Adult derived mononuclear bone marrow cells improve survival in a model of acetaminophen-induced acute liver failure in rats

Bone Marrow Mononuclear Cell Transplantation Improves Survival and Induces Hepatocyte Proliferation in Rats after CCl4 Acute Liver Damage

“…Bone marrow is the main source of cells used for therapy. Studies using purified hematopoietic [4] and mesenchymal [5] fractions or mononuclear cells [6] have been shown to improve liver function after transplant.…”

Bone Marrow-Derived Mononuclear Cells Differentiate into Hepatocyte-Like Cells within Few Hrs without Fusion

“…BMMC were isolated from the femur and tibia of Wistar rats, as previously reported [1]. Briefly, bone marrow was flushed with Dulbecco's Modified Eagle Medium (DMEM-LGC, Brazil) supplemented with 10% Fetal Bovine Serum (FBS-Gibco, USA) and 1% Penicilin/Streptomicin (P/S-Gibco, USA) and BMMC were separated onto a Ficoll Histopaque (GE-Healthcare, USA) layer.…”

“…In models of acute liver failure, transplantation of these cells increase the survival rate [1,2]. Furthermore, recent clinical trials have demonstrated that transplantation of these cells or their fractions improves the condition of patients with cirrhosis [3][4][5], as well as improve liver function in animal models of cirrhosis [6].…”

Freshly isolated hepatocyte transplantation in acetaminophen-induced hepatotoxicity model in rats

“…An oral solution of APAP at 200 mg/ mL concentration (Medley, Brazil) was administered in a single dose of 1 g/kg via intraperitoneal injection in female rats weighing 200-350 g. Four days before the intraperitoneal injection, animals received phenobarbital (Fenocrisis 4%, Brazil) at 350 mg/L in drinking water to induce cytochrome P-450 enzymes to potentiate subsequent APAP-toxicity (2) . Phenobarbital was offered until the last day of the study (day 3).…”