“…The proteome changes during the muscle-to-meat conversion process are radical; many studies have been finalized to shed light on these variations [36], [37], [38], [39] and [40], and many others demonstrated the process of muscle proteolysis as the hub of muscle evolution into meat [41], [42] and [43]; our 2D results are in agreement with previous findings, as stated by variations in differential spots containing structural proteins: spots 458, 459, 466 and 471 are identified as troponin T. The time course trend indicates that an important downregulation of troponin T of longissimus thoracis Piedmontese muscle begins to appear from day 17 onward, to reach its top at day 44 (4.3 fold change for spot 458; 7.2 fold change for spot 459, Table 1); this experimental result rewards the 44 days-aged meat, because, as it has been previously reported [38] and [44], degradation of troponin T is known to be a descriptive indicator of meat tenderization process. Another component of muscular architecture, actin, clearly describes the ongoing proteolysis during the aging period; later in our discussion we will recall the upward trend of a particular actin fragment (31 kDa) linked with apoptosis.…”