Adsorption of peroxidase on titanium surfaces: A pilot study

Abstract

The present study demonstrates the in vitro and in vivo adsorption of peroxidase onto titanium surfaces. Titanium foils (mean ± SEM: 365 ± 2 mm2, n = 114) were incubated during 30 min with lactoperoxidase (4 mg in 5 mL 100 mM phosphate buffer pH 7). After 15 washings by H2O, titanium foils were incubated with o‐phenylenediamine (6 mg/mL) and H2O2 (7 mM) during 30 min. The reaction was then stopped by the addition of HCI 1M and the absorbance of the liquid phase was read on a spectrophotometer at 492 nm. In vitro adsorbed lactoperoxidase onto titanium surfaces was 0.70 ± 0.05 ng/mm2 (mean ± SEM, n = 30). X‐ray photoelectron spectroscopy confirmed the incorporation of protein nitrogen onto titanium surfaces: the nitrogen atomic percentage increased from 0.9 ± 0.3 to 12.7 ± 0.2% (n = 3) and from 3.7 ± 0.1 to 14.4 ± 0.4% (n = 5) when titanium foils were incubated in the lactoperoxidase solution during 30 min and 24 h respectively. In vivo, oral peroxidases adsorbed on titanium healing abutments from 0.01 to 0.58 ng/mm2 (n = 19) after 2 weeks in the oral environment. © 2000 John Wiley & Sons, Inc. J Biomed Mater Res, 52, 567–571, 2000.

The Peri‐Implantitis: Implant Surfaces, Microstructure, and Physicochemical Aspects

“…The use of hydrogen peroxide H 2 O 2 evaporated on titanium surface using CO 2 laser relates to a simple physicochemical concept. H 2 O 2 is quickly adsorbed on a titanium implant surface 67,68 . The use of a powerful oxidizer such as H 2 O 2 and the laser‐induced heat makes it possible to accelerate considerably the exchanges in titanium and oxygen atoms through the surface layer of titanium oxide.…”

Candida albicans biofilm on titanium: effect of peroxidase precoating

“…Titanium pieces were cut from native foil (Advent ® , Oxford; 99.6% purity; thickness 25 µm, size 25 mm × 5 mm). As previously described, 13 each piece was weighed and the total surface was calculated using the following algorithm: S = 2 {(m/ρh) + h(L + I)} where S = total surface (mm 2 ), m = foil mass (mg), ρ = volumic mass (4.544 mg/mm 3 ), h = foil thickness (0.025 mm), L = foil length (mm), l = foil width (mm). Total surface averaged 246 ± 4 mm² (n = 16) with a variation coefficient of 6.5%.…”

“…Adsorption of lactoperoxidase (LP) onto titanium surfaces, which has antimicrobial properties similar to those of oral peroxidase, has already been described. 13 LP produces hypothiocyanite (OSCN -) and hypothiocyanous acid (HOSCN) compounds from thiocyanate (SCN -) in the presence of hydrogen peroxide (H 2 O 2 ). In vitro, iodide (I -) has also been used as substrate, because it is oxidized into hypoiodite (OI -) or hypoiodous acid (OIH) by peroxidase activity.…”

<title>New microchannel device for protein separation and detection</title>

“…In addition, the intensity of total nitrogen peak with protein is much larger than that with buffer, which is due to the large amount of nitrogen in the protein structure [10]. In addition, the intensity of total nitrogen peak with protein is much larger than that with buffer, which is due to the large amount of nitrogen in the protein structure [10].…”