Advanced platelet rich fibrin demonstrates improved osteogenic induction potential in human periodontal ligament cells, growth factor production and mechanical properties as compared to leukocyte and platelet fibrin and injectable platelet rich fibrin

Shah, Rucha; M. G., Triveni; Thomas, Raison; A. B., Tarun Kumar

doi:10.1007/s10006-023-01160-8

Cited by 3 publications

(2 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, Shah et al detected solid PRF superior to injectable PRF in terms of mechanical strength and increased growth factor releases of TGF-b, PDGF-BB, and VEGF. This enhanced cell viability, alkaline phosphatase production, and mineralization on human periodontal ligament cells [42]. Concerning the presented results, there was no signi cant difference in vascularisation when the membrane was biofunctionalized using solid or injectable PRF.…”

Section: Discussionmentioning

confidence: 55%

Platelet Rich Fibrin for rehydration and pre-vascularization of an acellular, collagen membrane of porcine origin

Schröger,

Blatt,

Sagheb

et al. 2023

Preprint

View full text Add to dashboard Cite

Objectives Pre-Vascularization of collagen membranes with autologous platelet concentrates is a standard procedure in oral and maxillofacial surgery. This study analyzed the possible interaction of an acellular collagen membrane of porcine origin (NM) with platelet-rich fibrin (PRF) regarding its rehydration protocol with differences in pH values and effect on angiogenesis. Materials and methods NM was analyzed alone and combined with solid PRF by plotting or co-culturing with injectable PRF. Different media (venous blood, buffer solution with a fixed pH value of 7, saline solution, and injectable PRF) were used to analyze the influence on pH value during rehydration. Chorion allantois membrane assay (CAM) was applied to check pro-angiogenic effects after 24, 48, and 72 hours, followed by immunohistochemical analysis. Results Rehydration in injectable PRF showed acidity over time (p < 0.05). A definite pro-angiogenic effect of NM alone was found regarding neo-vessel formation supported by the respective light microscopically analysis without significant differences to PRF alone (p>0.005). This pro-angiogenic effect could not be exaggerated when NM was combined with liquid/solid PRF (each p>0.005). Conclusions Rehydration with liquid PRF of the collagen membrane results in acidity compared to a saline solution or patient’s blood. The significant pro-angiogenic potential of the membrane alone resulted in enhanced neo-vessel formation that could not be optimized with the addition of PRF.

show abstract

Section: Discussionmentioning

confidence: 55%

Platelet Rich Fibrin for rehydration and pre-vascularization of an acellular, collagen membrane of porcine origin

Schröger,

Blatt,

Sagheb

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…In contrast, Shah et al detected solid PRF superior to injectable PRF in terms of mechanical strength and increased growth factor releases of TGF-b, PDGF-BB, and VEGF. This enhanced cell viability, alkaline phosphatase production, and mineralization in human periodontal ligament cells [ 43 ]. Concerning the presented results, there was no significant difference in vascularisation when the membrane was biofunctionalized using solid or injectable PRF.…”

Section: Discussionmentioning

confidence: 99%

Platelet-rich fibrin for rehydration and pre-vascularization of an acellular, collagen membrane of porcine origin

Schröger,

Blatt,

Sagheb

et al. 2024

Clin Oral Invest

View full text Add to dashboard Cite

Objectives Pre-vascularization of the collagen membranes with autologous platelet concentrates is a standard procedure in oral and maxillofacial surgery. This study analyzed the possible interaction of an acellular collagen membrane of porcine origin (NM) with platelet-rich fibrin (PRF) regarding its rehydration protocol with differences in pH values and effect on angiogenesis. Materials and methods NM was analyzed alone and combined with solid PRF by plotting or co-culturing with injectable PRF. Different media (venous blood, buffer solution with a fixed pH value of 7, saline solution, and injectable PRF) were used to analyze the influence on pH value during rehydration. Chorion allantois membrane assay (CAM) was applied to check pro-angiogenic effects after 24, 48, and 72 h, followed by immunohistochemical analysis. Results Rehydration in injectable PRF showed acidity over time (p < 0.05). A definite pro-angiogenic effect of NM alone was found regarding neo-vessel formation supported by the respective light microscopically analysis without significant differences to PRF alone (p > 0.005). This pro-angiogenic effect could not be exaggerated when NM was combined with liquid/solid PRF (each p > 0.005). Conclusions Rehydration with liquid PRF of the collagen membrane results in acidity compared to a saline solution or patient’s blood. The significant pro-angiogenic potential of the membrane alone resulted in enhanced neo-vessel formation that could not be optimized with the addition of PRF. Clinical relevance statement Using injectable PRF for rehydration protocol of the collagen membrane leads to acidosis that can ultimately optimize wound healing. Differences in the physio-mechanical interplay of collagen matrices and autologous platelet concentrates must result in clinical algorithms if pre-vascularization can maximize outcomes.

show abstract

Effectiveness of Advanced Platelet-Rich Fibrin on Postoperative Sequelae for Impacted Mandibular Third Molar Surgery: A Prospective Study

Mathialagan Kalai Selvam,

Lakshmanan

et al. 2024

Cureus

View full text Add to dashboard Cite

Introduction Impacted third molar extraction is frequently removed surgically by maxillofacial surgeons, which is mostly associated with postoperative sequelae like pain and swelling. It is essential to minimize the complications and enhance wound healing in the extracted socket of the third molar. Hence, this study aimed to assess the efficiency of advanced platelet-rich fibrin (A-PRF) in wound healing and reducing pain after surgical extraction of the impacted mandibular third molar (IMTM). Materials and methods Thirty healthy patients who have been diagnosed with Pell and Gregory class II IMTM were included in this study. In the study group, which comprises 15 patients, extraction sockets were filled with A-PRF extract. In the control group, no material was placed in the extraction sockets. The pain was assessed preoperative and on the third and seventh postoperative days using a visual analog scale (VAS). Wound healing was assessed on the third and seventh postoperative days using a modified laundry scale. SPSS for Windows was used for data analysis. Categorical data was compared between the groups using the Chi-square test. P-value less than 0.05 was considered as statistically significant. Results The study population's mean age was 25.67 ± 2.4 years. Nineteen patients were male, and 11 patients were female. Differences in mean pain scores between the groups were not statistically significant both on the third postoperative day (p=0.59) and the seventh postoperative day (p=0.33). During the seventh day postoperative day, the study group exhibited better wound healing compared to the control group and the results were statistically significant (p=0.01). Conclusion A-PRF is a simple and effective method of reducing postoperative sequela by promoting wound healing after surgical extraction of IMTM. It has the advantage of less chance of allergic and anaphylactic reactions, unlike their predecessor platelet concentrates.

show abstract

Advanced platelet rich fibrin demonstrates improved osteogenic induction potential in human periodontal ligament cells, growth factor production and mechanical properties as compared to leukocyte and platelet fibrin and injectable platelet rich fibrin

Cited by 3 publications

References 48 publications

Platelet Rich Fibrin for rehydration and pre-vascularization of an acellular, collagen membrane of porcine origin

Platelet Rich Fibrin for rehydration and pre-vascularization of an acellular, collagen membrane of porcine origin

Platelet-rich fibrin for rehydration and pre-vascularization of an acellular, collagen membrane of porcine origin

Effectiveness of Advanced Platelet-Rich Fibrin on Postoperative Sequelae for Impacted Mandibular Third Molar Surgery: A Prospective Study

Contact Info

Product

Resources

About