Advances in chloroplast engineering

Wang, Huanhuan; Yin, Wei-Bo; Hu, Zhongliang

doi:10.1016/s1673-8527(08)60128-9

Cited by 64 publications

(66 citation statements)

References 99 publications

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“…Several advantages such as accumulation of high levels of foreign proteins, the feasibility of expressing multiple proteins from polycistronic mRNAs, gene containment through the lack of plastids in mature pollens, biosafety and absence of epigenetic transgene instability effects are some of the chloroplast engineering profits. Considering these advantages, induction of transplastomic plants could be useful for conferring desirable agronomic traits, metabolic engineering and producing biopharmaceuticals (6)(7)(8). Chloroplast transformation was generally accomplished by the biolistic method, with the Escherichia coli vectors which contains a selectable marker gene, homologous recombination regions and the gene of interest (1,8,9).…”

Section: Introductionmentioning

confidence: 99%

“…Considering these advantages, induction of transplastomic plants could be useful for conferring desirable agronomic traits, metabolic engineering and producing biopharmaceuticals (6)(7)(8). Chloroplast transformation was generally accomplished by the biolistic method, with the Escherichia coli vectors which contains a selectable marker gene, homologous recombination regions and the gene of interest (1,8,9). This technology is established and can be routinely used in tobacco transformation; But now, successful plastid transformation was reported in other plants such as rice (Oryza sativa), soybean (Glycine max), eggplant (Solanum melongena L.), cauliflower (Brassica oleracea), cabbage (Brassica capitata), lettuce (Lactuca sativa), oilseed rape (Brassica napus), petunia (Petunia hybrid), poplar (Populus spp.…”

Section: Introductionmentioning

confidence: 99%

“…This technology is established and can be routinely used in tobacco transformation; But now, successful plastid transformation was reported in other plants such as rice (Oryza sativa), soybean (Glycine max), eggplant (Solanum melongena L.), cauliflower (Brassica oleracea), cabbage (Brassica capitata), lettuce (Lactuca sativa), oilseed rape (Brassica napus), petunia (Petunia hybrid), poplar (Populus spp. ), potato (Solanum tuberosum), tomato (Solanum lycopersicum), carrot (Daucus carota) and cotton (Gossypium hirsutum) (8,10,11).…”

Section: Introductionmentioning

confidence: 99%

“…It encodes aminoglycoside 3΄-adenylyltransferase which is responsible for both spectinomycin and streptomycin resistances. This marker is generally used to construct the chloroplast vector (8,15,19). Also 16S rRNA promoter (Prrn) and rbcL 3΄UTR are putative promoter and terminator in chloroplasts (9).…”

Section: Introductionmentioning

confidence: 99%

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Assessment of Chloroplast Expression Factors in Escherichia coli

Shahmoradi

Memari

Nekoei

et al. 2013

Jundishapur J Microbiol

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Background: Genetic manipulation of chloroplast in higher plants offers a number of unique prerogatives, including; undesirable of pleiotropic genome and gene silencing effects and also use as an important agronomic trait for producing essential biomaterials and industrial enzymes. In order to manipulate chloroplast genome, specific vectors are required. These vectors can be transformed and expressed in Escherichia coli due to the same evolutionary origin of bacteria and chloroplasts. Objectives: The aim of the present study was to construct chloroplast vector specified for spinach and assessing the chloroplast regulatory elements in a prokaryotic expression host, E. coli. Materials and Methods: Flanking sequences (INSL+INSR) were isolated by PCR from the spinach chloroplast genome and blunt-end ligated into the PvuII site of pUC19 vector to form an intermediate vector, pUCINS. Then the selectable marker cassette (including aadA gene, Prrn promoter and rbcL terminator) was isolated via PCR and blunt-end cloned into the unique PvuII site of pUCINS to make the final chloroplast vector, named pCSI. Results: The constructed vector transformed to E.coli strain DH5α and several procedures such as colony PCR, digestion and sequencing were assigned to confirm the consequence of the construct. Conclusions: The appearance of bacterial colonies on the plate containing different concentrations of streptomycin indicated the strength of resistance to streptomycin which showed the bacterial cells capability to express aadA gene under the controls of chloroplast regulatory elements.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Assessment of Chloroplast Expression Factors in Escherichia coli

Shahmoradi

Memari

Nekoei

et al. 2013

Jundishapur J Microbiol

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“…Plastid transformation is a powerful tool for producing useful proteins in plants and is also an important technique for investigating the function of genes encoded in plastid DNA (Bock 2007;Daniell et al 2002;Maliga 2003;Wang et al 2009). Extra genes integrated into plastid DNA are difficult to spread by pollen due to the maternal inheritance of plastid DNA, although low-level paternal inheritance of plastid DNA was detected in large screening of F 1 seeds obtained by artificial crossing (Ruf et al 2007).…”

mentioning

confidence: 99%

Efficient plastid transformation in tobacco using small gold particles (0.07^|^#8211;0.3^|^#8201;^|^micro;m)

Okuzaki

Kida

Watanabe

et al. 2013

Plant Biotechnology

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Plastid transformation methods have been developed for 20 plant species. However, only a few plant species, such as tobacco and lettuce, have been used in applied studies because transformation efficiencies are extremely low in other species. Plastid transformation has been mainly performed by particle bombardment using 0.6-µm gold particles as microcarriers of the transformation vector. Because the target materials in some plant species are undeveloped proplastids rather than fully developed chloroplasts, optimizing microcarrier size for the target size is a major consideration. In this study, we evaluated the availability of gold particles (0.07, 0.08, 0.1, 0.2, and 0.3 µm) that were smaller than those used for plastid transformation in previous studies. We obtained stable plastid transformants of tobacco with sufficient efficiency using all the tested small gold particles as the same level as 0.6-µm gold particles, even the smallest (0.07 µm). The average number of transformants obtained with 0.3-µm particles (9.3±4.6 per plate) was the highest among the tested gold particles. Because small gold particles were revealed to be sufficient for plastid transformation in a model tobacco plant, it is suggested that choosing appropriate small-sized gold particles which have never been used before will improve plastid transformation in many plant species.

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Biological Containment Strategies for Transgenic Crops

Maagd

Boutilier

2012

Genetically Modified and Non‐Genetically Modified Food Supply Chains: Co‐Existence and Traceability

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Advances in chloroplast engineering

Cited by 64 publications

References 99 publications

Assessment of Chloroplast Expression Factors in Escherichia coli

Assessment of Chloroplast Expression Factors in Escherichia coli

Efficient plastid transformation in tobacco using small gold particles (0.07^|^#8211;0.3^|^#8201;^|^micro;m)

Biological Containment Strategies for Transgenic Crops

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