Adsorption of chemically synthesized mussel adhesive peptide sequences containing DOPA on stainless steel

Abstract

The adsorption of proteins at solid-liquid interfaces is important in biosensor and biomaterial applications. Marine mussels affix themselves to surfaces using a highly cross-linked, protein-based adhesive containing a high proportion of L-3,4-dihydroxyphenylalanine (DOPA) residues. In this work, the effect of DOPA residues on protein adhesion on stainless steel surfaces was studied using a quartz crystal microbalance with dissipation system. The adsorption of two repetitive peptide motifs, KGYKYYGGSS and KGYKYY, from the mussel Mytilus edulis foot protein 5 on stainless steel was studied before and after chemo-enzymatic modification of tyrosine residues to DOPA using mushroom tyrosinase. Conversion from tyrosine to DOPA, evaluated by HPLC, was in the range 70-99%. DOPA-modified sequences showed fourfold greater adhesion than unmodified M. edulis foot protein 5 motifs.

Endothelialization of Titanium Surfaces by Bioinspired Cell Adhesion Peptide Coatings

“…As a result, nitinol stents, which are frequently used due to their unique mechanical properties and excellent biocompatibility, in fact display a TiO 2 surface that enables an easy functionalization with DOPA-containing peptides by dip coating. This mechanism is transferable to stainless steel, which was likewise coatable with the mussel-derived peptide and provides an alternative to nitinol when higher mechanical stability is desired. Herein, a coordination bond between the catechol and the respective metal oxide is formed .…”

Adsorption and Orientation of 3,4-Dihydroxy-l-phenylalanine onto Tunable Monolayer Films

“…The adhesion proteins secreted by marine mussels bind strongly to virtually all inorganic and organic surfaces in aqueous environments, accordingly serving as an intractable problem in the field of marine antifouling. , A common feature of such proteins is that they contain a high content of 3,4-dihydroxy- l -phenylalanine ( l -DOPA), which is considered to be responsible for their capacity to compete successfully with water at the surface and cross-link under water. , In the most recent decade, interaction mechanisms of DOPA side chains embedded in proteins and solid surfaces have been extensively studied, owing to a growing attraction in the respects of antifouling strategies and biomimetic adhesion. − It can be concluded that DOPA plays an important part in the interactions through H-bonding, , coordination with metal/metal oxide, , or covalent cross-linking, , of which the detailed binding mechanisms of DOPA to different surfaces is an extremely important research topic, attracting great interests of scientists.…”

Design, Preparation, and Application of a Novel, Microencapsulated, Intumescent, Flame-Retardant-Based Mimicking Mussel

“…13−16 Many research studies are focused on revealing and mimicking the adhesive materials. The mimicking mussel is widely used in medicine, food, metallurgy, and other fields, 17−19 whose application in the surface modification of the flame retardant has not been reported. Tannic acid is a complex polyhydric phenol, in which there are multiple phenolic hydroxyl groups, which can be a carbonization agent in IFR and simultaneously form a stable chelate with Fe 3+ .…”

“…Mussels are common creatures living in the ocean, which can secrete strong adhesion protein, clinging to the surface of many substances such as bone grafts, plants, glass products, metal components, polymer materials, minerals, and so forth. − Many research studies are focused on revealing and mimicking the adhesive materials. The mimicking mussel is widely used in medicine, food, metallurgy, and other fields, − whose application in the surface modification of the flame retardant has not been reported. Tannic acid is a complex polyhydric phenol, in which there are multiple phenolic hydroxyl groups, which can be a carbonization agent in IFR and simultaneously form a stable chelate with Fe 3+ .…”