Advanced Properties of Urine Derived Stem Cells Compared to Adipose Tissue Derived Stem Cells in Terms of Cell Proliferation, Immune Modulation and Multi Differentiation

Abstract

Adipose tissue stem cells (ADSCs) would be an attractive autologous cell source. However, ADSCs require invasive procedures, and has potential complications. Recently, urine stem cells (USCs) have been proposed as an alternative stem cell source. In this study, we compared USCs and ADSCs collected from the same patients on stem cell characteristics and capacity to differentiate into various cell lineages to provide a useful guideline for selecting the appropriate type of cell source for use in clinical application. The urine samples were collected via urethral catheterization, and adipose tissue was obtained from subcutaneous fat tissue during elective laparoscopic kidney surgery from the same patient (n = 10). Both cells were plated for primary culture. Cell proliferation, colony formation, cell surface markers, immune modulation, chromosome stability and multi-lineage differentiation were analyzed for each USCs and ADSCs at cell passage 3, 5, and 7. USCs showed high cell proliferation rate, enhanced colony forming ability, strong positive for stem cell markers expression, high efficiency for inhibition of immune cell activation compared to ADSCs at cell passage 3, 5, and 7. In chromosome stability analysis, both cells showed normal karyotype through all passages. In analysis of multi-lineage capability, USCs showed higher myogenic, neurogenic, and endogenic differentiation rate, and lower osteogenic, adipogenic, and chondrogenic differentiation rate compared to ADSCs. Therefore, we expect that USC can be an alternative autologous stem cell source for muscle, neuron and endothelial tissue reconstruction instead of ADSCs.

Isolation and in vitro cultivation of human urine-derived cells: an alternative stem cell source

“…Additionally, recent studies have indicated that the cells isolated from voided urine or urine catheterized from urinary tract have multi-lineage differentiation capability which are highly expandable and posses stem cell features. [1,2,[10][11][12][13] One of the most important results of the present study is that that human urine contains mesenchymal stem cells together with urothelial cells. This result is in compliance with the results other studies published in recent years.…”

Human Urine-Derived Stem Cell Differentiation to Endothelial Cells with Barrier Function and Nitric Oxide Production

“…These cells, originating from the parietal cells in kidney glomeruli , can be obtained using noninvasive, simple, safe, and low‐cost procedures, avoiding adverse events associated with current therapies. USCs can be easily isolated and expanded , which offers clear advantages over stem cells from other sources . After optimizing our methods, 100–140 USC clones/24 hours urine collection were consistently obtained from each individual .…”

Retracted: Exosomes secreted by human urine‐derived stem cells accelerate skin wound healing by promoting angiogenesis in rat by Yuan H, Guan J, Zhang J, Zhang R, Li M

“…They express strong positive markers as stem cells, but have a unique inhibition on immune cell activation compared to adipose derived stem cells (ADSCs); However, hUSCs showed lower osteogenic, adipogenic, and chondrogenic differentiation rate compared to ADSCs (Kang et al, 2015). Studies have demonstrated that hUSCs expressed urothelial markers after they were seeded onto a bacterial cellulose polymer (Bodin et al, 2010).…”