Adult Opisthorchis felineus major protein fractions deduced from transcripts: Comparison with liver flukes Opisthorchis viverrini and Clonorchis sinensis

Pomaznoy, Mikhail; Tat'kov, S. I.; Katokhin, Alexey V.; Afonnikov, D. A.; Babenko, Vladimir N.; Furman, D. P.; Brusentsov, Ilya I.; Belavin, P. A.; Najakshin, Alexandr; Guselnikov, Sergey; Vasiliev, Gennady V.; Sivkov, Anton; Prokhortchouk, Egor; Skryabin, K. G.; Mordvinov, Viatcheslav A.

doi:10.1016/j.exppara.2013.07.011

Cited by 15 publications

(6 citation statements)

References 56 publications

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“…In experiments on F. hepatica it was shown that these enzymes have prostaglandin-synthase activity 57 . Previously, we have shown that O. felineus GST has a high affinity for GST from related trematodes species 58 . A recent preclinical assessment of stem of Nicotiana tabacum on excision wound model demonstrated high levels of GST in wound area, which was associated with a successful wound healing process 59 .…”

Section: Discussionmentioning

confidence: 94%

Wound healing approach based on excretory-secretory product and lysate of liver flukes

Ковнер

Tarasenko

Zaparina

et al. 2022

Sci Rep

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Exogenous bioactive peptides are considered promising for the wound healing therapy in humans. In this regard, parasitic trematodes proteins may potentially become a new perspective agents. Foodborne trematode Opisthorchis felineus is widespread in Europe and has the ability to stimulate proliferation of bile duct epithelium. In this study, we investigated skin wound healing potential of O. felineus proteins in mouse model. C57Bl/6 mice were inflicted with superficial wounds with 8 mm diameter. Experimental groups included several non-specific controls and specific treatment groups (excretory-secretory product and lysate). After 10 days of the experiment, the percentage of wound healing in the specific treatment groups significantly exceeded the control values. We also found that wound treatment with excretory-secretory product and worm lysate resulted in: (i) inflammation reducing, (ii) vascular response modulating, (iii) type 1 collagen deposition promoting dermal ECM remodeling. An additional proteomic analysis of excretory-secretory product and worm lysate samples was revealed 111 common proteins. The obtained data indicate a high wound-healing potential of liver fluke proteins and open prospects for further research as new therapeutic approaches.

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Section: Discussionmentioning

confidence: 94%

Wound healing approach based on excretory-secretory product and lysate of liver flukes

Ковнер

Tarasenko

Zaparina

et al. 2022

Sci Rep

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“…Several studies have comprehensively examined and characterized the tegument and ES proteomes from C. sinensis (Zheng et al, 2013;Wang et al, 2014;Shi et al, 2020), O. viverrini (Boonmee et al, 2003;Mulvenna et al, 2010), and O. felineus (Kovner et al, 2022). Despite its health implications, only one previous study sought to characterize the secreted proteome of O. felineus; most other studies have been more limited in their experimental scope and identified only small numbers of proteins (Pomaznoy et al, 2013;L'Vova et al, 2014;Pomaznoy et al, 2016). The transcriptomes of the adult and metacercaria stages of O. felineus were comprehensively elucidated in a study that generated 12,665 protein-encoding transcripts with Illumina sequencing (Pomaznoy et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Secreted and surface proteome and transcriptome of Opisthorchis felineus

Wong,

Pearson,

Fedorova

et al. 2023

Front. Parasitol.

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IntroductionOpisthorchis felineus, Opisthorchis viverrini, and Clonorchis sinensis are the most medically important species of fish-borne zoonotic trematodes. O. felineus is endemic to the river plains of Western Siberia and Eastern Europe, and it is estimated that more than 1.6 million people could be infected with this parasite. Chronic opisthorchiasis may lead to significant gastrointestinal and hepatobiliary pathology. This study aimed to identify and characterize proteins from the secreted and tegumental proteomes of O. felineus. MethodsAdult flukes were collected from experimentally infected hamsters and cultured in vitro in serum-free media. We extracted proteins from different compartments of the O. felineus secretome, including (i) soluble excretory/secretory (ES) products; (ii) secreted 15K-extracellular vesicles (EVs); and (iii) tegument. ResultsWe also generated a transcriptome using long-read sequencing, and when this was combined with high-resolution mass spectrometry, sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) separation, and protein digestion, we identified 686, 894, 389, 324, and 165 proteins from the ES, 15K-EV, and the three sequentially extracted tegument (TEG) protein fractions, respectively. We conducted in-depth gene ontology and protein family analyses on the identified proteins and discussed comparisons against similar proteome data sets acquired for the Southeast Asian liver fluke O. viverrini and the Chinese liver fluke C. sinensis. DiscussionThe information from this study will form a biologically relevant data set of O. felineus proteins that could be used to develop diagnostic and therapeutic tools to manage the human cost of O. felineus infection and its associated comorbidities.

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“…Draft genomes are published for both O. viverrini (634.5 Mb) [ 39 ] and O. felineus (684 Mb) [ 40 ]. Furthermore, there is a wealth of available data on the transcriptome/secretome and proteome of both species providing key information that can be used for identifying potential novel antigens for vaccine development [ 24 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 ]. Presumptive anti-worm and anti-fecundity vaccine candidates include glutathione S-transferase, other anti-oxidant/detoxification proteins, fatty acid binding protein, myoglobin, ferritin, various proteases (e.g., cathepsin F, B1), eggshell protein, SH3-domain containing protein, Ly6-domain containing protein, paramyosin, calreticulin and granulin-like growth factor (Ov-GRN-1) [ 41 , 44 , 48 ]; however, none appear to have been tested in the hamster vaccination model.…”

Section: Opisthorchiasismentioning

confidence: 99%

Recent Progress in the Development of Liver Fluke and Blood Fluke Vaccines

McManus

2020

Vaccines

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Liver flukes (Fasciola spp., Opisthorchis spp., Clonorchis sinensis) and blood flukes (Schistosoma spp.) are parasitic helminths causing neglected tropical diseases that result in substantial morbidity afflicting millions globally. Affecting the world’s poorest people, fasciolosis, opisthorchiasis, clonorchiasis and schistosomiasis cause severe disability; hinder growth, productivity and cognitive development; and can end in death. Children are often disproportionately affected. F. hepatica and F. gigantica are also the most important trematode flukes parasitising ruminants and cause substantial economic losses annually. Mass drug administration (MDA) programs for the control of these liver and blood fluke infections are in place in a number of countries but treatment coverage is often low, re-infection rates are high and drug compliance and effectiveness can vary. Furthermore, the spectre of drug resistance is ever-present, so MDA is not effective or sustainable long term. Vaccination would provide an invaluable tool to achieve lasting control leading to elimination. This review summarises the status currently of vaccine development, identifies some of the major scientific targets for progression and briefly discusses future innovations that may provide effective protective immunity against these helminth parasites and the diseases they cause.

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Adult Opisthorchis felineus major protein fractions deduced from transcripts: Comparison with liver flukes Opisthorchis viverrini and Clonorchis sinensis

Cited by 15 publications

References 56 publications

Wound healing approach based on excretory-secretory product and lysate of liver flukes

Wound healing approach based on excretory-secretory product and lysate of liver flukes

Secreted and surface proteome and transcriptome of Opisthorchis felineus

Recent Progress in the Development of Liver Fluke and Blood Fluke Vaccines

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