Advances in analytical methodology for bioinorganic speciation analysis: metallomics, metalloproteomics and heteroatom-tagged proteomics and metabolomics

Szpunar, Joanna

doi:10.1039/b418265k

Cited by 373 publications

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References 276 publications

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“…6 The coupling of chromatography by using different separation mechanisms with inductively coupled plasma mass spectrometry (ICP-MS) detection has been the most widely used analytical tool for element speciation. [5][6][7][8] In the latest years, fruits and nuts from Amazon region have arisen interest among the scientific community due to the high concentrations of some essential elements in their compositions. [9][10][11][12][13][14][15][16][17] Special attention are devoted to Brazil-nut (Bertholletia excelsa), known as "castanha-doPará".…”

Section: Introductionmentioning

confidence: 99%

“…The protein standard was prepared dissolving 4.0 mg of ovoalbumin in 2.0 mL of deionized water, submitted to Vortex stirring for 2 min and diluted 10 times with deionized water. The spectrophotometer calibration was performed using analytical reference solutions of 4,6,8,10,12,16 and 20 μg of ovoalbumin in 1.0 mL of Bradford reagent.Water, NaCl, and NaOH extracts of the Brazil-nut samples were diluted 100 times and ethanol extract was diluted 10 times. A volume of 50 μL of these solutions was used for protein determination.…”

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confidence: 99%

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Cu, Fe, Mn and Zn distribution in protein fractions of Brazil-nut, cupuassu seed and coconut pulp by solid-liquid extraction and electrothermal atomic absorption spectrometry

Naozuka¹,

Oliveira²

2007

J. Braz. Chem. Soc.

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Neste trabalho foram determinados Cu, Fe, Mn and Zn em extratos de castanha-do-Pará (Bertholletia excelsa), semente de cupuaçu (Theobroma grandiflorum) e polpa de coco (Cocos nucifera) usando extração sólido-líquido e espectrometria de absorção atômica com atomização eletrotérmica. Foram empregadas diferentes soluções extratoras (mistura de metanol e clorofórmio 1:2 v/v, água, 0,5 mol L -1 NaCl, 70% v/v etanol e 0,5 mol L -1 NaOH) para extração de lipídios, albuminas, globulinas, prolaminas e glutelinas, respectivamente. As concentrações de proteínas encontradas na castanha-do-pará foram maiores do que aquelas encontradas na semente de cupuaçu e polpa de coco. Nas frações lipídicas foram encontradas maiores concentrações de Fe em castanhado-pará e semente de cupuaçu e Zn em polpa de coco. As determinações dos elementos nos extratos proteicos de castanha-do-pará indicaram que eles estão ligados, preferencialmente, às albuminas, globulinas e glutelinas. O mesmo comportamento não foi observado para semente de cupuaçu e polpa de coco. As concetrações dos elementos variaram para os diferentes extratos proteícos. Em geral, as maiores concentrações de proteínas foram determinadas nos extratos em NaOH. Por outro lado, as menores concentrações de Cu, Fe, Mn e Zn foram encontradas nessa fração.In this work Cu, Fe, Mn and Zn were determined in extracts of Brazil-nut (Bertholletia excelsa), cupuassu (Theobroma grandiflorum) seeds and coconut (Cocos nucifera) pulp, using solid-liquid extraction and electrothermal atomic absorption spectrometry. Different extractant solutions (mixture of methanol and chloroform 1:2 v/v, water, 0.5 mol L -1 NaCl, 70% v/v ethanol, and 0.5 mol L -1 NaOH) were used for extracting lipids, albumins, globulins, prolamins and glutelins, respectively. The protein concentrations determined in Brazil-nut are higher than those observed for cupuassu and coconut pulp. The analysis of lipid fractions reveals high concentations of Fe in Brazil-nut and cupuassu seed and Zn in coconut pulp. For Brazil-nut, these elements are mainly associated to albumin, globulin and glutelin fractions. The same behaviour was not observed for cupuassu seed and coconut pulp. The element concentrations changed in each protein fraction. In general, high concentrations of proteins were determined in NaOH extractants. On the other hand, low concentration of Cu, Fe, Mn and Zn were found in this fraction.Keywords: solid-liquid extraction, electrothermal atomic absorption spectrometry, Brazilnut, cupuassu, coconut IntroductionFoods are one of the main sources of metals for human body. Most of the metal ions present in foods are bound to specific proteins or enzymes and exert their effects as active or structural centers, command process such as catalysts, substrate binding and activation, transportation and storage. 1,2 In this way, the knowledge of the foods compositional, nutritional and functional properties is fundamental for defining their quality. In general, nutritional properties are characterized by both the abundance and bio...

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Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

Cu, Fe, Mn and Zn distribution in protein fractions of Brazil-nut, cupuassu seed and coconut pulp by solid-liquid extraction and electrothermal atomic absorption spectrometry

Naozuka¹,

Oliveira²

2007

J. Braz. Chem. Soc.

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“…Mineral compounds are formed and accumulated in the tissues as calcium oxalate, selenium, and nitrates; organic compounds are present in the form of: alkaloids, glycosides, toxalbumins, as well as extremely complex molecules that have not yet been identified (Board et al 2000, Nájera 1993, Szpunar 2005.…”

Section: Ethnobotany Research and Applications 78mentioning

confidence: 99%

“…These changes took place in association with new habits of life, especially in highly industrialized western societies, where natural drugs are considPublished: April 07, 2012 plants under certain conditions, and can be of either inorganic or organic nature. Mineral compounds are formed and accumulated in the tissues as calcium oxalate, selenium, and nitrates; organic compounds are present in the form of: alkaloids, glycosides, toxalbumins, as well as extremely complex molecules that have not yet been identified (Board et al 2000, Nájera 1993, Szpunar 2005). …”

Section: Introductionmentioning

confidence: 99%

Consequences of the Loss of Traditional Knowledge: The risk of injurious and toxic plants growing in kindergartens

Cuadra

Cambi

Rueda

et al. 2012

Ethnobot. Res. App.

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Education AbstractThe plant kingdom is a producer of poisons from a variety of toxic species. Nevertheless prevention of plant poisonings in Argentina is disregarded. As children are more affected, an evaluation of the dangerous plants present in kindergartens, and about the knowledge of teachers in charge about them, has been conducted. Floristic inventories and semi-structured interviews with teachers were carried out at 85 institutions of Bahía Blanca City. A total of 303 species were identified, from which 208 are considered to be harmless, 66 moderately and 29 highly harmful. Of the moderately harmful, 54% produce phytodematitis, and among the highly dangerous those with alkaloids and cyanogenic compounds predominate. The number of dangerous plants species present in each institution varies from none to 45. Kindergartens have no landscaping plan and the majority of teachers ignore the existence of toxic plants. Appropriate actions integrating education, prevention and valuation of the natural environment are needed. IntroductionAncient civilizations knew which plants were safe for human consumption and which were dangerous (Álvarez Arias 2000, Neuwinger 2004). This knowledge, transmitted orally from generation to generation, has almost been lost. As early as biblical times the fruits of some trees were associated with the concept of life, whereas others with death (Wagstaff 2008), underlining the close relationship between the history of toxic plants and traditional medicine (Al-Qura´n 2005). For a long time, nature was the only source of drugs, but, since the industrial revolution, people began to prefer synthetic products for pharmacological treatments. These changes took place in association with new habits of life, especially in highly industrialized western societies, where natural drugs are considPublished: April 07, 2012 Ethnobotany Research & Applications 78www.ethnobotanyjournal.org/vol10/i1547-3465-10-077.pdf plants under certain conditions, and can be of either inorganic or organic nature. Mineral compounds are formed and accumulated in the tissues as calcium oxalate, selenium, and nitrates; organic compounds are present in the form of: alkaloids, glycosides, toxalbumins, as well as extremely complex molecules that have not yet been identified (Board et al. 2000, Nájera 1993, Szpunar 2005.There are various hypotheses to explain the origin of toxic plant substances. They may be considered as waste products, intermediate products in metabolic processes or as secondary metabolites that fulfill non-essential functions in plants but intervene in plant/environment interactions. Some are pigments that give color to flowers and fruits, playing a fundamental role in reproduction by attracting insect pollinators or animals that contribute to seed dispersal. Others protect the plant from predators, acting as repellents due to their bitter taste and/or poisonous characteristics. They also intervene in the plants defense mechanisms against different pathogens, acting as natural pesticides. In some cases the e...

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“…This is another challenge being increasingly addressed in recent years. [54][55][56][57] In this sequence, two possibilities are described to perform online spike for ID quantification of species by ICP-MS. The basis for quantification of species by ID in flow systems involving different separation techniques coupled to ICP-MS is presented.…”

Section: Id Modes For Speciation Analysismentioning

confidence: 99%

Online isotope dilution and inductively coupled plasma mass spectrometry: from elemental to species quantification

Giné¹,

Packer²

2010

J. Braz. Chem. Soc.

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Apresentam-se sistemas de análises em fluxo propostos para a quantificação de elementos e espécies por diluição isotópica (ID) utilizando espectrometria de massas com fonte de plasma acoplada indutivamente (ICP-MS). Detalham-se os esquemas para adicionar em linha a solução isotopicamente enriquecida à amostra. São relatadas as aplicações em meio aquoso e outras com transferência do analito para a fase vapor, seja por processo de vaporização eletrotérmica, geração de vapor ou ablação por laser. Apresentam-se as aplicações com quantificação de espécies por ID caracterizadas pela adição do isótopo enriquecido, antes ou após a separação das espécies, seja por cromatografia a líquido, a gás ou por eletroforese capilar acoplada a ICP-MS. As principais equações envolvidas na quantificação por ID e as principais fontes de erro são descritas. São discutidas as mudanças introduzidas na estratégia de quantificação temporal por ID de elementos e espécies pela calibração do fluxo de massas da amostra e do isótopo enriquecido adicionado.Flow analysis has been utilized for elemental and species quantification by online isotope dilution inductively coupled plasma mass spectrometry (ICP-MS). Online isotope dilution (ID) herein refers to strategies promoting the addition of enriched isotopic solutions to the samples while flowing towards the plasma. Applications of online ID's are commonly performed in aqueous media, however, in some processes the analyte transference to the vapor phase using electrothermal vaporization, vapor generation or laser ablation is also required. Flow diagrams to perform online pre or post-column spiking with the enriched isotope for quantification by ID in speciation analysis by liquid chromatography, gas chromatography or capillary electrophoresis devices coupled to ICP-MS are presented. The main figures of merit and equations applied to elemental and species quantification by ID in flowing schemes are assessed.

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Advances in analytical methodology for bioinorganic speciation analysis: metallomics, metalloproteomics and heteroatom-tagged proteomics and metabolomics

Cited by 373 publications

References 276 publications

Cu, Fe, Mn and Zn distribution in protein fractions of Brazil-nut, cupuassu seed and coconut pulp by solid-liquid extraction and electrothermal atomic absorption spectrometry

Cu, Fe, Mn and Zn distribution in protein fractions of Brazil-nut, cupuassu seed and coconut pulp by solid-liquid extraction and electrothermal atomic absorption spectrometry

Consequences of the Loss of Traditional Knowledge: The risk of injurious and toxic plants growing in kindergartens

Online isotope dilution and inductively coupled plasma mass spectrometry: from elemental to species quantification

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