Adsorption of proteins involved in hydrolysis of lignocellulose on lignins and hemicelluloses

Pareek, Nidhi; Gillgren, Thomas; Jönsson, Leif J.

doi:10.1016/j.biortech.2013.08.121

Cited by 131 publications

(79 citation statements)

References 32 publications

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“…Alkali-treated lignin exhibited to a higher specific surface area and pore volume than the LignoBoost lignin, suggesting an increase in the pretreatment severity of the applied process conditions for the alkali lignin. In the meanwhile, the alkali lignin BET results interestingly were comparable to another study [21]. However, to the best of the authors' knowledge, the BET parameters for LignoBoost lignin has not been previously reported.…”

Section: Resultssupporting

confidence: 82%

Physicochemical Characterisation of Technical Lignins for Their Potential Valorisation

Abdelaziz

Hulteberg

2016

Waste Biomass Valor

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Lignin, the second most abundant natural polymer, has emerged as a potential alternative material to petroleum-based chemicals and renewable resource for the production of diverse forms of aromatics, biofuels, and biobased materials. Thus, it is becoming important to understand its structure and properties to provide key features and insights for better/efficient lignin valorisation. In this work, the physicochemical characterisation of two types of industrial (technical) lignins, namely LignoBoost lignin and alkali-treated lignin was performed. Characterisation has been conducted using Brunauer-Emmett-Teller N 2 adsorption, particle size distribution, Fourier transform infrared spectroscopy, ultraviolet-visible absorption spectroscopy, gel permeation chromatography, and thermogravimetric analysis. It was found that the pretreatment severity considerably influenced the lignin composition and functional properties. The measured physicochemical properties helped in proposing potential valorisation routes for these lignins in the context of a biorefinery, focusing on their depolymerisation and subsequent biological conversion to value-added chemicals and fuels.

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Section: Resultssupporting

confidence: 82%

Physicochemical Characterisation of Technical Lignins for Their Potential Valorisation

Abdelaziz

Hulteberg

2016

Waste Biomass Valor

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“…The adsorption data fitted well with the Langmuir isotherm. The maximum adsorptions of CBH were 7.63, 5.53, and 12.32 mg/g for the three lignin fractions, which were similar to the literature (Pareek et al, 2013;Zheng et al, 2013;Machado et al, 2015;Lu et al, 2016;Sun et al, 2016). It should be noted that the binding results could be affected by the different lignin isolation/pretreatment conditions, enzyme and test conditions (temperatures, lignin loading) used for determination.…”

Section: Cbh Adsorption To Lignins By Langmuir Equationsupporting

confidence: 83%

“…It indicated that lignin with more uniform fragment size tends to have stronger binding ability on cellulase. Previous research also reported that PDI was inversely related to the interaction of the lignin with the cellulase (Berlin et al, 2006;Pareek et al, 2013).…”

Section: Cbh Adsorption To Lignins By Langmuir Equationmentioning

confidence: 94%

Characteristics of Lignin Fractions from Dilute Acid Pretreated Switchgrass and Their Effect on Cellobiohydrolase from Trichoderma longibrachiatum

Yao

Yoo

et al. 2018

Front. Energy Res.

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To investigate the interactions between acid pretreated switchgrass lignin and cellobiohydrolase (CBH), three different lignin fractions were isolated from dilute acid pretreated switchgrass by (i) ethanol extraction, followed by (ii) dioxane/H2O extraction, and (iii) cellulase treatment, respectively. Structural properties of each lignin fraction were elucidated by GPC, 13 C-NMR, and 2D-HSQC NMR analyses. The adsorptions of CBH to the isolated lignin fractions were also studied by Langmuir adsorption isotherms. Ethanolextractable lignin fraction, mainly composed of syringyl (S) and guaiacyl (G) units, had the lowest molecular weight, while dioxane/H2O-extracted lignin fraction had the lowest S/G ratio with higher content of p-coumaric acid (pCA) unit. The residual lignin fraction after enzymatic treatment had the highest S/G ratio without hydroxyphenyl (H) unit. Strong associations were found between lignin properties such as lignin composition and S/G ratio and its non-productive enzyme adsorption factors including the maximum adsorption capacity and binding strength.

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“…Lignin acts as a physical barrier that restricts the access of hydrolytic enzymes to cellulose and hemicellulose components of the plant cell wall. Moreover, it has been shown that cellulases have a tendency to bind lignin in a nonreversible manner (48)(49)(50)(51), thus limiting their availability in solution. Thermochemical pretreatment processes that are used for removal of the lignin component before cellulose degradation tend to be expensive, slow, and relatively inefficient.…”

Section: Discussionmentioning

confidence: 99%

Toward combined delignification and saccharification of wheat straw by a laccase-containing designer cellulosome

Davidi

Moraïs

Artzi

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Efficient breakdown of lignocellulose polymers into simple molecules is a key technological bottleneck limiting the production of plant-derived biofuels and chemicals. In nature, plant biomass degradation is achieved by the action of a wide range of microbial enzymes. In aerobic microorganisms, these enzymes are secreted as discrete elements in contrast to certain anaerobic bacteria, where they are assembled into large multienzyme complexes termed cellulosomes. These complexes allow for very efficient hydrolysis of cellulose and hemicellulose due to the spatial proximity of synergistically acting enzymes and to the limited diffusion of the enzymes and their products. Recently, designer cellulosomes have been developed to incorporate foreign enzymatic activities in cellulosomes so as to enhance lignocellulose hydrolysis further. In this study, we complemented a cellulosome active on cellulose and hemicellulose by addition of an enzyme active on lignin. To do so, we designed a dockerin-fused variant of a recently characterized laccase from the aerobic bacterium Thermobifida fusca. The resultant chimera exhibited activity levels similar to the wild-type enzyme and properly integrated into the designer cellulosome. The resulting complex yielded a twofold increase in the amount of reducing sugars released from wheat straw compared with the same system lacking the laccase. The unorthodox use of aerobic enzymes in designer cellulosome machinery effects simultaneous degradation of the three major components of the plant cell wall (cellulose, hemicellulose, and lignin), paving the way for more efficient lignocellulose conversion into soluble sugars en route to alternative fuels production.

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Adsorption of proteins involved in hydrolysis of lignocellulose on lignins and hemicelluloses

Cited by 131 publications

References 32 publications

Physicochemical Characterisation of Technical Lignins for Their Potential Valorisation

Physicochemical Characterisation of Technical Lignins for Their Potential Valorisation

Characteristics of Lignin Fractions from Dilute Acid Pretreated Switchgrass and Their Effect on Cellobiohydrolase from Trichoderma longibrachiatum

Toward combined delignification and saccharification of wheat straw by a laccase-containing designer cellulosome

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