Advanced Multiplex PCR Assay for Differentiation of Brucella Species

Kang, Sung-Il; Her, Moon; Kim, Jong Wan; Kim, Jiyeon; Ko, Kyung Yuk; Ha, Yun-Mi; Jung, Suk-Chan

doi:10.1128/aem.00581-11

Cited by 50 publications

(43 citation statements)

References 17 publications

(20 reference statements)

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“…Vaccine strains B. abortus S19, B. melitensis Rev.1 and B. abortus RB51 may be identified using specific PCRs (Kang et al, 2011;Lopez-Goňi et al, 2011), or by their growth characteristics in culture.…”

Section: In Infected Herds/flocks a Positive Results To Any Serologicmentioning

confidence: 99%

Isolation of Brucella pinnipedialis from Grey Seals (Halichoerus grypus) in the Baltic Sea

Hirvelä-Koski

Nylund

Skrzypczak

et al. 2017

Journal of Wildlife Diseases

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Description of the disease: Brucellosis is the generic name used for the animal and human infections caused by several species of the genusCausal pathogens: Genetic and immunological evidence indicates that all members of the Brucella genus are closely related. Nevertheless, based on relevant differences in host preference and epidemiology displayed by the major variants, as well as molecular evidence of genomic variation, the International Committee on Systematics of Prokaryotes, Subcommittee on the Taxonomy of Brucella took a clear position in 2005 on a return to pre-1986 Brucella taxonomic opinion; the consequences of this statement imply the re-approval of the six classical Brucella nomenspecies with their corresponding recognised biovars. The classical names related to the six Brucella nomenspecies are validly published in the Approved Lists of Bacterial Names, 1980, and the designated type strains are attached to these validly published names: B. abortus, B. melitensis, B. suis, B. neotomae, B. ovis and B. canis 1 . The first three of these are subdivided into biovars based on cultural and serological properties (see Tables 2 and 3). Strains of Brucella have been isolated in the last decade from marine mammals but these strains cannot be ascribed to any of the above-recognised species. Investigations are continuing to establish their proper position in the taxonomy of the genus and it has been proposed that they be classified into two new species: B. ceti and B. pinnipedialis (Foster et al., 2007). A new species, named B. microti, was also isolated from the common vole (Microtus arvalis) as well as from foxes and soil in Central Europe (Scholz et al., 2008). Novel isolates from human breast implant and lung infections (strains BO1 and BO2) and from baboons that had delivered stillborn offspring have also been described, although the natural reservoir of these isolates remains unclear. While only two isolates of each new type have been described, they have been formally published as the tenth and eleventh Brucella species, B. inopinata and B. papionis respectively (Scholz et al., 2010;Whatmore et al., 2014). Finally, strains isolated from rodents, foxes and frogs were characterised as atypical Brucella strains distinct from the currently described species. They have not yet been approved as new Brucella species.Brucella is a member of the Brucellaceae family, in the order Rhizobiales, class Alphaproteobacteria. It shows close genetic relatedness to some plant pathogens and symbionts of the genera Agrobacterium and Rhizobium, as well as animal pathogens (Bartonella) and opportunistic or soil bacteria (Ochrobactrum).Infection with Brucella in cattle is usually caused by biovars (bv.) of Brucella abortus.

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Section: In Infected Herds/flocks a Positive Results To Any Serologicmentioning

confidence: 99%

Isolation of Brucella pinnipedialis from Grey Seals (Halichoerus grypus) in the Baltic Sea

Hirvelä-Koski

Nylund

Skrzypczak

et al. 2017

Journal of Wildlife Diseases

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“…With the aim of differentiating the species B.ceti and B. pinnipedialis, additional primers were used [34,38,39]. Correct species could still not be determined with certainty.…”

Section: Brucellosis In Marine Mammals In the Republic Of Croatiamentioning

confidence: 99%

Brucellosis in marine mammals, with special emphasis on the Republic of Croatia

Cvetnić¹,

Duvnjak²,

Đuras³

et al. 2017

Rad Hrvatske akademije znanosti i umjetnosti

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SummaryAfter almost two decades of research and evidence of brucellosis in marine mammals, it has become widely known that this disease is prevalent in marine mammals on a global scale. Positive animals have been found in the northern Atlantic, Mediterranean Sea and in the Arctic, including the Barents Sea. Infected or exposed animals have been detected along the Atlantic and Pacific coasts of North America, off the coasts of Peru, Australia, New Zealand, Hawaii, the Solomon Islands, and in the Antarctic Ocean. Brucellosis most commonly occurs in the harbour porpoise (Phocoena phocoena), followed by the striped dolphin (Stenella coeruleoalba), Atlantic white-sided dolphin (Lagenorhynchus acutus), bottlenose dolphin (Tursiops truncatus), shortbeaked common dolphin (Delphinus delphis) and minke whale (Balaenoptera acutorostrata). A positive serological reaction has been confirmed in 35 species of whales and 14 species of seals, two subspecies of sea otters, one species of freshwater otter and the polar bear. To date, brucellosis has been found in marine mammals in the Mediterranean Sea in Italy and Spain. The first research on brucellosis in dolphins in the Republic of Croatia began in 2015. Brucella sp. was isolated from the lymph node of a bottlenose dolphin found dead in the Poreč region. This was the first evidence of brucellosis in dolphins in the Adriatic Sea. The isolated sample was identified as Brucella ceti strain ST27, making this the first record of this strain in Europe, which may present a significant threat to human health.

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“…They are B. melitensis, Brucella abortus (B. abortus), Brucella suis (B. suis) and Brucella canis (B. canis). B. abortus is found principally in cattle, B. melitensis in goats and sheep, B. suis in swine and B. canis in kennelraised dogs [3] .…”

Section: Introductionmentioning

confidence: 99%