Advanced application of bovine intestinal epithelial cell line for evaluating regulatory effect of lactobacilli against heat-killed enterotoxigenic Escherichia coli-mediated inflammation

Takanashi, Naoya; Tomosada, Yohsuke; Villena, Julio; Murata, Keiju; Takahashi, Takuya; Chiba, Eriko; Tohno, Masanori; Shimazu, Tomoyuki; Aso, Hisashi; Suda, Yoshihito; Ikegami, Shuji; Itoh, Hiroyuki; Kawai, Yasushi; Saito, Tadao; Álvarez, Susana; Kitazawa, Haruki

doi:10.1186/1471-2180-13-54

Cited by 46 publications

(53 citation statements)

References 34 publications

(55 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The TLR-2 and TLR-4 activation and expression by LPS (pathogens) is known as one of the most important mechanisms by which the immune system controls reactions to bacteria in particular in the activation phase, therefore, over-expression of TLR-2 and TLR-4 during any bacterial infection could cause an elevated inflammatory response in the body. While early activation of TLRs expression is reported in response to bacterial LPS from pathogenic Salmonella typhimurium [61] as well as E. coli infection in bovine intestinal epithelial cells [66], our results show tolerance as a result of co-culturing PBMC with S. thermophilus 285 by down regulation of TLRs genes. Downregulated mRNA expression of TLRs genes, specifically TLR-1, TLR-2, TLR-4 and TLR-8 indicates anti-inflammatory characteristics for S. thermophilus 285.…”

Section: S Thermophilus 285 Downregulates Mrna Expression Levels Of contrasting

confidence: 63%

Streptococcus thermophilus alters the expression of genes associated with innate and adaptive immunity in human peripheral blood mononuclear cells

2020

View full text Add to dashboard Cite

Consumption of probiotics contributes to a healthy microbiome of the GIT leading to many health benefits. They also contribute to the modulation of the immune system and are becoming popular for the treatment of a number of immune and inflammatory diseases. The main objective of this study was to evaluate anti-inflammatory and modulatory properties of Streptococcus thermophilus. We used peripheral blood mononuclear cells from healthy donors and assessed modifications in the mRNA expression of their genes related to innate and adaptive immune system. Our results showed strong immune modulatory effects of S. thermophilus 285 to human peripheral blood mononuclear cells with an array of anti-inflammatory properties. S. thermophilus 285 reduced mRNA expression in a number of inflammatory immune mediators and markers, and upregulated a few of immune markers. S. thermophilus is used in the dairy industry, survives during cold storage, tolerates well upon ingesting, and their consumption may have beneficial effects with potential implications in inflammatory and autoimmune disorders.

show abstract

Section: S Thermophilus 285 Downregulates Mrna Expression Levels Of contrasting

confidence: 63%

Streptococcus thermophilus alters the expression of genes associated with innate and adaptive immunity in human peripheral blood mononuclear cells

2020

View full text Add to dashboard Cite

show abstract

“…Less clear is whether the negative regulators are the targets of pathogen infection. For instance, IRAK-M was up-regulated in LPS treated macrophages or in lung epithelial cells in response to S. pneumonia [50], [55], while ETEC infection did not modify IRAK-M and Tollip expression in bovine epithelial cells, and S. typhi caused a decrease in Tollip level in dendritic cells [26], [56]. Our results show a decrease in Tollip and IRAK-M levels triggered by ETEC infection in Caco-2/TC7 cells, whereas these negative regulators are unaffected by ETEC in intestinal explants.…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus amylovorus Inhibits the TLR4 Inflammatory Signaling Triggered by Enterotoxigenic Escherichia coli via Modulation of the Negative Regulators and Involvement of TLR2 in Intestinal Caco-2 Cells and Pig Explants

et al. 2014

View full text Add to dashboard Cite

Inflammation derived from pathogen infection involves the activation of toll-like receptor (TLR) signaling. Despite the established immunomodulatory activities of probiotics, studies relating the ability of such bacteria to inhibit the TLR signaling pathways are limited or controversial. In a previous study we showed that Lactobacillus amylovorus DSM 16698T, a novel lactobacillus isolated from unweaned pigs, protects the intestinal cells from enterotoxigenic Escherichia coli (ETEC) K88 infection through cytokine regulation. In the present study we investigated whether the ability of L. amylovorus to counteract the inflammatory status triggered by ETEC in intestine is elicited through inhibition of the TLR4 signaling pathway. We used the human intestinal Caco-2/TC7 cells and intestinal explants isolated from 5 week-old crossbreed Pietrain/Duroc/Large-White piglets, treated with ETEC, L. amylovorus or L. amylovorus cell free supernatant, either alone or simultaneously with ETEC. Western blot analysis showed that L. amylovorus and its cell free supernatant suppress the activation of the different steps of TLR4 signaling in Caco-2/TC7 cells and pig explants, by inhibiting the ETEC induced increase in the level of TLR4 and MyD88, the phosphorylation of the IKKα, IKKβ, IκBα and NF-κB subunit p65, as well as the over-production of inflammatory cytokines IL-8 and IL-1β. The immunofluorescence analysis confirms the lack of phospho-p65 translocation into the nucleus. These anti-inflammatory effects are achieved through modulation of the negative regulators Tollip and IRAK-M. We also found that L. amylovorus blocks the up-regulation of the extracellular heat shock protein (Hsp)72 and Hsp90, that are critical for TLR4 function. By using anti-TLR2 antibody, we demonstrate that TLR2 is required for the suppression of TLR4 signaling activation. These results may contribute to develop therapeutic interventions using L. amylovorus in intestinal disorders of piglets and humans.

show abstract

“…The SISP model also allowed determination of the early gene expression response of the pig small intestinal mucosa to infection with ETEC (28) and Salmonella (29). Most studies employing SISP, however, focused on analysis of physiological and immune responses of the host, including fluid loss, electrolyte secretion, and the expression of cytokines (23,26,27,30). Very few studies provided a detailed bacteriological analysis of the model; previous studies using the SISP model reported only cell counts of ETEC in the lumen (27,31,32).…”

Section: Discussionmentioning

confidence: 99%

Exopolysaccharides Synthesized by Lactobacillus reuteri Protect against Enterotoxigenic Escherichia coli in Piglets

Chen

Woodward

Zijlstra

et al. 2014

Appl Environ Microbiol

View full text Add to dashboard Cite

bEnterotoxigenic Escherichia coli (ETEC) is a major cause of diarrhea in piglets; ETEC cells colonize the intestinal mucosa with adhesins and deliver toxins that cause fluid loss. This study determined the antiadhesive properties of bacterial exopolysaccharides (reuteran and levan) and related glycans (dextran and inulin) in a small intestinal segment perfusion (SISP) model. The SISP model used 10 jejunal segments from 5-week-old piglets. Five segments were infected with ETEC expressing K88 fimbriae (ETEC K88), while five segments were treated with saline. Every two segments (ETEC and non-ETEC infected) were infused with 65 ml of 10 g liter ؊1 of glycans or saline (control) for 8 h. High-resolution melting-curve (HRM) quantitative PCR (qPCR) indicated that E. coli is the dominant bacterium in infected segments, while other bacteria were predominant in noninfected segments. Infection by ETEC K88 was also verified by qPCR; gene copy numbers of K88 fimbriae and the heat-labile toxin (LT) in mucosal scrapings and outflow fluid of infected segments were significantly higher than those in noninfected segments. Genes coding for K88 fimbriae and LT were also detected in noninfected segments. LT amplicons from infected and noninfected segments were 99% identical over 481 bp, demonstrating the presence of autochthonous ETEC K88. All glycans reduced fluid loss caused by ETEC K88 infection. Reuteran tended (P ‫؍‬ 0.06) to decrease ETEC K88 levels in mucosal scraping sample, as judged by qPCR. Fluorescent in situ hybridization analysis demonstrated that reuteran significantly (P ‫؍‬ 0.012) decreased levels of adherent ETEC K88. Overall, reuteran may prevent piglet diarrhea by reducing adhesion of ETEC K88.

show abstract

Advanced application of bovine intestinal epithelial cell line for evaluating regulatory effect of lactobacilli against heat-killed enterotoxigenic Escherichia coli-mediated inflammation

Cited by 46 publications

References 34 publications

Streptococcus thermophilus alters the expression of genes associated with innate and adaptive immunity in human peripheral blood mononuclear cells

Streptococcus thermophilus alters the expression of genes associated with innate and adaptive immunity in human peripheral blood mononuclear cells

Lactobacillus amylovorus Inhibits the TLR4 Inflammatory Signaling Triggered by Enterotoxigenic Escherichia coli via Modulation of the Negative Regulators and Involvement of TLR2 in Intestinal Caco-2 Cells and Pig Explants

Exopolysaccharides Synthesized by Lactobacillus reuteri Protect against Enterotoxigenic Escherichia coli in Piglets

Contact Info

Product

Resources

About