Bone Marrow-Derived Mononuclear Cells Differentiate into Hepatocyte-Like Cells within Few Hrs without Fusion

Matte, Úrsula; Cruz, Carolina Uribe; López, Mónica Luján; Simon, Laura; Mayer, Fabiana Quoos; Giugliani, Roberto

doi:10.4172/2157-7013.1000163

Cited by 1 publication

(1 citation statement)

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“…Five naïve male Wistar rats were used as BMMC donors as described by Matte et al [11]. Briefly, in a sterile environment, the femurs and tibias were isolated and whole bone marrow was flushed with complete medium: DMEM (Dulbecco's Modified Eagle Medium, LGC, Brazil) supplemented with 10% fetal bovine serum (Gibco, USA), 1% penicillin/streptomycin (Gibco, USA) and centrifuged at 800 g for 5 minutes.…”

Section: Methodsmentioning

confidence: 99%

Paracrine Effects of Bone Marrow Mononuclear Cells in Survival and Cytokine Expression after 90% Partial Hepatectomy

Kieling

Cruz

López

et al. 2017

Stem Cells International

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Acute liver failure is a complex and fatal disease. Cell-based therapies are a promising alternative therapeutic approach for liver failure due to relatively simple technique and lower cost. The use of semipermeable microcapsules has become an interesting tool for evaluating paracrine effects in vivo. In this study, we aimed to assess the paracrine effects of bone marrow mononuclear cells (BMMC) encapsulated in sodium alginate to treat acute liver failure in an animal model of 90% partial hepatectomy (90% PH). Encapsulated BMMC were able to increase 10-day survival without enhancing liver regeneration markers. Gene expression of Il-6 and Il-10 in the remnant liver was markedly reduced at 6 h after 90% PH in animals receiving encapsulated BMMC compared to controls. This difference, however, was neither reflected by changes in the number of CD68+ cells nor by serum levels of IL6. On the other hand, treated animals presented increased caspase activity and gene expression in the liver. Taken together, these results suggest that BMMC regulate immune response and promote apoptosis in the liver after 90% PH by paracrine factors. These changes ultimately may be related to the higher survival observed in treated animals, suggesting that BMMC may be a promising alternative to treat acute liver failure.

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Section: Methodsmentioning

confidence: 99%