Adult Rat Spinal Cord Culture On An Organosilane Surface In A Novel Serum-Free Medium

Das, Mainak; Bhargava, Neelima; Gregory, Cassie; Riedel, Lisa; Molnár, Péter; Hickman, James J.

doi:10.1290/0505031

Cited by 13 publications

(42 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…First, a unique chemically defined serum-free medium supplemented with specific growth factors was developed to study the muscle differentiation process. Second, a synthetic, nonbiological, patternable 8,9 , cell growth promoting substrate, N-1 [3(trimethoxysilyl)propyl] diethylenetriamine (DETA), coated on glass coverslips [10][11][12] , was used as a template to grow the skeletal muscle cells. Third, it was demonstrated that when the dissociated muscle cells were plated on fabricated microcantilevers, they aligned along the long axis of the cantilever to form aligned myotubes.…”

Section: Introductionmentioning

confidence: 99%

Differentiation of skeletal muscle and integration of myotubes with silicon microstructures using serum-free medium and a synthetic silane substrate

et al. 2007

Self Cite

View full text Add to dashboard Cite

This protocol describes a cell culture model to study the differentiation of fetal rat skeletal muscle cells. The model uses serum-free medium, a nonbiological substrate N-1[3(trimethoxysilyl)propyl] diethylenetriamine (DETA) and fabricated microcantilevers to promote the differentiation of dissociated rat myocytes into robust myotubes. In this protocol, we also describe how to characterize the myotubes on the basis of morphology, immunocytochemistry and electrophysiology. Here, four major techniques are employed: fabrication of cantilevers, surface modification of the glass and cantilever substrates with a DETA SAM, a serum-free medium and refined culture techniques. This culture system has potential applications in biocompatibility studies, bioartificial muscle engineering, skeletal muscle differentiation studies and for better understanding of myopathies and neuromuscular disorders. The model can be established in 26-33 d.

show abstract

Section: Introductionmentioning

confidence: 99%

Differentiation of skeletal muscle and integration of myotubes with silicon microstructures using serum-free medium and a synthetic silane substrate

et al. 2007

Self Cite

View full text Add to dashboard Cite

show abstract

“…Fully functional in vitro model systems could be useful not only in spinal cord injury studies but possibly also for models of amyotrophic lateral sclerosis (ALS), multiple sclerosis (MS) and neuropathic pain. Recent advancements in the culture of adult mammalian spinal cord (Das et al, 2005;) and brain neurons (Brewer, 1999;Fedoroff and Richardson, 2001;Brewer, 1997) in a completely defined serum-free medium, suggest outstanding potential for answering questions that relate to maturation, aging, neurodegeneration and injury, as well as the ability to screen different novel and putative drug candidates for CNS repair and degenerative diseases of the CNS. Prominent features of the survival of adult CNS neurons in these culture systems have been ascribed to a permissive growth promoting substrate and defined culture medium (Das et al, 2005;Brewer, 1999;Fedoroff and Richardson, 2001;Brewer, 1997).…”

Section: Introductionmentioning

confidence: 99%

“…However, there are few reports on the evaluation of the electrical functionality and regeneration of adult CNS neurons in long-term culture (Das et al, 2005;Brewer, 1997;Evans et al, 1998). Recent electrophysiological studies on adult spinal cord neurons in a defined culture indicated that only approximately 30% of the total surviving neurons were electrically active (Das et al, 2005;.…”

Section: Introductionmentioning

confidence: 99%

“…Recent electrophysiological studies on adult spinal cord neurons in a defined culture indicated that only approximately 30% of the total surviving neurons were electrically active (Das et al, 2005;. In all cases, the neurons showed a very weak inward and outward current in voltage clamp studies and only fired single action potentials with limited culture duration (Das et al, 2005;.…”

Section: Introductionmentioning

confidence: 99%

“…VEGF (Azzouz et al, 2004;Lambrechts et al, 2003), G5 supplement (Bottenstein, 1985), NT-4 (Bregman et al, 1997;Friedman et al, 1995) and CNTF (Kato and Lindsay, 1994;Masu et al, 1993;Oppenheim et al, 1991)) to a previously developed model (Das et al, 2005;. In addition, it was discovered that the electrical functionality of 60% of the neurons could be re-established by long-term temporal incubation of the cultures with serotonin + glutamate (N-acetyl-DLglutamic acid) followed by acetylcholine-chloride, providing in vitro evidence to support the hypothesis that extracellular neurotransmitters may be involved in shaping synaptic circuits in vivo.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Temporal neurotransmitter conditioning restores the functional activity of adult spinal cord neurons in long-term culture

Das

Bhargava

Bhalkikar

et al. 2008

Experimental Neurology

Self Cite

View full text Add to dashboard Cite

The ability to culture functional adult mammalian spinal-cord neurons represents an important step in the understanding and treatment of a spectrum of neurological disorders including spinal cord injury. Previously, the limited functional recovery of these cells, as characterized by a diminished ability to initiate action potentials and to exhibit repetitive firing patterns, has arisen as a major impediment to their physiological relevance. In this report we demonstrate that single temporal doses of the neurotransmitters serotonin, glutamate (N-acetyl-DL-glutamic acid) and acetylcholinechloride leads to the full electrophysiological functional recovery of adult mammalian spinal-cord neurons, when they are cultured under defined serum-free conditions. Approximately 60% of the neurons treated regained their electrophysiological signature, often firing single, double and, most importantly, multiple action potentials.

show abstract

Inverse liquid‐solid chromatography to evaluate drug interactions with organosilane‐modified polydimethylsiloxane for use in body‐on‐a‐chip systems

Schnepper

Roles

Hickman

2020

Biotechnology Progress

View full text Add to dashboard Cite

Body-on-a-chip and organ-on-a-chip systems utilize polydimethylsiloxane (PDMS) because of the relative suitability of the material for fabrication of microfluidic channels and chambers used in these devices. However, hydrophobic molecules, especially therapeutic compounds, tend to adsorb to PDMS, which may distort the doseresponse curves that feed into the pharmacokinetic/pharmacodynamic models used to translate preclinical data into predictions of clinical outcomes. Surface modification by organosilanes is one method being explored to modify PDMS, but the effect of organosilanes on drug adsorption isotherms is not well characterized. We utilized Inverse Liquid-Solid Chromatography to characterize the adsorption parameters of the drugs acetaminophen, diclofenac, and verapamil with native PDMS and organosilane-modified (fluoropolymer (13F) and polyethylene glycol) PDMS surfaces, to correlate the modifications with changes in drug adsorption. It was determined that the organosilane modifications significantly changed the energy of adsorption of the test drug utilizing our methodology.

show abstract

Adult Rat Spinal Cord Culture On An Organosilane Surface In A Novel Serum-Free Medium

Cited by 13 publications

References 0 publications

Differentiation of skeletal muscle and integration of myotubes with silicon microstructures using serum-free medium and a synthetic silane substrate

Differentiation of skeletal muscle and integration of myotubes with silicon microstructures using serum-free medium and a synthetic silane substrate

Temporal neurotransmitter conditioning restores the functional activity of adult spinal cord neurons in long-term culture

Inverse liquid‐solid chromatography to evaluate drug interactions with organosilane‐modified polydimethylsiloxane for use in body‐on‐a‐chip systems

Contact Info

Product

Resources

About