Adsorption of double-stranded DNA to graphene oxide preventing enzymatic digestion

Lei, Haozhi; Mi, Lijuan; Zhou, Xuejiao; Chen, Jiajia; Hu, Jun; Guo, Shouwu; Zhang, Yi

doi:10.1039/c1nr10617a

Cited by 116 publications

(114 citation statements)

References 60 publications

(62 reference statements)

Supporting

Mentioning

108

Contrasting

Order By: Relevance

“…However, this protective effect seems more controversial and subject to debate in the case of double stranded NA. Lei et al demonstrated that this protective effect was highly dependent on salt concentrations in the case of double stranded NA and that it could be reversed by the addition of an anionic surfactant such as triton X-100 23 . In addition, enzymatic digestion by DNAse I and EcoR I has been shown to occur even if double stranded DNA was partially adsorbed onto GO whereas resistance to degradation by Exo III was reported at the same time 21 .…”

Section: What Can Gbms Offer As Gene Delivery Platforms?mentioning

confidence: 99%

Graphene materials as 2D non-viral gene transfer vector platforms

2016

View full text Add to dashboard Cite

Advances in genomics and gene therapy could offer solutions to many diseases that remain incurable today, however one of the critical reasons halting clinical progress is due to the difficulty in designing efficient and safe delivery vectors for the appropriate genetic cargo. Safety and largescale production concerns counter-balance the high gene transfer efficiency achieved with viral vectors, while non-viral strategies have yet to become sufficiently efficient. The extraordinary physicochemical, optical and photothermal properties of graphene-based materials (GBMs) could offer two-dimensional (2D) components for the design of nucleic acid carrier systems. We discuss here such properties and their implications for the optimization of gene delivery. While the design of such vectors is still in its infancy, we provide here an exhaustive and up-to-date analysis of the studies that have explored GBMs as gene transfer vectors, focusing on the functionalization strategies followed to improve vector performance and on the biological effects attained.

show abstract

Section: What Can Gbms Offer As Gene Delivery Platforms?mentioning

confidence: 99%

Graphene materials as 2D non-viral gene transfer vector platforms

2016

View full text Add to dashboard Cite

show abstract

“…7,8 It is now generally accepted that the in vitro cellular toxicity of graphene is closely related to its surface functionalization. Moreover, the reactive groups on graphene surface may facilitate conjugation with various systems, such as polymers, 9 biomolecules, 10 DNA, 11 protein, 12 quantum dots, 13 and others, imparting GO with multifunctionalities for diverse biological and medical applications.…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo effects of graphene oxide and reduced graphene oxide on glioblastoma

Jaworski¹,

Sawosz²,

Kutwin³

et al. 2015

IJN

View full text Add to dashboard Cite

Graphene and its related counterparts are considered the future of advanced nanomaterials owing to their exemplary properties. However, information about their toxicity and biocompatibility is limited. The objective of this study is to evaluate the toxicity of graphene oxide (GO) and reduced graphene oxide (rGO) platelets, using U87 and U118 glioma cell lines for an in vitro model and U87 tumors cultured on chicken embryo chorioallantoic membrane for an in vivo model. The in vitro investigation consisted of structural analysis of GO and rGO platelets using transmission elec tron microscopy, evaluation of cell morphology and ultrastructure, assessment of cell viability by XTT assay, and investigation of cell proliferation by BrdU assay. Toxicity in U87 glioma tumors was evaluated by calculation of weight and volume of tumors and analyses of ultrastructure, histology, and protein expression. The in vitro results indicate that GO and rGO enter glioma cells and have different cytotoxicity. Both types of platelets reduced cell viability and proliferation with increasing doses, but rGO was more toxic than GO. The mass and volume of tumors were reduced in vivo after injection of GO and rGO. Moreover, the level of apoptotic markers increased in rGO-treated tumors. We show that rGO induces cell death mostly through apoptosis, indicating the potential applicability of graphene in cancer therapy.

show abstract

“…Researches about the effects of graphene sheets and/ or GO on macro-biomolecules, such as DNA molecules [16][17][18], gene [19], and enzymes [20], have caught great interests, where results have shown several potential applications in bioassay and bioanalysis.…”

Section: Biomolecular Interfacingmentioning

confidence: 99%

“…Since GO has a high affinity to ssDNA, it has been used as promising sequential delivery vehicle [22], geneprotecting [16], and transfection agent [23]. Y. Zhang et al have investigated the π-π interaction between dsDNA and GO in buffer with different metal ions.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Biological Applications of Graphene and Graphene Oxide

Wu¹,

Zhang²,

Wu³

et al. 2012

Nano BioMed ENG

Self Cite

View full text Add to dashboard Cite

Graphene, as a steady two dimensional (2D) carbon material, possesses intriguing physical and chemical properties, which arouses great interests of scientists for its applications in enormous fields. In particular, graphene and graphene oxide have been widely used for drug delivery and DNA detection based on π-π stacking and hydrophobic interactions. Besides, graphene with fluorescent molecules or nanoparticles and graphene quantum dots have also been frequently applied as fluorescent probe. In this article, advances of graphene and graphene oxide on biomedical applications will be highlighted from the perspective of biomolecular interaction, cell imaging, drug delivery, and toxicity.

show abstract

Adsorption of double-stranded DNA to graphene oxide preventing enzymatic digestion

Cited by 116 publications

References 60 publications

Graphene materials as 2D non-viral gene transfer vector platforms

Graphene materials as 2D non-viral gene transfer vector platforms

In vitro and in vivo effects of graphene oxide and reduced graphene oxide on glioblastoma

Biological Applications of Graphene and Graphene Oxide

Contact Info

Product

Resources

About