2002
DOI: 10.1016/s1570-0232(02)00498-1
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Advances in analysis of glycosaminoglycans: its application for the assessment of physiological and pathological states of connective tissues

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Cited by 51 publications
(56 citation statements)
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“…Treatment of CS isomers with chondro/dermato lyases is essential to determine disaccharides composition. The obtained D-disaccharides have been previously measured by either HPLC or CE by UVdetection at 232 nm due to the absorbance of the unsaturated disaccharide released by chondroitinases [21][22][23][24][25]. However, since plasma GAGs are found in trace amounts, highly sensitive methods are required for their analysis and structural characterization.…”
Section: Introductionmentioning
confidence: 99%
“…Treatment of CS isomers with chondro/dermato lyases is essential to determine disaccharides composition. The obtained D-disaccharides have been previously measured by either HPLC or CE by UVdetection at 232 nm due to the absorbance of the unsaturated disaccharide released by chondroitinases [21][22][23][24][25]. However, since plasma GAGs are found in trace amounts, highly sensitive methods are required for their analysis and structural characterization.…”
Section: Introductionmentioning
confidence: 99%
“…GAGs are highly anionic polysaccharides present in the extracellular matrix (ECM) and the cells. The biological importance of their fine structures in normal and disease state has been well demonstrated (1). They seem to play several roles in tumor formation because of their capacity to bind and modulate the action of a variety of proteins including growth factors, cytokines, and proteases.…”
Section: Introductionmentioning
confidence: 99%
“…Together with heparin, heparin sulfate, dermatan sulfate, keratan sulfate, and chondroitin sulfate (CS), it forms the group of connective tissue polysaccharides known as glycosaminoglycans (GAGs). HA has a high molecular mass (up to 8610 6 Da corresponding to about 10 000 disaccharide units), is not sulfated, and is not covalently bound to proteins [1][2][3][4][5][6]. HA is mainly produced by fibroblasts and other specialized connective tissue cells and participates in various cell-to-cell interactions.…”
Section: Introductionmentioning
confidence: 99%
“…Monitoring serum HA is also used in other areas, such as in septic conditions, tumor growth (such as Wilm's tumor and malignant mesothelioma), and for the diagnosis of rare hereditary diseases (such as Werner's syndrome and cutaneous hyaluronanosis) [4]. Many different colorimetric, chromatographic, and electrophoretic methods have been developed for the analysis of HA and other GAGs, their metabolic products, and the degraded molecules thereof [1,2]. Serum HA is currently determined with assays that are based upon the specific affinity of certain proteins to HA and that feature a low lg/L sensitivity [17], most notably a radiometric method (HA-test, Pharmacia, Uppsala, Sweden) and a sandwich enzyme-binding assay (Chugai Pharmaceutical, Tokyo, Japan; HA-test, Corgenix, Westminster, CO, USA).…”
Section: Introductionmentioning
confidence: 99%