Clinical human brucellosis in Malaysia: a case report

Hartady, Tyagita; Saad, Mohd Zamri; Bejo, Siti Khairani; Salisi, Mohd Shahrom

doi:10.1016/s2222-1808(14)60332-7

Cited by 19 publications

(13 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In China, a case of subdural empyema complicated by intracerebral abscess due to Brucella infection was effectively treated with antibiotic therapy (ceftriaxone, doxycycline, rifapentine) [ 108 ]. In line with this, several reports suggested the combination therapy of doxycycline and rifampicin for six weeks is enough to eradicate Brucella infection, as well as associated complications [ 46 , 109 , 110 , 111 ]. This combination of doxycycline and rifampicin has also been proven experimentally [ 112 ].…”

Section: Treatment Of Brucellosismentioning

confidence: 81%

“…Brucella infection is widespread in several South Asian/Asian countries including Pakistan, India, China, and Sri Lanka, in humans as well as in animals [ 42 , 43 , 44 , 45 ]. In 1950, Brucella was for the first time reported in animals in Malaysia, and the government undertook an eradication strategy for bovine, ovine, and caprine brucellosis (National Surveillance Program for Animal Brucellosis) since 1978 [ 46 ]. Additionally, a series of studies documented the seropositive cases of brucellosis in humans mainly in veterinary professionals and farmers that had close contact with animals.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

An Overview of Brucellosis in Cattle and Humans, and its Serological and Molecular Diagnosis in Control Strategies

2018

View full text Add to dashboard Cite

Brucellosis is one of the most common contagious and communicable zoonotic diseases with high rates of morbidity and lifetime sterility. There has been a momentous increase over the recent years in intra/interspecific infection rates, due to poor management and limited resources, especially in developing countries. Abortion in the last trimester is a predominant sign, followed by reduced milk yield and high temperature in cattle, while in humans it is characterized by undulant fever, general malaise, and arthritis. While the clinical picture of brucellosis in humans and cattle is not clear and often misleading with the classical serological diagnosis, efforts have been made to overcome the limitations of current serological assays through the development of PCR-based diagnosis. Due to its complex nature, brucellosis remains a serious threat to public health and livestock in developing countries. In this review, we summarized the recent literature, significant advancements, and challenges in the treatment and vaccination against brucellosis, with a special focus on developing countries.

show abstract

Section: Treatment Of Brucellosismentioning

confidence: 81%

Section: Introductionmentioning

confidence: 99%

An Overview of Brucellosis in Cattle and Humans, and its Serological and Molecular Diagnosis in Control Strategies

2018

View full text Add to dashboard Cite

show abstract

“…10 Despite efforts by DVS, several isolated cases of human brucellosis have been reported in the literature involving farmers, veterinarians, 11 and laboratory staff. 12,13 The occurrence of the disease is still low, however, as a study from 2004 to 2009 only found 10 (5.4%) of 184 suspected brucellosis cases to be seropositive. 11 Still, the true incidence of the disease could be higher as its nonspecific symptoms usually causes it to be underreported.…”

Section: Discussionmentioning

confidence: 99%

Outbreak of Human Brucellosis from Consumption of Raw Goats' Milk in Penang, Malaysia

Leong¹,

Chow²,

Wong³

et al. 2015

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. We report the largest outbreak of brucellosis in Penang, Malaysia. Brucellosis is not endemic in this region. The index case was a 45-year-old goat farm owner presented with 3 weeks of fever, headache, severe lethargy, poor appetite, and excessive sweating. He claimed to have consumed unpasteurized goat's milk that he had also sold to the public. Tests were negative for tropical diseases (i.e., dengue fever, malaria, leptospirosis and scrub typhus) and blood culture showed no growth. Based on epidemiological clues, Brucella serology was ordered and returned positive. Over a period of 1 year, 79 patients who had consumed milk bought from the same farm were diagnosed with brucellosis. Two of these patients were workers on the farm. Four laboratory staff had also contracted the disease presumably through handling of the blood samples. The mean duration from onset of symptoms to diagnosis was 53 days with a maximum duration of 210 days. A combination treatment of rifampin and doxycycline for 6 weeks was the first line of treatment in 90.5% of patients. One-third of the patients had sequelae after recovering and 21% had a relapse. We highlight the importance of Brucellosis as a differential diagnosis when a patient has unexplained chronic fever.

show abstract

“…In Malaysia, occupations associated with animals or animal related materials such as livestock workers, veterinarians and laboratory workers are most likely to be infected by brucellosis [ 23 , 24 ]. Human brucellosis cases in Malaysia also occurred through consumption of unpasteurized goat milk [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Multiple-locus variable-number tandem-repeat analysis (MLVA) genotyping of human Brucella isolates in Malaysia

et al. 2015

View full text Add to dashboard Cite

BackgroundBrucellosis is one of the most common zoonotic diseases worldwide. It can cause acute febrile illness in human and is a major health problem. Studies in human brucellosis in Malaysia is limited and so far no genotyping studies has been done on Brucella isolates. The aim of the study was to determine the genetic diversity among Brucella species isolated from human brucellosis, obtained over a 6-year period (2009–2014).MethodsIn this study, the genotypic characteristics of 43 human Brucella melitensis isolates were analysed using multiple-locus variable-number tandem-repeat analysis (MLVA) which consisted of eight minisatellite loci (panel 1) and eight microsatellite loci; panels 2A (3 microsatellite loci) and panel 2B (5 microsatellite loci). Two human Brucella suis isolates were also investigated using the MLVA assay.ResultsUsing panel 1 (MLVA8), two genotypes namely genotype 43 and 44 were obtained from the 43 B. melitensis isolates. Using the combination of panels 1 and 2A loci (MLVA11), two genotypes were obtained while using the complete panels 1, 2A and 2B, nine genotypes were obtained. The polymorphisms in using the complete panels (MLVA16) were observed in three loci from panel 2B, which showed a diversity index higher than 0.17. All B. melitensis isolates were closely related to the East Mediterranean group. For B. suis isolates, only genotype 6 and genotype 33 were obtained using panel 1 and MLVA11 respectively.ConclusionIn conclusion, the results of the present study showed a low genetic diversity among B. melitensis and B. suis isolates from human patients. Based on the MLVA16 assay, B. melitensis belonging to the East Mediterranean group is responsible for the vast majority of Brucella infections in our Malaysian patients. To our knowledge, this is the first genotyping study of human Brucella isolates in Malaysia.

show abstract

Clinical human brucellosis in Malaysia: a case report

Cited by 19 publications

References 17 publications

An Overview of Brucellosis in Cattle and Humans, and its Serological and Molecular Diagnosis in Control Strategies

An Overview of Brucellosis in Cattle and Humans, and its Serological and Molecular Diagnosis in Control Strategies

Outbreak of Human Brucellosis from Consumption of Raw Goats' Milk in Penang, Malaysia

Multiple-locus variable-number tandem-repeat analysis (MLVA) genotyping of human Brucella isolates in Malaysia

Contact Info

Product

Resources

About