Apoptosis in muscle-to-meat aging process: The omic witness

Longo, Valentina; Lana, Alessandro; Bottero, Maria Teresa; Zolla, Lello

doi:10.1016/j.jprot.2015.04.023

Cited by 49 publications

(27 citation statements)

References 82 publications

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“…Supporting this hypothesis, it was proposed that phosphorylation of PGM1 is related to faster rates of glycolysis and pH drop in postmortem muscle [74]. Furthermore, an increase in the phosphorylated PGM1 abundance was observed in cattle muscle from 0 to 1 day after slaughter, which would be related to an increase in glycogenolysis and glycolysis due to increased anaerobic postmortem muscle metabolism [75]. …”

Section: Resultsmentioning

confidence: 99%

Differences in Beef Quality between Angus (Bos taurus taurus) and Nellore (Bos taurus indicus) Cattle through a Proteomic and Phosphoproteomic Approach

et al. 2017

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Proteins are the major constituents of muscle and are key molecules regulating the metabolic changes during conversion of muscle to meat. Brazil is one of the largest exporters of beef and most Brazilian cattle are composed by zebu (Nellore) genotype. Bos indicus beef is generally leaner and tougher than Bos taurus such as Angus. The aim of this study was to compare the muscle proteomic and phosphoproteomic profile of Angus and Nellore. Seven animals of each breed previously subjected the same growth management were confined for 84 days. Proteins were extracted from Longissimus lumborum samples collected immediately after slaughter and separated by two-dimensional electrophoresis. Pro-Q Diamond stain was used in phosphoproteomics. Proteins identification was performed using matrix assisted laser desorption/ionization time-of-flight mass spectrometry. Tropomyosin alpha-1 chain, troponin-T, myosin light chain-1 fragment, cytoplasmic malate dehydrogenase, alpha-enolase and 78 kDa glucose-regulated protein were more abundant in Nellore, while myosin light chain 3, prohibitin, mitochondrial stress-70 protein and heat shock 70 kDa protein 6 were more abundant in Angus (P<0.05). Nellore had higher phosphorylation of myosin regulatory light chain-2, alpha actin-1, triosephosphate isomerase and 14-3-3 protein epsilon. However, Angus had greater phosphorylation of phosphoglucomutase-1 and troponin-T (P<0.05). Therefore, proteins involved in contraction and muscle organization, myofilaments expressed in fast or slow-twitch fibers and heat shock proteins localized in mitochondria or sarcoplasmic reticulum and involved in cell flux of calcium and apoptosis might be associated with differences in beef quality between Angus and Nellore. Furthermore, prohibitin appears to be a potential biomarker of intramuscular fat in cattle. Additionally, differences in phosphorylation of myofilaments and glycolytic enzymes could be involved with differences in muscle contraction force, susceptibility to calpain, apoptosis and postmortem glycolysis, which might also be related to differences in beef quality among Angus and Nellore.

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Section: Resultsmentioning

confidence: 99%

Differences in Beef Quality between Angus (Bos taurus taurus) and Nellore (Bos taurus indicus) Cattle through a Proteomic and Phosphoproteomic Approach

et al. 2017

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“…Большинство исследований используют 2DGE высокого разрешения или жидкостную хроматогра-фию в комбинации с масс спектрометрией (MS) для идентификации белка с высокой пропускной способ-ностью [2,3,4,5,6].…”

Section: протеомика в исследованиях биохимии мышцunclassified

“…Most studies use highresolution 2DGE or liquid chromatography, in combination with mass spectrometry (MS) for high-throughput protein identification [2,3,4,5,6].…”

Section: Proteomics In Research On Muscle Biochemistrymentioning

confidence: 99%

Proteomics in Meat Science — Current Status and Future Perspective

Zamaratskaia

2017

Teor. prakt. pererab. mâsa (Print)

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“…The findings from meat proteomics are important for maximizing muscle growth, meat production, slaughter methods, muscle-to-meat processing and meat storage [249,250,251]. …”

Section: Comparative Skeletal Muscle Proteomics Using Two-dimensiomentioning

confidence: 99%

Comparative Skeletal Muscle Proteomics Using Two-Dimensional Gel Electrophoresis

Murphy¹,

Dowling²,

Ohlendieck³

2016

Proteomes

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The pioneering work by Patrick H. O’Farrell established two-dimensional gel electrophoresis as one of the most important high-resolution protein separation techniques of modern biochemistry (Journal of Biological Chemistry 1975, 250, 4007–4021). The application of two-dimensional gel electrophoresis has played a key role in the systematic identification and detailed characterization of the protein constituents of skeletal muscles. Protein changes during myogenesis, muscle maturation, fibre type specification, physiological muscle adaptations and natural muscle aging were studied in depth by the original O’Farrell method or slightly modified gel electrophoretic techniques. Over the last 40 years, the combined usage of isoelectric focusing in the first dimension and sodium dodecyl sulfate polyacrylamide slab gel electrophoresis in the second dimension has been successfully employed in several hundred published studies on gel-based skeletal muscle biochemistry. This review focuses on normal and physiologically challenged skeletal muscle tissues and outlines key findings from mass spectrometry-based muscle proteomics, which was instrumental in the identification of several thousand individual protein isoforms following gel electrophoretic separation. These muscle-associated protein species belong to the diverse group of regulatory and contractile proteins of the acto-myosin apparatus that forms the sarcomere, cytoskeletal proteins, metabolic enzymes and transporters, signaling proteins, ion-handling proteins, molecular chaperones and extracellular matrix proteins.

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Apoptosis in muscle-to-meat aging process: The omic witness

Cited by 49 publications

References 82 publications

Differences in Beef Quality between Angus (Bos taurus taurus) and Nellore (Bos taurus indicus) Cattle through a Proteomic and Phosphoproteomic Approach

Differences in Beef Quality between Angus (Bos taurus taurus) and Nellore (Bos taurus indicus) Cattle through a Proteomic and Phosphoproteomic Approach

Proteomics in Meat Science — Current Status and Future Perspective

Comparative Skeletal Muscle Proteomics Using Two-Dimensional Gel Electrophoresis

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