Advanced glycation end products in hemodialysates as fluorescent and optical absorption markers of patients mortality

Abstract

Hemodialysate (HD) samples collected from the end stage renal disease patients (ESRD Pts) were used for search for possible correlation between the intensity of HD visible auto-fluorescence (VF) detected at 420 nm as well as their optical absorption at 320 nm and the mortality events among the Pts. Previous but strongly promising correlations has been found in both cases which deserve further supplementation and examination. Investigation of possible influence of quenchers onto the VF intensity has been carried out. Endogenous inorganic ions present in biological fluids (serum, urine and HD) (Na, K, Ca, Mg and ammonia) do not affect the VF intensity remarkably but exogenous Al ions do that indirectly and specifically. Carbon based entities (nanoparticles of graphene type, humins) quench the VF effectively according to the Stern-Volmer law. The quenching phenomena and influence of aluminium must be taken into account by the further investigations, medical care and nutrition.

Visible fluorescence of biological fluids as a renal failure marker: New integrative approach

“…1,5 It has also been observed that the visible°uorescence (VF) intensity in hemodialysates correlates with typically high mortality index among ESRD patients and could be remarkably higher if measured in the hemodialysates of the ESRD patients who died later on. 6 The same phenomenon can be traced also in the hemodialysate speci¯c absorption measurements. 6 The monitoring is going on.…”

“…6 The same phenomenon can be traced also in the hemodialysate speci¯c absorption measurements. 6 The monitoring is going on.…”