canova²,

Soveral³

et al. 2017

Pesq. Vet. Bras.

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RESUMO.-[Efeito imunomodulador da β-glucana usada como aditivo alimentar em jundiás (Rhamdia quelen).]O uso da β-glucana como suplemento alimentar foi avaliado em jundiás. A β-glucana foi adicionada à ração na proporção de 0.01%, e 0.1% e fornecida aos peixes por 21, para avaliar dados hematológicos e parâmetros do sistema imune natural, ou 42 dias, para avaliar ganho de peso e resistência ao desafio com Aeromonas hydrophila. A adição da β-glucana na dieta não afetou o ganho de peso e não induziu alterações hematológicas nem alterações nos níveis de aglutininas e mieloperoxidase sanguínea. No entanto, a atividade hemolítica natural do sistema do complemento foi significativamente maior nos peixes alimentados com β-glucana. Além disso, nos peixes alimentados com β-glucana e desafiados com A. hydrophila, o número de bactérias isoladas do sangue foi significativamente menor, e a sobrevivência ao desafio foi significativamente maior do que nos peixes que não receberam β-glucana. Consequentemente, concluímos que a β-glucana tem potencial imunomodulador quando adicionada à dieta, nas condições experimentais aqui indicadas, e contribui para aumentar imunidade natural e a resistência dos jundiás ao desafio com patógenos específicos. INTRODUCTIONThe occurrence of infectious diseases is one of the major causes of losses in modern aquaculture (Sitjà-Bobadilla The immunomodulatory effects of dietary β-glucan were evaluated in silver catfish. β-glucan was added to the diet (0.01%, and 0.1%) and fed to the fish for 21 days, to evaluate effects on blood and some innate immune parameter, or fed for 42 days, to evaluate growth rate and resistance to challenge with pathogenic Aeromonas hydrophila. We found that adding β-glucan to the diet had no effect on fish growth and no effect on blood cells, or serum bacterial agglutination and serum myeloperoxidase activity. However, fish that received β-glucan in the diet had the natural hemolytic activity of complement significantly higher compared to control fish. Furthermore, fish fed with β-glucan and challenged with A. hydrophila had fewer bacteria in blood and presented a significantly higher survival rate compared to control fish. Thus, we concluded that β-glucan might be explored as feed additive aiming to improve silver catfish innate immunity and resistance to specific pathogen.INDEX TERMS: Silver catfish, Rhamdia quelen, fish, immunostimulants, β-glucan, Aeromonas hydrophila.

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Section: Discussionmentioning

confidence: 99%

Immunomodulatory effects of dietary β-glucan in silver catfish (Rhamdia quelen)

Domenico

Canova²,

Soveral³

et al. 2017

Pesq. Vet. Bras.

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“…Currently, there is a need for effective orally delivered vaccines (90). Thus, studies about fish gut-bacterial interaction become important to develop effective oral vaccines for aquaculture.…”

Section: Fig 8 E Ictaluri Colonization Ratio In Intestinal Loopmentioning

confidence: 99%

Inflammatory Effects of Edwardsiella ictaluri Lipopolysaccharide Modifications in Catfish Gut

et al. 2014

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e Bacterial lipopolysaccharides (LPS) are structural components of the outer membranes of Gram-negative bacteria and also are potent inducers of inflammation in mammals. Higher vertebrates are extremely sensitive to LPS, but lower vertebrates, like fish, are resistant to their systemic toxic effects. However, the effects of LPS on the fish intestinal mucosa remain unknown. Edwardsiella ictaluri is a primitive member of the Enterobacteriaceae family that causes enteric septicemia in channel catfish (Ictalurus punctatus). E. ictaluri infects and colonizes deep lymphoid tissues upon oral or immersion infection. Both gut and olfactory organs are the primary sites of invasion. At the systemic level, E. ictaluri pathogenesis is relatively well characterized, but our knowledge about E. ictaluri intestinal interaction is limited. Recently, we observed that E. ictaluri oligo-polysaccharide (O-PS) LPS mutants have differential effects on the intestinal epithelia of orally inoculated catfish. Here we evaluate the effects of E. ictaluri O-PS LPS mutants by using a novel catfish intestinal loop model and compare it to the rabbit ileal loop model inoculated with Salmonella enterica serovar Typhimurium LPS. We found evident differences in rabbit ileal loop and catfish ileal loop responses to E. ictaluri and S. Typhimurium LPS. We determined that catfish respond to E. ictaluri LPS but not to S. Typhimurium LPS. We also determined that E. ictaluri inhibits cytokine production and induces disruption of the intestinal fish epithelia in an O-PS-dependent fashion. The E. ictaluri wild type and ⌬wibT LPS mutant caused intestinal tissue damage and inhibited proinflammatory cytokine synthesis, in contrast to E. ictaluri ⌬gne and ⌬ugd LPS mutants. We concluded that the E. ictaluri O-PS subunits play a major role during pathogenesis, since they influence the recognition of the LPS by the intestinal mucosal immune system of the catfish. The LPS structure of E. ictaluri mutants is needed to understand the mechanism of interaction.

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“…Currently, defense against microorganisms can be stimulated through immunization and the use of immunostimulants, with consequent increase of circulating antibodies concentration, survival and production performance (POUEY et al, 2011;SUHET et al, 2011;TIZARD, 2002). In aquaculture, immunization can be an alternative to antibiotics, since disease prevention is critical to the development of the activity (ROMANO & MEJÍA, 2003;PLANT & LAPATRA, 2011).…”

Section: Resultsmentioning

confidence: 99%

HEMAGGLUTINATION ANTIBODY TITERS IN PACU, Piaractus mesopotamicus, AS AN INDICATOR OF ACQUIRED IMMUNITY/Título de anticorpos hemaglutinantes de Pacu, Piaractus mesopotamicus, como indicador de imunidade adquirida

Takahashi

Urbinati

2013

Ars Vet

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SUMMARYThe evaluation of the hemagglutination titer is an option to evaluate the response of acquired immune system in order to assess the immunocompetence for antibodies production. The study was carried out to standardize the antibody titer against rabbit red blood cell in immunized fish and fed diets with levamisole (0; 250 and 500 mg.kg -1 levamisole). As a result, the cell cluster agglutination can be observed by the naked eye. Fish fed 250 mg.kg -1 of levamisole have shown the highest hemagglutination antibodies titer; however, fish fed 500 mg.kg -1 of levamisole have revealed titers equivalent to control group that was fed levamisole-free diet. This study has validated the methodology for determination of hemagglutination antibody titer of immunized fish and has found that antibody titers increased after feeding a diet containing 250 mg.kg -1 of levamisole during 10 days.

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Advances in fish vaccine delivery

Cited by 309 publications

References 67 publications

Immunomodulatory effects of dietary β-glucan in silver catfish (Rhamdia quelen)

Immunomodulatory effects of dietary β-glucan in silver catfish (Rhamdia quelen)

Inflammatory Effects of Edwardsiella ictaluri Lipopolysaccharide Modifications in Catfish Gut

HEMAGGLUTINATION ANTIBODY TITERS IN PACU, Piaractus mesopotamicus, AS AN INDICATOR OF ACQUIRED IMMUNITY/Título de anticorpos hemaglutinantes de Pacu, Piaractus mesopotamicus, como indicador de imunidade adquirida

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