Protective effect of bone morphogenetic protein-7 induced differentiation of bone marrow mesenchymal stem cells in rat with acute spinal cord injury

Abstract

The principal aim of present study was to assess the therapeutic e cacy of bone morphogenetic protein-7 (BMP-7) induced bone marrow mesenchymal stem cells (BMSCs) differentiation in rat acute spinal cord injury (SCI) model. BMSCs were isolated from rats, then divided into control and BMP7 induction groups.The proliferation ability of BMSCs and glial cell markers were detected. Forty Sprague-Dawley (SD) rats were randomly divided into sham, SCI, BMSCs, and BMP7 + BMSCs groups (n = 10). The recovery of motor function of hind limbs of rats, the pathological, related markers and motor evoked potentials (MEP) were detected. BMSCs differentiated into neuron like cells after exogenous BMP7 intervention. Interestingly, the expression levels of MAP-2 and Nestin increased, while the expression GFAP level decreased after exogenous BMP7 intervention. BBB score reached 19.33 ± 0.58 in the BMP7 + BMSCs group at day 42. Nissl bodies in model group were reduced compared to sham group. After 42 days, both in the BMSCs and BMP7 + BMSCs groups, the number of nissl bodies were increased. Especially, the number of nissl bodies in the BMP7 + BMSCs group was more than that in BMSCs group. The expression of Tuj-1 and MBP in BMP7 + BMSCs group increased, while the expression of GFAP decreased. MEP waveform decreased signi cantly after surgery. The waveform was wider, the amplitude was higher in BMP7 + BMSCs group than that in BMSCs group. BMP7 promotes BMSCs proliferation, and induces BMSCs differentiate into neuron like cells, inhibits the formation of glial scar. BMP7 plays a con dent role in the recovery of SCI rats.

Myelin repair of spinal cord injury in adult mice induced by treadmill training upregulated peroxisome proliferator‐activated receptor gamma coactivator 1 alpha

“…The stimulation electrode lightly touched the pia meninge, while the recording electrodes were inserted into the left gastrocnemius muscle, with a 0.5 cm interval between the positive and negative polarities. The reference electrode was clamped near the scalp incision, while the ground electrode was linked to the tail (Sun et al, 2023; Xu et al, 2023). The stimulation frequency and the time constant were set at 4 Hz and 0.2 ms, respectively.…”

Potential of bone morphogenetic protein-7 in treatment of lupus nephritis: addressing the hurdles to implementation

“…For example, glomerular podocyte apoptosis, which is linked to glomerular sclerosis and which is induced by TGF-β activation of the p38 MAPK pro-apoptotic pathway, was opposed by BMP-7 activation of the pro-survival pathway PI3K/Akt (Peters et al 2006 ). Similarly, BMP-7 is known to upregulate expression of nestin—a cytoskeletal protein known to protect podocytes from injury in LN (Tian et al 2020 )—to promote regenerative outcome (Zhang et al 2013 ; Sun et al 2023 ).…”