Advanced oxidation process sanitization of eggshell surfaces

Abstract

The microbial quality of eggs entering the hatchery represents an important critical control point for biosecurity and pathogen reduction programs in integrated poultry production. The development of safe and effective interventions to reduce microbial contamination on the surface of eggs will be important to improve the overall productivity and microbial food safety of poultry and poultry products. The hydrogen peroxide (H2O2) and ultraviolet (UV) light advanced oxidation process is a potentially important alternative to traditional sanitizers and disinfectants for egg sanitation. The H2O2/UV advanced oxidation process was demonstrated previously to be effective in reducing surface microbial contamination on eggs. In this study, we evaluated treatment conditions affecting the efficacy of H2O2/UV advanced oxidation in order to identify operational parameters for the practical application of this technology in egg sanitation. The effect of the number of application cycles, UV intensity, duration of UV exposure, and egg rotation on the recovery of total aerobic bacteria from the surface of eggs was evaluated. Of the conditions evaluated, we determined that reduction of total aerobic bacteria from naturally contaminated eggs was optimized when eggs were sanitized using 2 repeated application cycles with 5 s exposure to 14 mW cm(-2) UV light, and that rotation of the eggs between application cycles was unnecessary. Additionally, using these optimized conditions, the H2O2/UV process reduced Salmonella by greater than 5 log10 cfu egg(-1) on the surface of experimentally contaminated eggs. This study demonstrates the potential for practical application of the H2O2/UV advanced oxidation process in egg sanitation and its effectiveness in reducing Salmonella on eggshell surfaces.

Preliminary study: Health and performance assessment in broiler chicks following application of six different hatching egg disinfection protocols

“…The use of peracetic acid on hatching eggs lead to a significant reduction of aerobic bacteria and a significant reduction of Enterobacteriaceae and was suggested as possible alternative for formaldehyde hatching egg disinfection. [21] UV light was tested alone [22] and in combination with hydrogen peroxide [23] with a favorable effect on bacterial contamination due to advanced oxidation processes resulting in the production of highly reactive oxygen species. [23] A disadvantage was heat development when application times exceeded 8 minutes.…”

“…[21] UV light was tested alone [22] and in combination with hydrogen peroxide [23] with a favorable effect on bacterial contamination due to advanced oxidation processes resulting in the production of highly reactive oxygen species. [23] A disadvantage was heat development when application times exceeded 8 minutes. [12] Quaternary ammonium preparations appeared to decrease hatchability with increasing concentrations [20], but also did not successfully eliminate bacteria from the egg shell.…”

Eggshell microbiology and quality of hatching eggs subjected to different sanitizing procedures

“…For irradiation with type C ultraviolet light, using disposable latex gloves, the eggs were placed one by one on aluminum trays with a capacity of 95 eggs and then put inside a closed chamber, where 16 UV-C lamps -30 W, 90 cm, and 254 nm (model G30T8 UVC, Halo Tech Co., Ltd., Shenzhen, China) -provided an average light intensity of 6.36 mW cm -2 , as adapted from Gottselig et al (2016). The egg trays, designed to prevent eggs from touching each other and, consequently, to allow a greater exposure to UV-C light, were positioned at the center of the chamber to allow the irradiation from eight lamps positioned above the entire extension of the tray and from eight lamps at the bottom of the chamber.…”

Combined nanofiltration and advanced oxidation processes with bifunctional carbon nanomembranes

“…Advanced oxidation processes (AOP) characterized by the generation of highly reactive, non-selective hydroxyl radicals offer a promising alternative to conventional treatment for removing organic pollutants. [10][11][12][13] The ability of hydroxyl radicals to fully mineralize the micro-organic pollutants, or at least to produce less harmful intermediates, makes AOP an integral part of wastewater treatment. The most common process used to generate hydroxyl radicals is the combined use of catalytic oxidants such as ozoneultraviolet, hydrogen peroxide-ultraviolet, and hydrogen peroxideozone.…”