Advancements in our understanding of circular and long non-coding RNAs in spinal cord injury

Han, Wei; Ding, Yuchuan; Zhang, Yan; Yun, HoJun; Ji, Yu; Cosky, Eric E; Zhang, Wenxiu

doi:10.4103/1673-5374.335835

Cited by 6 publications

(4 citation statements)

References 52 publications

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“…[28] Previous studies have shown that lncRNAs are associated with the progression of SCI. [10,29] According to Zhang et al, lncRNA DGCR5 suppresses neuronal cell apoptosis to ameliorate acute spinal cord damage by targeting PRDM5. [30] Li et al reported that lncRNA ZNF667-AS1 can suppress the janus kinase-signal transducer and activator of transcription pathway, which leads to the inhibition of the inflammatory response and enhances SCI recovery.…”

Section: Discussionmentioning

confidence: 99%

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LncRNA TSIX aggravates spinal cord injury by regulating the PI3K/AKT pathway via the miR‐532‐3p/DDOST axis

Dong

Wei

Zhu

2023

J Biochem & Molecular Tox

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Long noncoding RNA (lncRNA)‐X‐inactive‐specific transcript (TSIX) expression is upregulated in spinal cord tissues following spinal cord injury (SCI). However, the role of lncRNA‐TSIX in SCI remains elusive. SCI animal model was established using C57BL/6 mice. LncRNA TSIX and miR‐532‐3p expression were determined using quantitative reverse transcription polymerase chain reaction (qRT‒PCR). Apoptosis, cell proliferation, and migration were evaluated by transferase dUTP nick end labeling staining, CCK‐8, and Transwell assays, respectively. The interaction of miR‐532‐3p with lncRNA TSIX and DDOST was explored via a dual‐luciferase reporter system. Hematoxylin‐eosin staining and the Basso, Beattie, and Bresnahan locomotor rating (BBB) scale were performed to investigate SCI progression. The expression of the lncRNA TSIX was found to be significantly upregulated in the serum of SCI patients and spinal cord tissues of SCI mice. The overexpression of lncRNA TSIX enhanced spinal cord neural stem cell (SC‐NSC) proliferation and migration in vitro while inhibiting apoptosis and inflammatory cell infiltration in vivo. Moreover, lncRNA TSIX acted as a molecular sponge for miR‐532‐3p, and the knockdown of miR‐532‐3p promoted proliferation and migration and inhibited apoptosis of SC‐NSCs. Moreover, DDOST was found to be the downstream target of miR‐532‐3p, and DDOST overexpression showed a similar effect as miR‐532‐3p silencing on the proliferation, migration, and apoptosis of SC‐NSCs. Furthermore, we found that lncRNA TSIX overexpression promoted the activation of the PI3K/AKT signaling pathway. LncRNA TSIX aggravates SCI by regulating the PI3K/AKT pathway via the miR‐532‐3p/DDOST axis, indicating potential applications for targeted therapy of SCI regeneration.

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Section: Discussionmentioning

confidence: 99%

“…[8,9] Researchers have revealed the critical roles of lncRNAs in SCI. [10,11] X-inactive-specific transcript (XIST) refers to a class of noncoding RNAs implicated in the differentiation and proliferation of cells. [12] Previous studies have revealed that XIST promotes neurological repair post-SCI by binding to miR-32-5p to modulate Notch-1.…”

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confidence: 99%

LncRNA TSIX aggravates spinal cord injury by regulating the PI3K/AKT pathway via the miR‐532‐3p/DDOST axis

Dong

Wei

Zhu

2023

J Biochem & Molecular Tox

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“…A spinal cord injury (SCI) is one of the most common central nervous system disorders (J. ; Zhang et al 2022). SCI maybe devastating, due to the CNS unable to effectively regenerate (Nazareth et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Protective effect of bone morphogenetic protein-7 induced differentiation of bone marrow mesenchymal stem cells in rat with acute spinal cord injury

Sun

Huang

et al. 2023

Funct Integr Genomics

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The principal aim of present study was to assess the therapeutic e cacy of bone morphogenetic protein-7 (BMP-7) induced bone marrow mesenchymal stem cells (BMSCs) differentiation in rat acute spinal cord injury (SCI) model. BMSCs were isolated from rats, then divided into control and BMP7 induction groups.The proliferation ability of BMSCs and glial cell markers were detected. Forty Sprague-Dawley (SD) rats were randomly divided into sham, SCI, BMSCs, and BMP7 + BMSCs groups (n = 10). The recovery of motor function of hind limbs of rats, the pathological, related markers and motor evoked potentials (MEP) were detected. BMSCs differentiated into neuron like cells after exogenous BMP7 intervention. Interestingly, the expression levels of MAP-2 and Nestin increased, while the expression GFAP level decreased after exogenous BMP7 intervention. BBB score reached 19.33 ± 0.58 in the BMP7 + BMSCs group at day 42. Nissl bodies in model group were reduced compared to sham group. After 42 days, both in the BMSCs and BMP7 + BMSCs groups, the number of nissl bodies were increased. Especially, the number of nissl bodies in the BMP7 + BMSCs group was more than that in BMSCs group. The expression of Tuj-1 and MBP in BMP7 + BMSCs group increased, while the expression of GFAP decreased. MEP waveform decreased signi cantly after surgery. The waveform was wider, the amplitude was higher in BMP7 + BMSCs group than that in BMSCs group. BMP7 promotes BMSCs proliferation, and induces BMSCs differentiate into neuron like cells, inhibits the formation of glial scar. BMP7 plays a con dent role in the recovery of SCI rats.

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“…It typically consists of the rupture of blood vessels and cell membranes. The second stage is the pathophysiological response and typically involves ischemia, apoptosis, and hypoxia ( Zhang et al, 2022 ). SCI results in massive social, physical, and lifelong healthcare costs to the community and society ( Chen et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Granulocyte colony-stimulating factor effects on neurological and motor function in animals with spinal cord injury: a systematic review and meta-analysis

et al. 2023

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BackgroundSpinal cord injury (SCI) is a severe neurological injury for which no effective treatment exists. Granulocyte colony-stimulating factor (G-CSF) is used to treat autologous bone marrow transplantation, chemotherapy-induced granulocytopenia, Acquired Immune Deficiency Syndrome (AIDS), etc. Recent research has revealed the potential application of G-CSF on neuroprotective effectiveness. In central nervous system diseases, G-CSF can be used to alleviate neuronal injury.ObjectiveTo investigate the effects of G-CSF on Basso, Beattie, and Bresnahan (BBB) scale score, inclined plane test, electrophysiologic exam, quantitative analysis of TUNEL-positive cells, and quantitative analysis of glial fibrillary acidic protein (GFAP) immunostaining images in animal models of SCI.MethodsWe searched PubMed, Web of Science, and Embase databases for all articles on G-CSF intervention with animal models of SCI reported before November 2022. A total of 20 studies met the inclusion criteria.ResultsResults revealed that G-CSF intervention could improve the BBB scale score in both groups at 3, 7, 14, 28, and 35 days [at 35 days, weighted mean differences (WMD) = 2.4, 95% CI: 1.92–2.87, p < 0.00001, I2 = 69%]; inclined plane test score; electrophysiologic exam; quantitative analysis of TUNEL-positive cell numbers; quantitative analysis of GFAP immunostaining images in animal models of SCI. Subgroup analysis revealed that treatment with normal saline, phosphate-buffered saline, and no treatment resulted in significantly different neurological function effectiveness compared to the G-CSF therapy. SD rats and Wistar rats with SCI resulted in significant neurological function effectiveness. C57BL/6 mice showed no difference in the final effect. The T9–T10 or T10 segment injury model and the T8–T9 or T9 segment injury model resulted in significant neurological function effectiveness. The BBB score data showed no clear funnel plot asymmetry. We found no bias in the analysis result (Egger’s test, p = 0.42). In our network meta-analysis, the SUCRA ranking showed that 15 mg/kg-20 mg/kg was an optimal dose for long-term efficacy.ConclusionOur meta-analysis suggests that G-CSF therapy may enhance the recovery of motor activity and have a specific neuroprotective effect in SCI animal models.Systematic review registration: PROSPERO, identifier: CRD42023388315.

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Advancements in our understanding of circular and long non-coding RNAs in spinal cord injury

Cited by 6 publications

References 52 publications

LncRNA TSIX aggravates spinal cord injury by regulating the PI3K/AKT pathway via the miR‐532‐3p/DDOST axis

LncRNA TSIX aggravates spinal cord injury by regulating the PI3K/AKT pathway via the miR‐532‐3p/DDOST axis

Protective effect of bone morphogenetic protein-7 induced differentiation of bone marrow mesenchymal stem cells in rat with acute spinal cord injury

Granulocyte colony-stimulating factor effects on neurological and motor function in animals with spinal cord injury: a systematic review and meta-analysis

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