Advanced microRNA-based cancer diagnostics using amplified time-gated FRET

Qiu, Xue; Xu, Jingyue; Guo, Jiajia; Yahia-Ammar, Akram; Kapetanakis, Nikiforos-Ioannis; Duroux-Richard, Isabelle; Unterluggauer, Julia Judith; Golob-Schwarzl, Nicole; Regeard, Christophe; Uzan, Catherine; Gouy, Sébastien; DuBow, Michael S.; Haybaeck, Johannes; Apparailly, Florence; Busson, P.; Hildebrandt, Niko

doi:10.1039/c8sc03121e

Cited by 38 publications

(60 citation statements)

References 45 publications

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“…A new technique for the detection and quantification of miRs using isothermally amplified time-gated Förster resonance energy transfer (TG-FRET) that has shown interest in breast and ovarian cancer could overcome these drawbacks in EC [85].…”

Section: Discussionmentioning

confidence: 99%

The Use of microRNAs in the Management of Endometrial Cancer: A Meta-Analysis

Delangle

Foucher

Larsen

et al. 2019

Cancers

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Introduction: Endometrial cancer (EC) is the most important gynecological cancer in terms of incidence. microRNAs (miRs), which are post-transcriptional regulators implicated in a variety of cellular functions including carcinogenesis, are particularly attractive candidates as biomarkers. Indeed, several studies have shown that the miR expression pattern appears to be associated with prognostic factors in EC. Our objective is to review the current knowledge of the role of miRs in carcinogenesis and tumor progression and their association with the prognosis of endometrial cancer. Materials and Method: We performed a literature search for miR expression in EC using MEDLINE, PubMed (the Internet portal of the National Library of Medicine) and The Cochrane Library, Cochrane databases “Cochrane Reviews” and “Clinical Trials” using the following keywords: microRNA, endometrial cancer, prognosis, diagnosis, lymph node, survival, plasma, FFPE (formalin-fixed, paraffin-embedded). The miRs were classified and presented according to their expression levels in cancer tissue in relation to different prognostic factors. Results: Data were collected from 74 original articles and 8 literature reviews which described the expression levels of 261 miRs in ECs, including 133 onco-miRs, 110 miR onco-suppressors, and 18 miRs with discordant functions. The review identified 30 articles studying the expression pattern of miR in neoplastic endometrial tissue compared to benign and/or hyperplastic tissues, 12 articles detailing the expression profile of miRs as a function of lymph node status, and 14 articles that detailed the expression pattern of miRs in endometrial tumor tissue according to overall survival or in the absence of recurrence. Conclusions: The findings presented here suggest that miR analysis merits a role as a prognostic factor in the management of patients with endometrial cancer.

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Section: Discussionmentioning

confidence: 99%

The Use of microRNAs in the Management of Endometrial Cancer: A Meta-Analysis

Delangle

Foucher

Larsen

et al. 2019

Cancers

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“…Accurate identication of cancer at an early stage plays an important role in cancer diagnosis and treatment. MicroRNAs (miRNAs) are a kind of cancer biomarker; their aberrant expression levels are closely related to the initiation and progression of cancers, [1][2][3][4] and therefore, sensitive detection of tumor related miRNAs holds great promise for cancer diagnostics and prognostics. [5][6][7][8] Furthermore, simultaneous detection of multiple tumor related miRNAs can avoid false-positive signals and enhance the accuracy of cancer diagnosis.…”

Section: Introductionmentioning

confidence: 99%

Accurate cancer cell identification and microRNA silencing induced therapy using tailored DNA tetrahedron nanostructures

Xia

et al. 2020

Chem. Sci.

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“…Owing to technical limitations of PCR techniques, such as relatively low CONTACT Niko Hildebrandt niko.hildebrandt@u-psud.fr NanoBioPhotonics (nanofret.com), Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, Université Paris-Sud, CNRS, CEA, 91405 Orsay Cedex, France specificity, rather extensive guidelines to obtain reliable results, and proprietary primer sequences for commercial kits, FRET has also been applied to many other DNA amplification technologies. Isothermal amplification methods, such as rolling circle amplification (RCA) [11] and hybridization chain reaction (HCR) [12], and hairpin-mediated quadratic isothermal amplification [13], are an ideal match for FRET because they also simplify the assay procedure and can be applied for cellular imaging. SNiPer genotyping technology (Amersham Pharmacia Biotech) is a FRET-based RCA detection method.…”

Section: Real-time Pcrmentioning

confidence: 99%

“…The discovery of many different miRNAs specific for breast, colorectal, esophageal, gastric, liver, lung, ovarian, and pancreatic cancers, have strongly driven the implementation of miRNAs as potential cancer biomarkers [32]. FRET probes have been largely explored in reverse transcription quantitative PCR (RT-qPCR) and new isothermal amplification strategies, such as RCA and HCR to perform single or multiplexed miRNA detections [11,12].…”

Section: Mirnasmentioning

confidence: 99%