Adsorption of protein on non-stoichiometric calcium—strontium hydroxyapatite

Kandori, Kazuhiko; Saito, Miwa; Saitô, Hiroshi; Yasukawa, Akemi; Ishikawa, Tatsuo

doi:10.1016/0927-7757(94)02969-5

Cited by 62 publications

(58 citation statements)

References 8 publications

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“…Massive cell apoptosis found on the FM-HA coating may be attributed to the coating barely meeting this requirement. In addition, lower compactness could result in the medium seeping deep into the coating, so that dissolution of magnesium ions and rapid increase in medium pH occurred [20,21]. During the experiment, the pH value in the cell culture medium of the FM-HA coating increased rapidly during cell growth, resulting in poor growth conditions.…”

Section: Discussionmentioning

confidence: 99%

Biocompatibility of nano-hydroxyapatite/Mg-Zn-Ca alloy composite scaffolds to human umbilical cord mesenchymal stem cells from Wharton’s jelly in vitro

Guan

Shan

Shi

et al. 2014

Sci. China Life Sci.

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Seeding cells and scaffolds play pivotal roles in bone tissue engineering and regenerative medicine. Wharton's jelly-derived mesenchymal stem cells (WJCs) from human umbilical cord represent attractive and promising seeding cells in tissue regeneration and engineering for treatment applications. This study was carried out to explore the biocompatibility of scaffolds to seeding cells in vitro. Rod-like nano-hydroxyapatite (RN-HA) and flake-like micro-hydroxyapatite (FM-HA) coatings were prepared on Mg-Zn-Ca alloy substrates using micro-arc oxidation and electrochemical deposition. WJCs were utilized to investigate the cellular biocompatibility of Mg-Zn-Ca alloys after different surface modifications by observing the cell adhesion, morphology, proliferation, and osteoblastic differentiation. The in vitro results indicated that the RN-HA coating group was more suitable for cell proliferation and cell osteoblastic differentiation than the FM-HA group, demonstrating better biocompatibility. Our results suggested that the RN-HA coating on Mg-Zn-Ca alloy substrates might be of great potential in bone tissue engineering.nano-hydroxyapatite, Mg-Zn-Ca alloy, mesenchymal stem cells, Wharton's jelly, biocompatibility Citation:Guan FX, Ma SS, Shi XY, Ma X, Chi LK, Liang S, Cui YB, Wang ZB, Yao N, Guan SK, Yang B. Biocompatibility of nano-hydroxyapatite/ Mg-Zn-Ca alloy composite scaffolds to human umbilical cord mesenchymal stem cells from Wharton's jelly in vitro.

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Section: Discussionmentioning

confidence: 99%

Biocompatibility of nano-hydroxyapatite/Mg-Zn-Ca alloy composite scaffolds to human umbilical cord mesenchymal stem cells from Wharton’s jelly in vitro

Guan

Shan

Shi

et al. 2014

Sci. China Life Sci.

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“…However, in contrast, the amount of LSZ adsorbed on Hap particles at saturation ( ) decreased as the Ca/P ratio increased and approached zero at ca. Ca/P = 1.70 (Kandori et al 2000). No relationship with the Ca/P ratio was detected for the neutral protein MGB (Kandori et al 2000).…”

Section: Introductionmentioning

confidence: 89%

“…Ca/P = 1.70 (Kandori et al 2000). No relationship with the Ca/P ratio was detected for the neutral protein MGB (Kandori et al 2000).…”

Section: Introductionmentioning

confidence: 89%

“…In this respect, many important studies have been reported to date (Kawasaki et al 1986b;Tiselius et al 1956; Thomann et al 1989;Gorbunoff 1984;John and Schmidt 1984). Indeed, the present authors have undertaken fundamental studies of the adsorptions of acidic BSA, neutral MGB and basic LSZ onto various kinds of synthetic Hap particles during the past decade (Kandori et al 1992(Kandori et al , 1995a(Kandori et al -c, 2000(Kandori et al , 2002. These studies have shown that the amount of BSA adsorbed at saturation, , depends on the Ca/P molar ratio in the materials employed, with the value of increasing as the Ca/P ratio increased.…”

Section: Introductionmentioning

confidence: 99%

“…Since the space group of crystalline calcium hydroxyapatite [Ca 10 (PO 4 ) 6 (OH) 2 , Hap] is P6 3 /m and its unit cell parameters are a = b = 0.943 nm and c = 0.688 nm associated with two different binding sites (called C and P sites) on the particle surface (Kawasaki et al 1985(Kawasaki et al , 1986a, it exhibits multiple-site binding characteristics towards proteins. When Hap particles are dispersed in an aqueous medium, calcium atoms (C sites) are exposed on the Hap surface by dissolution of OH − ions at the particle surface.…”

Section: Introductionmentioning

confidence: 99%

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Thermodynamic Study of Protein Adsorption onto Synthetic Calcium Hydroxyapatites

Kandori

Takaragi

Murata

et al. 2005

Adsorption Science & Technology

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Thermodynamic parameters have obtained in the present study by measuring the adsorption isotherms of bovine serum albumin (BSA: isoelectric point (iep) = 4.7), myoglobin (MGB: iep = 7.0) and lysozyme (LSZ: iep = 11.1) onto calcium hydroxyapatite (Hap) at different temperatures (15°C and 35°C) in an attempt to clarify the adsorption mechanism of such proteins. The adsorption affinity of proteins strongly depended on the Ca/P molar ratio of the Hap particles. In the case of BSA, the initial slopes of the adsorption isotherms increased remarkably with an increase in the Ca/P ratio. In contrast, the initial slopes of the adsorption isotherms for LSZ changed gradually with increasing Ca/P ratio. Finally, no Ca/P ratio dependence was observed in the MGB system. These results were explained in terms of the electrostatic interaction between the proteins and Hap. The negative values of ∆G ads for the BSA system increased from -34.0 kJ/mol to -36.1 kJ/mol with increasing Ca/P ratio whereas those for the LSZ system decreased from -37.9 kJ/mol to -30.8 kJ/mol. A diminished dependence of ∆G ads on the Ca/P ratio was observed in the MGB system. The adsorptions of LSZ and MGB onto Hap were exothermic which again supports an electrostatic attraction mechanism. In contrast, the adsorption of BSA onto Hap exhibited a large positive value for ∆H ads , indicating that the process was endothermic. The ∆S ads value of the LSZ system was negative while those for the BSA and MGB systems were positive. Positive values for ∆S ads , especially for the BSA system, indicate that the adsorption processes for BSA and MGB (both with a lower structural stability) were driven by an entropy gain, i.e. ∆S ads > 0. Adsorption of these proteins onto the Hap surface was accompanied by a structural rearrangement.

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Effect of albumin on brushite transformation to hydroxyapatite

Xie

Riley

Chittur

2001

J. Biomed. Mater. Res.

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Brushite (CaHPO(4) x 2H(2)O) is a precursor to hydroxyapatite [HA, Ca(5)(PO(4))(3)OH]. It has been shown that a modified form of brushite, with potassium substituting for calcium at specific sites, demonstrated accelerated transformation to HA when exposed to nonproteinaceous Hanks' balanced aqueous salt solutions (HBSS). The biocompatibility of a transforming material is related to cellular response to the process, which is initiated by protein adsorption. The effect of adsorbed protein on the kinetics and chemistry of brushite transformation to HA, when exposed to HBSS containing bovine serum albumin (BSA), was examined using Fourier transform IR spectroscopy, X-ray diffraction, and energy dispersive spectrometry techniques. The effect of solution pH was also studied. Results show that, in the presence of a protein-free environment, transformation is faster in buffered medium than in nonbuffered medium. Moreover, curve fitting and second derivatives of the IR spectra show that some bands shift depending on whether the brushite transforms in a buffered or nonbuffered medium. Therefore, variation in pH affects both transformation rate and the associated chemistry. The presence of BSA in either buffered or nonbuffered medium retards the transformation in comparison to the corresponding BSA-free medium. The extent of this retardation increases with the increase in bulk concentration of BSA but does not alter the transformation chemistry. This suggests the retardation on the transformation rate is due to BSA adsorption coverage on the calcium phosphate ceramic. This may be due to the shielding of Ca(2+) and PO(4)(-3) sites, preventing their interaction with the HBSS.

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Adsorption of protein on non-stoichiometric calcium—strontium hydroxyapatite

Cited by 62 publications

References 8 publications

Biocompatibility of nano-hydroxyapatite/Mg-Zn-Ca alloy composite scaffolds to human umbilical cord mesenchymal stem cells from Wharton’s jelly in vitro

Biocompatibility of nano-hydroxyapatite/Mg-Zn-Ca alloy composite scaffolds to human umbilical cord mesenchymal stem cells from Wharton’s jelly in vitro

Thermodynamic Study of Protein Adsorption onto Synthetic Calcium Hydroxyapatites

Effect of albumin on brushite transformation to hydroxyapatite

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