Transcriptome and Metabolome Comparison of Smooth and Rough Citrus limon L. Peels Grown on Same Trees and Harvested in Different Seasons

Li, Hongming; Long, Chun-Rui; Wang, Shaohua; Fu, Xiaomeng; Zhou, Xin; Mao, Jia-mei; Yang, Hongxia; Du, Yanxiu; Li, Jinxue; Jian-qiang, Yue; Hu, Faguang

doi:10.3389/fpls.2021.749803

Cited by 3 publications

(1 citation statement)

References 58 publications

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“…Eight samples of the pulp tissue of HP and HPP across four developmental stages were harvested, and three biological replicates were harvested for each sample. A total of 24 transcriptome profiles were obtained by RNA-seq using the Illumina HiSeq™ 4000 sequencing platform at Novogene (Beijing, China) ( Liu et al., 2021 ).…”

Section: Methodsmentioning

confidence: 99%

Transcriptome analysis of Harumi tangor fruits: Insights into interstock-mediated fruit quality

Liao

et al. 2022

Front. Plant Sci.

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Harumi tangor fruit with Ponkan as an interstock contains significantly higher levels of total soluble solids compared to Harumi tangor fruit cv.with no interstock. Transcriptome analysis of two graft combinations (Harumi/Hongjv (HP) and cv. cv.Harumi/Ponkan/Hongjv (HPP)) was conducted to identify the genes related to use of the Ponkan interstock. Soluble sugars and organic acids were also measured in the two graft combinations. The results showed that the contents of sucrose, glucose, and fructose were higher in the fruits of HPP than in those of HP; additionally, the titratable acid levels were lower in grafts with interstocks than in grafts without interstocks. Transcriptome analysis of HPP and HP citrus revealed that the interstock regulated auxin and ethylene signals, sugar and energy metabolism, and cell wall metabolism. Trend and Venn analyses suggested that genes related to carbohydrate-, energy-, and hormone-metabolic activities were more abundant in HPP plants than in HP plants during different periods. Moreover, weighted gene co-expression network analysis demonstrated that carbohydrates, hormones, cell wall, and transcription factors may be critical for interstock-mediated citrus fruit development and ripening. The contents of ethylene, auxin, cytokinin, transcription factors, starch, sucrose, glucose, fructose, and total sugar in HPP plants differed considerably than those in HP fruits. Interstocks may help to regulate the early ripening and quality of citrus fruit through the above-mentioned pathways. These findings provide information on the effects of interstock on plant growth and development.

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Section: Methodsmentioning

confidence: 99%

Transcriptome analysis of Harumi tangor fruits: Insights into interstock-mediated fruit quality

Liao

et al. 2022

Front. Plant Sci.

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Dynamics of ribosome composition and ribosomal protein phosphorylation in immune signaling in Arabidopsis thaliana

Siodmak,

Martinez-Seidel,

Rayapuram

et al. 2023

Nucleic Acids Research

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In plants, the detection of microbe-associated molecular patterns (MAMPs) induces primary innate immunity by the activation of mitogen-activated protein kinases (MAPKs). We show here that the MAMP-activated MAPK MPK6 not only modulates defense through transcriptional regulation but also via the ribosomal protein translation machinery. To understand the effects of MPK6 on ribosomes and their constituent ribosomal proteins (RPs), polysomes, monosomes and the phosphorylation status of the RPs, MAMP-treated WT and mpk6 mutant plants were analysed. MAMP-activation induced rapid changes in RP composition of monosomes, polysomes and in the 60S ribosomal subunit in an MPK6-specific manner. Phosphoproteome analysis showed that MAMP-activation of MPK6 regulates the phosphorylation status of the P-stalk ribosomal proteins by phosphorylation of RPP0 and the concomitant dephosphorylation of RPP1 and RPP2. These events coincide with a significant decrease in the abundance of ribosome-bound RPP0s, RPP1s and RPP3s in polysomes. The P-stalk is essential in regulating protein translation by recruiting elongation factors. Accordingly, we found that RPP0C mutant plants are compromised in basal resistance to Pseudomonas syringae infection. These data suggest that MAMP-induced defense also involves MPK6-induced regulation of P-stalk proteins, highlighting a new role of ribosomal regulation in plant innate immunity.

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Peel Essential Oil Composition and Antibacterial Activities of Citrus x sinensis L. Osbeck ‘Tarocco’ and Citrus reticulata Blanco

Zhang

Liú

et al. 2022

Horticulturae

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Oranges and mandarins in Chongqing, China, are mostly processed for juice and their peels are wasted or landfilled. To add value, it is essential to utilize the peels and extract useful materials, such as citrus essential oils (CEOs). Here, we report the metabolome analysis of the peels of Citrus x sinensis (CS) and Citrus reticulata (CR). In total, 793 metabolites were detected in the CS and CR peels, of which 242 were differentially accumulated. The metabolites were grouped into 12 compound classes. Terpenoids were the highest accumulated class of compounds followed by phenols and alcohols in both fruit peels. CR peels were rich in all types of compounds, whereas CS showed a relatively lower content of the detected compounds. The highest accumulated compounds were β-phellandrene, beta-ocimene, 1,3,6-octatriene,3,7-dimethyl, and d-limonene. Overall, CR showed higher compound diversity than CS. The CS and CR peel extracts showed antibacterial effects against Escherichia coli, Staphylococcus aureus, and Bacillus subtilis. The peel extracts from CR and CS showed similar antibacterial effects against E. coli and B. subtilis, while CS peel extracts were more effective against S. aureus. Overall, our study concludes that both CS and CR peels should not be wasted owing to the CEOs and respective antibacterial activities.

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Transcriptome and Metabolome Comparison of Smooth and Rough Citrus limon L. Peels Grown on Same Trees and Harvested in Different Seasons

Cited by 3 publications

References 58 publications

Transcriptome analysis of Harumi tangor fruits: Insights into interstock-mediated fruit quality

Transcriptome analysis of Harumi tangor fruits: Insights into interstock-mediated fruit quality

Dynamics of ribosome composition and ribosomal protein phosphorylation in immune signaling in Arabidopsis thaliana

Peel Essential Oil Composition and Antibacterial Activities of Citrus x sinensis L. Osbeck ‘Tarocco’ and Citrus reticulata Blanco

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