Advanced Innovative Tools in Lemon (Citrus limon L.) Breeding

Polat, İlknur

doi:10.1007/978-3-319-91944-7_12

Cited by 4 publications

(3 citation statements)

References 102 publications

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“…One of them, "Eylul" lemon, showed no disease symptoms after controlled inoculation with P. tracheiphila [96], but long field trial performance of this new mutant has not been published yet. Recent advances in genomic science and molecular biology techniques will support in the development of faster, targeted and more efficient tools to expedite lemon breeding [97]. approach is to recover disease-resistant plants by selecting cellular lines resistant to a toxin produced by the pathogen [98].…”

Section: Biotechnology Approach To Develop and Select Msd Tolerant/rementioning

confidence: 99%

An Overview on Citrus Mal Secco Disease: Approaches and Strategies to Select Tolerant Genotypes in C. limon

2019

Crop Breed Genet Genom.

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Citrus is an economically important fruit crop that is seriously afflicted by several biotic stress, caused by both viruses and fungi, thus, new sustainable strategies to manage these diseases are needed. Lemon (Citrus limon (L.) Burm. f) is a tree in the Rutaceae family and after orange and mandarin, is the third most important Citrus species. Lemon fruits contain important bio-compounds and have central role in prevention of diseases of a balanced diet. Among C. limon diseases, "mal secco" is a highly destructive tracheomycotic fungal infection spread over the coasts of the Mediterranean basin and the Black Sea areas. The causal agent is the mitosporic fungus Phoma tracheiphila (Petri) Kantschaveli & Gikachvili (syn. Deuterophoma tracheiphila Petri). The pressure of disease has led to a marked decline of the Mediterranean's lemon crop acreage in the last 30 years. The present review explores the state of art in the struggle against mal secco disease (MSD), summarizing main results obtained from both conventional and unconventional breeding approaches. In addition, the strategies used to study the mechanisms involved in P. tracheiphila/lemon interaction and the results obtained to isolate key genes activates in tolerant genotypes are also described in depth. The overview of emergent approaches and technologies is focused to highlight the progress obtained using advanced lemon-breeding methods and suggest guidelines for future research.

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Section: Biotechnology Approach To Develop and Select Msd Tolerant/rementioning

confidence: 99%

An Overview on Citrus Mal Secco Disease: Approaches and Strategies to Select Tolerant Genotypes in C. limon

2019

Crop Breed Genet Genom.

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“…Furthermore, the edible rate of the smoothpeeled lemon fruits is greater (0.66) than that of the rough-peeled lemon fruits (0.44) (un published data). Such characteristics make the lemon fruits harvested in spring less attractive to customers (Sinanta, 2013;Polat, 2018;Klimek-Szczykutowicz et al, 2020). Particularly, the rough skin reduces its commodity value and, ultimately, causes losses to the farmers (Kaur et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Transcriptome and Metabolome Comparison of Smooth and Rough Citrus limon L. Peels Grown on Same Trees and Harvested in Different Seasons

Long

Wang

et al. 2021

Front. Plant Sci.

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Background: Farmers harvest two batches fruits of Lemons (Citrus limon L. Burm. f.) i.e., spring flowering fruit and autumn flowering fruit in dry-hot valley in Yunnan, China. Regular lemons harvested in autumn have smooth skin. However, lemons harvested in spring have rough skin, which makes them less attractive to customers. Furthermore, the rough skin causes a reduction in commodity value and economical losses to farmers. This is a preliminary study that investigates the key transcriptomic and metabolomic differences in peels of lemon fruits (variety Yuning no. 1) harvested 30, 60, 90, 120, and 150 days after flowering from the same trees in different seasons.Results: We identified 5,792, 4,001, 3,148, and 5,287 differentially expressed genes (DEGs) between smooth peel (C) and rough peel (D) 60, 90, 120, and 150 days after flowering, respectively. A total of 1,193 metabolites differentially accumulated (DAM) between D and C. The DEGs and DAMs were enriched in the mitogen-activated protein kinase (MAPK) and plant hormone signaling, terpenoid biosynthesis, flavonoid, and phenylalanine biosynthesis, and ribosome pathways. Predominantly, in the early stages, phytohormonal regulation and signaling were the main driving force for changes in peel surface. Changes in the expression of genes associated with asymmetric cell division were also an important observation. The biosynthesis of terpenoids was possibly reduced in rough peels, while the exclusive expression of cell wall synthesis-related genes could be a possible reason for the thick peel of the rough-skinned lemons. Additionally, cell division, cell number, hypocotyl growth, accumulation of fatty acids, lignans and coumarins- related gene expression, and metabolite accumulation changes were major observations.Conclusion: The rough peels fruit (autumn flowering fruit) and smooth peels fruit (spring flowering fruit) matured on the same trees are possibly due to the differential regulation of asymmetric cell division, cell number regulation, and randomization of hypocotyl growth related genes and the accumulation of terpenoids, flavonoids, fatty acids, lignans, and coumarins. The preliminary results of this study are important for increasing the understanding of peel roughness in lemon and other citrus species.

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“…Molecular markers have already been used for genetic characterization and improvement of many crop species. Recently, molecular and conventional breeding techniques are used together to obtain new varieties (Gentile and La Malfa, 2019).Molecular marker techniques are based on different principles, but they all can contribute to successfully identify the genome-wide variability (Polat, 2018). In this study, simple sequence repeats (SSR), inter-simple sequence repeat (ISSR) and single-strand conformation polymorphism (SSCP) molecular markers were used to identify the genetic differences in selected mutant citrus genotypes and in reference varieties.SSCP technique is easy and efficient to detect any small alteration in a PCRamplified product (Yadav and Kale, 2009).SSCP is originally used for rapid mutation analysis and has advantages on mutation detection (Değirmenci and Kunter, 2008).…”

mentioning

confidence: 99%

Identification of genetic diversity among mutant lemon and mandarin varieties using different molecular markers

Sülü¹,

Kaçar²,

Polat³

et al. 2020

Turk J Agric For

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Mandarin (Citrus reticulate Blanco) and lemon (C.limon L.) are among the most cultivated Citrus species in the world.Among different approaches, gamma radiation is widely used to induce mutations in citrus breeding studies. Gamma radiation causes DNA damage, randomly inducing several mutations in the genome. Molecular techniquesarewidely used to detect such induced mutations and genetic diversity in plants.In this study, simple sequence repeats (SSR), inter-simple sequence repeat (ISSR) and single-strand conformation polymorphism (SSCP) markers were used to detectthe induced mutations andgenetic diversityin someirradiated mandarin and lemon genotypes in comparison with standard citrus varieties. The irradiated genotypes used in this study derived from populationsthat had been originally obtained with gamma radiation with different gray doses applied on Yerli Mandarin and Antalya YerliYuvarlak lemon varieties. Out of 82 primers (22 SSCP, 30 ISSR and 30 SSR) tested, 55 primers (9 SSCP, 24 ISSR and 22 SSR primers) successfully amplified a total number of 363 amplicons.A single band with an approximate size of 500 nucleotides was determined using the primer ISSR 868 as differentiating between seedy parent and irradiated seedless mandarin genotypes. The polymorphism information content (PIC) values of SSCP, ISSR, and SSR markers ranged from 0.61-0.99, 0.31-0.96, and 0.33-0.96, respectively. Cluster analysis classified lemons and mandarins into 2 subgroups as mutant and commercial lemon genotypes. ISSR markers were found to be more effective to determine the genetic differences among the varieties in comparison to SSCP and SSR markers. The results clearly showed that SSCP, SSR and ISSR markers are important tools to distinguish mutant genotypes andconfirmed their usefulness for phylogenetic studies.

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Advanced Innovative Tools in Lemon (Citrus limon L.) Breeding

Cited by 4 publications

References 102 publications

An Overview on Citrus Mal Secco Disease: Approaches and Strategies to Select Tolerant Genotypes in C. limon

An Overview on Citrus Mal Secco Disease: Approaches and Strategies to Select Tolerant Genotypes in C. limon

Transcriptome and Metabolome Comparison of Smooth and Rough Citrus limon L. Peels Grown on Same Trees and Harvested in Different Seasons

Identification of genetic diversity among mutant lemon and mandarin varieties using different molecular markers

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