Adsorption of immunoglobulin Y in supermacroporous continuous cryogel with immobilized Cu2+ ions

Júnior, Willer Ferreira da Silva; Cano, Rodrigo; Tótola, Antônio Helvécio; Carvalho, L. M.; Cerri, Marcel O.; Coimbra, Jane Sélia dos Reis; Carvalho, Gleidson Giordano Pinto de; Carvalho, Bruna Mara Aparecida de

doi:10.1016/j.chroma.2015.03.052

Cited by 19 publications

(1 citation statement)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cu 2+ -affinity cryogels for absorption protein have been thoroughly investigated. Júnior et al have studied the Cu 2+ –IDA affinity cryogel-adsorbing immunoglobulin Y (IgY) and provided useful information of the maximum adsorption yield and the dissociation constants of IgY that was used in the simulation of purification process of immobilized metal affinity chromatography (IMAC). Evli et al immobilized amyloglucosidase onto the Cu 2+ –IDA affinity cryogels for the hydrolysis of starch.…”

Section: Introductionmentioning

confidence: 99%

Preparation and Properties of Cryogel Based on Poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate)

Wan

et al. 2019

Langmuir

View full text Add to dashboard Cite

The immobilized metal affinity cryogels based on poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (p(HEMA-GMA)) containing hydroxy and epoxy groups were prepared by free-radical copolymerization under cryogenic condition and then functionalized with iminodiacetic acid and chelated Cu2+, Ca2+, and Fe3+ ions to the p(HEMA-GMA) cryogel. The structures of p(HEMA-GMA) and immobilized metal-affinity cryogels were analyzed by Fourier transform infrared spectroscopy and scanning electron microscopy (SEM)–energy dispersive X-ray spectroscopy. SEM results showed that the prepared cryogels had interconnected pores with the size of 30–100 μm. The performance of water swelling into the cryogels was fitted in Fickian diffusion. The adsorption property of cryogels was influenced by the immobilized ionic type, temperature, and adsorbate. The adsorption capacity of immobilized Cu2+ cryogel (p(HEMA)–Cu2+ (0.5 M) cryogel) was the highest in comparison with that of Ca2+ and Fe3+ affinity cryogels under the same condition. The maximum adsorption capacity of p(HEMA)–Cu2+ (0.5 M) cryogel for porcine pancreatic lipase was 150.14 mg/g at a higher temperature of 35 °C, whereas for bovine serum albumin, the maximum adsorption capacity was 154.11 mg/g at a lower temperature of 25 °C. The research of thermodynamics and kinetics indicated that the mechanism of the protein adsorption process corresponded to the Langmuir model and pseudo-second-order model.

show abstract

Section: Introductionmentioning

confidence: 99%

Preparation and Properties of Cryogel Based on Poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate)

Wan

et al. 2019

Langmuir

View full text Add to dashboard Cite

show abstract

Molecularly imprinted polymeric shell coated monodisperse‐porous silica microspheres as a stationary phase for microfluidic boronate affinity chromatography

Süngü

Kip

Tuncel

2019

J of Separation Science

View full text Add to dashboard Cite

Molecular imprinting of cis-diol functionalized agents via boronate affinity interaction has been usually performed using nanoparticles as a support which cannot be utilized as a stationary phase in continuous microcolumn applications. In this study, monodisperse-porous, spherical silica particles in the micron-size range, with bimodal pore diameter distribution were selected as a new support for the synthesis of a molecularly imprinted boronate affinity sorbent, using a cis-diol functionalized agent as the template. A specific surface area of 158 m 2 /g was achieved with the imprinted sorbent by using monodisperse-porous silica microspheres containing both mesoporous and macroporous compartments as the support. High porosity originating from the macroporous compartment and sufficiently high particle size provided good column permeability to the imprinted sorbent in microcolumn applications. The mesoporous compartment provided a large surface area for the parking of imprinted molecules while the macroporous compartment facilitated the intraparticular diffusion of imprinted target within the microsphere interior. A microfluidic boronate affinity system was first constructed by using molecularly imprinted polymeric shell coated monodisperse-porous silica microspheres as a stationary phase. The synthetic route for the imprinting process, the reversible adsorption/ desorption behavior of selected target and the selectivity of imprinted sorbent in both batch and microfluidic boronate affinity chromatography systems are reported. K E Y W O R D Sadsorption, microfluidic systems, molecular imprinting, nucleotides, solid-phase extraction Article Related Abbreviations: EDMA, ethylene dimethacrylate; EDX, Energy Dispersive X-Ray Spectroscopy; HEPES, hydroxyethylpiperazine ethanesulfonic acid; IF, Imprinting factor; MAA, methacrylic acid; MIPS@SiO 2 microspheres, molecularly imprinted polymeric shell coated silica microspheres, by using β-NAD as the template; NIPS@SiO 2 microspheres, non-imprinted polymeric shell coated silica microspheres; β-NAD, β-nicotinamide adenine dinucleotide; SSA, specific surface area; TMSPM, 3-trimethoxysilylpropyl methacrylate; VPBA, 4-vinylphenylboronic acid.

show abstract

Extraction and Purification of IgY

Morgan

Freire

Tavares

et al. 2021

IgY-Technology: Production and Application of Egg Yolk Antibodies

View full text Add to dashboard Cite

Adsorption of immunoglobulin Y in supermacroporous continuous cryogel with immobilized Cu2+ ions

Cited by 19 publications

References 38 publications

Preparation and Properties of Cryogel Based on Poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate)

Preparation and Properties of Cryogel Based on Poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate)

Molecularly imprinted polymeric shell coated monodisperse‐porous silica microspheres as a stationary phase for microfluidic boronate affinity chromatography

Extraction and Purification of IgY

Contact Info

Product

Resources

About