AB-QTL analysis in spring barley: II. Detection of favourable exotic alleles for agronomic traits introgressed from wild barley (H. vulgare ssp. spontaneum)

Abstract

The objective of the present study was to identify favourable exotic Quantitative Trait Locus (QTL) alleles for the improvement of agronomic traits in the BC 2 DH population S42 derived from a cross between the spring barley cultivar Scarlett and the wild barley accession ISR42-8 (Hordeum vulgare ssp. spontaneum). QTLs were detected as a marker main effect and/or a marker · environment interaction effect (M · E) in a three-factorial ANOVA. Using field data of up to eight environments and genotype data of 98 SSR loci, we detected 86 QTLs for nine agronomic traits. At 60 QTLs the marker main effect, at five QTLs the M · E interaction effect, and at 21 QTLs both the effects were significant. The majority of the M · E interaction effects were due to changes in magnitude and are, therefore, still valuable for marker assisted selection across environments. The exotic alleles improved performance in 31 (36.0%) of 86 QTLs detected for agronomic traits. The exotic alleles had favourable effects on all analysed quantitative traits. These favourable exotic alleles were detected, in particular on the short arm of chromosome 2H and the long arm of chromosome 4H. The exotic allele on 4HL, for example, improved yield by 7.1%. Furthermore, the presence of the exotic allele on 2HS increased the yield component traits ears per m 2 and thousand grain weight by 16.4% and 3.2%, respectively. The present study, hence, demonstrated that wild barley does harbour valuable alleles, which can enrich the genetic basis of cultivated barley and improve quantitative agronomic traits.

QTL analysis in Barley Across Environments in Egypt

“…Only on QTL showed both effect with desirable increase in GYP due to the presence of the exotic alleles. This results are in harmony with those obtained by (Pillen et al, 2003 ;2004;von Korff et al 2006 ;Wang et al, 2010 andSayed, 2011). We detected one marker (bpb_3278 on 3H) which revealed marker main and MEI effects.…”

“…The exotic alleles of this locus was responsible for increasing this trait. Nine QTLs were responsible for 1000-GW and mapped on all chromosomes except 1H (von Korff et al, 2006).…”

AB-QTL analysis in winter wheat: I. Synthetic hexaploid wheat (T. turgidum ssp. dicoccoides × T. tauschii) as a source of favourable alleles for milling and baking quality traits

“…For QTL detection, the following mixed hierarchical model described by von Korff et al (2006) was applied:…”

“…The relative performance of the homozygous exotic genotype (RP[aa]) was calculated as described by Pillen et al (2003). The genetic variance explained by a marker (R 2 M ) was calculated as described by von Korff et al (2006). The proportion of the exotic genome (P[exot]) was calculated as the percentage of exotic alleles present in a single BC 2 F 3 line according to the formula: …”

Molecular detection of QTL controlling plant height components in a doubled haploid barley population

“…In addition to the semi-dwarfing and dwarfing genes, a number of quantitative trait loci (QTL) are also known to affect plant height in barley. QTL conferring plant height are reported to be located on all seven chromosomes (Backes et al, 1995;Kjaer et al, 1995;Hori et al, 2003;Pillen et al, 2003;Sameri et al, 2006;von Korff et al, 2006;Baghizadeh et al, 2007;Wang et al, 2010).…”