Advanced backcross QTL analysis for the identification of quantitative trait loci alleles from wild relatives of wheat (Triticum aestivum L.)

Abstract

Advanced backcross QTL (AB-QTL) analysis was used to identify quantitative trait loci (QTLs) for yield and yield components in a BC(2)F(2) population derived from a cross between the German winter wheat variety 'Prinz' and the synthetic wheat line W-7984 developed by CIMMYT. Two hundred and ten microsatellite markers were employed to genotype 72 pre-selected BC(2)F(2) plants and phenotypic data were collected for five agronomic traits from corresponding BC(2)F(3) families that were grown at four locations in Germany. Using single-marker regression and interval mapping, a total of 40 putative QTLs derived from W-7984 were detected, of which 11 were for yield, 16 for yield components, eight for ear emergence time and five for plant height. For 24 (60.0%) of them, alleles from the synthetic wheat W-7984 were associated with a positive effect on agronomic traits, despite the fact that synthetic wheat was overall inferior with respect to agronomic appearance and performance. The present study indicated that favorable QTL alleles could be transferred from wild relatives of wheat into an elite wheat variety for improvement of quantitative trait loci like yield by the advanced backcross QTL strategy and molecular breeding. To our knowledge, the results presented here were the first report on AB-QTL analysis in wheat.

AB-QTL analysis in winter wheat: I. Synthetic hexaploid wheat (T. turgidum ssp. dicoccoides × T. tauschii) as a source of favourable alleles for milling and baking quality traits

“…Altogether four AB-QTL analyses of wheat have been published so far (Huang et al 2003(Huang et al , 2004Narasimhamoorthy et al 2006;Liu et al 2006). In all cases, the synthetic exotic donor parent originated from hybridisation of durum with T. tauschii, thus lacking exotic alleles on genomes A and B.…”

“…Synthetics derived from cultivated durum (T. turgidum ssp. durum) have been used in QTL studies (Nelson et al 1995;Mujeeb-Kazi et al 1996;Börner et al 2002;Huang et al 2003Huang et al , 2004Nelson et al 2006). Synthetics derived from tetraploid wild emmer (T. turgidum spp.…”

AB-QTL analysis in spring barley: II. Detection of favourable exotic alleles for agronomic traits introgressed from wild barley (H. vulgare ssp. spontaneum)

“…Since QTL detection is carried out in advanced backcross populations, problems associated with considerable phenotypic variation and linkage drag in interspecific crosses are reduced. So far, several reports on the application of the AB-QTL strategy are available for tomato Fulton et al 1997Fulton et al , 2000Fulton et al , 2002Bernacchi et al 1998); rice (Xiao et al 1996(Xiao et al , 1998Moncada et al 2001;Brondani et al 2002;Septiningsih et al 2003;Wu et al 2004); maize (Ho et al 2002); pepper (Rao et al 2003); wheat (Huang et al 2003andbarley (Pillen et al 2003, 2004;Matus et al 2003;Li et al 2004). The favourable effects of the wild barley accession ISR101-23 on yield in two barley feeding varieties recorded by Pillen et al (2003Pillen et al ( , 2004 encourage further investments in the development of AB-populations for the genetic exploitation of diverse exotic barley germplasms.…”

Molecular dissection of plant height QTLs using recombinant inbred lines from hybrids between common wheat (Triticum aestivum L.) and spelt wheat (Triticum spelta L.)

“…Some of these genes have been *Corresponding authors (email: penghuiru@cau.edu.cn; qxsun@cau.edu.cn) exploited to breed many semidwarf cultivars with high yield potential. Recently, along with the advent of molecular quantitative genetics, about 50 quantitative trait loci (QTLs) for wheat plant height have been identified using the constructed molecular genetic linkage maps [3][4][5][6][7][8][9][10][11][12][13][14][15]. However, QTL mapping of plant height in wheat was only focused on the performance of this trait at the maturing stage.…”