Advances in and prospects of microchip liquid chromatography

Grinias, James P.; Kennedy, Robert T.

doi:10.1016/j.trac.2015.08.002

Cited by 71 publications

(49 citation statements)

References 65 publications

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“…The potential advantages of miniaturized chromatographic devices are well known and have been previously discussed in the literature (Grinias & Kennedy, 2016;Kutter, 2012). Most traditional chromatographic methods utilize cylindrical columns to take advantage of the mechanical strength and simple flow patterns for this geometry.…”

Section: Design Considerationsmentioning

confidence: 99%

“…The planar format is the dominant format for microfluidic separation devices due to the ease of its fabrication and design (Grinias & Kennedy, 2016;Kutter, 2012). The planar format is the dominant format for microfluidic separation devices due to the ease of its fabrication and design (Grinias & Kennedy, 2016;Kutter, 2012).…”

Section: Design Considerationsmentioning

confidence: 99%

“…Millet et al (2015) have investigated a modular microfluidics platform for protein purification which incorporate both affinity chromatography and size-exclusion chromatography. Another consideration is that microfluidic devices used for chromatography are potentially scalable over a broad range which increase their versatility (Grinias & Kennedy, 2016;Lazar et al, 2006;Malmstadt, Yager, Hoffman, & Stayton, 2003;Xie et al, 2005). Another consideration is that microfluidic devices used for chromatography are potentially scalable over a broad range which increase their versatility (Grinias & Kennedy, 2016;Lazar et al, 2006;Malmstadt, Yager, Hoffman, & Stayton, 2003;Xie et al, 2005).…”

mentioning

confidence: 99%

“…However, manufacturing conventional microfluidic devices of this type is expensive and virtually impossible without proper access to microfabrication facilities or machines. (Grinias & Kennedy, 2016). Furthermore, there are opportunities for multiplexing microfluidic chromatography systems with other types of microscale technologies but this effort will require more development before it is practical to implement.…”

mentioning

confidence: 99%

“…The discussion given above concerning process development tools for chromatography indicates that there is a need for optimization and process development ability at the microscale to help reduce cost of reagents and speed up biotherapeutic manufacturing for translation into the clinic (Pinto et al, 2017). Microfluidic devices have offered a platform that could potentially serve this need where less material is utilized to facilitate exploring novel approaches (Grinias & Kennedy, 2016;Kutter, 2012). The inherent small scale for these types of devices facilitates the development of portable, disposable, and modular chromatographic systems where various chromatographic processes are integrated onto a single device (Gaspar, Piyasena, & Gomez, 2007).…”

mentioning

confidence: 99%

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Low‐cost customizable microscale toolkit for rapid screening and purification of therapeutic proteins

Andar

Deldari

Gutierrez

et al. 2018

Biotech & Bioengineering

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Biopharmaceutical separations require tremendous amounts of optimization to achieve acceptable product purity. Typically, large volumes of reagents and biological materials are needed for testing different parameters, thus adding to the expense of biopharmaceutical process development. This study demonstrates a versatile and customizable microscale column (µCol) for biopharmaceutical separations using immobilized metal affinity chromatography (IMAC) as an example application to identify key parameters. µCols have excellent precision, efficiency, and reproducibility, can accommodate any affinity, ion-exchange or size-exclusion-based resin and are compatible with any high-performance liquid chromatography (HPLC) system. µCols reduce reagent amounts, provide comparable purification performance and high-throughput, and are easy to automate compared with current conventional resin columns. We provide a detailed description of the fabrication methods, resin packing methods, and µCol validation experiments using a conventional HPLC system. Finite element modeling using COMSOL Multiphysics was used to validate the experimental performance of the µCols. In this study, µCols were used for improving the purification achieved for granulocyte colony stimulating factor (G-CSF) expressed using a cell-free CHO in vitro translation (IVT) system and were compared to a conventional 1 ml IMAC column. Experimental data revealed comparable purity with a 10-fold reduction in the amount of buffer, resin, and purification time for the μCols compared with conventional columns for similar protein yields. K E Y W O R D Schromatography, granulocyte colony stimulating factor, immobilized metal affinity chromatography, microfluidics, protein purification

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Section: Design Considerationsmentioning

confidence: 99%

Section: Design Considerationsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Low‐cost customizable microscale toolkit for rapid screening and purification of therapeutic proteins

Andar

Deldari

Gutierrez

et al. 2018

Biotech & Bioengineering

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Microfluidic Systems in Analytical Chemistry

Gómez‐Hens

Fernández-Romero

2017

Encyclopedia of Analytical Chemistry

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This article provides an overview of the evolution of the different strategies described to improve the performance of microfluidic systems applied in Analytical Chemistry. The concept of analytical microfluidics here encompasses different approaches designed to implement these miniaturized systems for analytical purposes. The physical phenomena involved in microfluidic systems and the microfabrication techniques and materials used in these systems are briefly described. The strategies and difficulties in integrating the different steps of the analytical process, including sample pretreatment, separation, and detection, on a single microfluidic device are discussed. This article includes the most recent trends in the development of microfluidic analytical systems, such as the availability of new biosensors, the analytical usefulness of paper‐based microfluidic systems, the use of nanotechnology, and the applicability of these miniaturized systems to biochemical, food, and environmental analysis.

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Microfluidic Analysis Techniques for Safety Assessment of Pharmaceutical Nano‐ and Microsystems

Sikanen

Kiiski

Ollikainen

2020

Characterization of Pharmaceutical Nano and Microsystems

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Microfluidics is increasingly applied to chemical and biological research, including drug discovery and development This chapter gives an overview of the commonly used microfabrication methods and materials as well as microfluidic (bio)analytical techniques available for the in vitro safety assessment of pharmaceutical nano-and microsystems. A general overview will be given to the evolution of microfabrication methods and materials, which have facilitated the progressive development of microfluidic cell culture platforms (so called organ-on-a-chips), immobilized enzyme microreactors, and integrated separation systems (for chemical analysis). Of these techniques, the organ-on-a-chips are so far the most applied microfluidic concept in safety evaluation of pharmaceutical nano-and microsystems, whereas the other two represent techniques that could benefit the field in the future by enabling in-depth mechanism-based studies with respect to, e.g., improved hepatic safety.

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Advances in and prospects of microchip liquid chromatography

Cited by 71 publications

References 65 publications

Low‐cost customizable microscale toolkit for rapid screening and purification of therapeutic proteins

Low‐cost customizable microscale toolkit for rapid screening and purification of therapeutic proteins

Microfluidic Systems in Analytical Chemistry

Microfluidic Analysis Techniques for Safety Assessment of Pharmaceutical Nano‐ and Microsystems

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