Advances and challenges in bioprinting of biological tissues and organs

Tavafoghi, Maryam; Khademhosseini, Ali; Ahadian, Samad

doi:10.1111/aor.14069

“…By bioprinting patient-specific hNECs, it may be possible to create personalized treatments that are more effective and have fewer side effects. Despite the potential benefits, there are still challenges, such as biochemical properties, bioactivity and mechanical properties that need to be addressed before using such a system in clinical practice [94].…”

Section: Discussionmentioning

confidence: 99%

High-Throughput Bioprinting of the Nasal Epithelium using Patient-derived Nasal Epithelial Cells

Derman

¹

,

Yeo

²

,

Castaneda

³

et al. 2023

Preprint

0

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Human nasal epithelial cells (hNECs) are an essential cell source for the reconstruction of the respiratory pseudostratified columnar epithelium composed of multiple cell types in the context of infection studies and disease modeling. Hitherto, manual seeding has been the dominant method for creating nasal epithelial tissue models. However, the manual approach is slow, low-throughput and has limitations in terms of achieving the intricate 3D structure of the natural nasal epithelium in a uniform manner. 3D Bioprinting has been utilized to reconstruct various epithelial tissue models, such as cutaneous, intestinal, alveolar, and bronchial epithelium, but there has been no attempt to use of 3D bioprinting technologies for reconstruction of the nasal epithelium. In this study, for the first time, we demonstrate the reconstruction of the nasal epithelium with the use of primary hNECs deposited on Transwell inserts via droplet-based bioprinting (DBB), which enabled high-throughput fabrication of the nasal epithelium in Transwell inserts of 24-well plates. DBB of nasal progenitor cells ranging from one-tenth to one-half of the cell seeding density employed during the conventional cell seeding approach enabled a high degree of differentiation with the presence of cilia and tight-junctions over a 4-week air-liquid interface culture. Single cell RNA sequencing of these cultures identified five major epithelial cells populations, including basal, suprabasal, goblet, club, and ciliated cells. These cultures recapitulated the pseudostratified columnar epithelial architecture present in the native nasal epithelium and were permissive to respiratory virus infection. These results denote the potential of 3D bioprinting for high-throughput fabrication of nasal epithelial tissue models not only for infection studies but also for other purposes such as disease modeling, immunological studies, and drug screening.

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“…By bioprinting patient-specific hNECs, it may be possible to create personalized treatments that are more effective and have fewer side effects. Despite the potential benefits, there are still challenges, such as biochemical properties, bioactivity and mechanical properties that need to be addressed before using such a system in clinical practice [ 104 ].…”

Section: Discussionmentioning

confidence: 99%

High-throughput bioprinting of the nasal epithelium using patient-derived nasal epithelial cells

Derman

¹

,

Yeo

²

,

Castaneda

³

et al. 2023

Biofabrication

7

0

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Human nasal epithelial cells (hNECs) are an essential cell source for the reconstruction of the respiratory pseudostratified columnar epithelium composed of multiple cell types in the context of infection studies and disease modeling. Hitherto, manual seeding has been the dominant method for creating nasal epithelial tissue models. However, the manual approach is slow, low-throughput and has limitations in terms of achieving the intricate 3D structure of the natural nasal epithelium in a uniform manner. 3D Bioprinting has been utilized to reconstruct various epithelial tissue models, such as cutaneous, intestinal, alveolar, and bronchial epithelium, but there has been no attempt to use of 3D bioprinting technologies for reconstruction of the nasal epithelium. In this study, for the first time, we demonstrate the reconstruction of the nasal epithelium with the use of primary hNECs deposited on Transwell inserts via droplet-based bioprinting (DBB), which enabled high-throughput fabrication of the nasal epithelium in Transwell inserts of 24-well plates. DBB of nasal progenitor cells ranging from one-tenth to one-half of the cell seeding density employed during the conventional cell seeding approach enabled a high degree of differentiation with the presence of cilia and tight-junctions over a 4-week air-liquid interface culture. Single cell RNA sequencing of these cultures identified five major epithelial cells populations, including basal, suprabasal, goblet, club, and ciliated cells. These cultures recapitulated the pseudostratified columnar epithelial architecture present in the native nasal epithelium and were permissive to respiratory virus infection. These results denote the potential of 3D bioprinting for high-throughput fabrication of nasal epithelial tissue models not only for infection studies but also for other purposes such as disease modeling, immunological studies, and drug screening.

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“…Recently, 3D bioprinting has evolved as a novel biofabrication technology for the fabrication of 3D cell-laden constructs, using different biomaterials and cell types arranged in spatially defined locations. [7][8][9][10][11][12] Researchers have employed different 3D bioprinting strategies, such as dual 3D bioprinting to create a vascularized bone structure. 13 However, it is still challenging to print a bone construct including a well-defined network of vascular tissues incorporated within the hard matrix of bone tissue.…”

mentioning

confidence: 99%

Coaxial 3D bioprinting of tri‐polymer scaffolds to improve the osteogenic and vasculogenic potential of cells in co‐culture models

Shahabipour

¹

,

Tavafoghi

²

,

Aninwene

³

et al. 2022

J Biomedical Materials Res

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The crosstalk between osteoblasts and endothelial cells is critical for bone vascularization and regeneration. Here, we used a coaxial 3D bioprinting method to directly print an osteon-like structure by depositing angiogenic and osteogenic bioinks from the core and shell regions of the coaxial nozzle, respectively. The bioinks were made up of gelatin, gelatin methacryloyl (GelMA), alginate, and hydroxyapatite (HAp) nanoparticles and were loaded with human umbilical vascular endothelial cells (HUVECs) and osteoblasts (MC3T3) in the core and shell regions, respectively. Conventional monoaxial 3D bioprinting was used as a control method, where the hydrogels, HAp nanoparticles, MC3T3 cells, and HUVECs were all mixed in one bioink and printed from the core nozzle. As a result, the bioprinted scaffolds were composed of cell-laden fibers with either a core-shell or homogenous structure, providing a noncontact (indirect) or contact (direct) co-culture of MC3T3 cells and HUVECs, respectively. Both structures supported the 3D culture of HUVECs and osteoblasts over a long period. The scaffolds also supported the expression of osteogenic and angiogenic factors. However, the gene expression was significantly higher for the coreshell structure than the homogeneous structure due to the well-defined distribution of osteoblasts and endothelial cells and the formation of vessel-like structures in the co-culture system. Our results indicated that the coaxial bioprinting technique, with the ability to create a non-contact co-culture of cells, can provide a more efficient bioprinting strategy for printing highly vascularized and bioactive bone structures.

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Advances and challenges in bioprinting of biological tissues and organs

Cited by 3 publications

References 31 publications

High-Throughput Bioprinting of the Nasal Epithelium using Patient-derived Nasal Epithelial Cells

High-Throughput Bioprinting of the Nasal Epithelium using Patient-derived Nasal Epithelial Cells

High-throughput bioprinting of the nasal epithelium using patient-derived nasal epithelial cells

Coaxial 3D bioprinting of tri‐polymer scaffolds to improve the osteogenic and vasculogenic potential of cells in co‐culture models

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