Adsorption of cell-penetrating peptide Tat2 and polycation luviquat FC-370 to triticale microspore exine

Self-aggregation propensity of the Tat peptide revealed by UV-Vis, NMR and MD analyses

“…For instance, it was reported that dimers of the Tat peptide, obtained by covalent bonding or lysine linkage at the C-termini, show enhanced cellular activity in terms of uptake efficacy. [18][19][20][21][22] In addition, we also recently demonstrated that peptide self-aggregation into stable nanoparticles emerges as a critical physicochemical property of short amphiphilic peptides. 23 Inspired by the latter preliminary pieces of evidence, here we have set out to probe for the first time the spontaneous propensity of the Tat peptide to form aggregates as a function of concentration by using a straightforward combination of in cuvette UV-Vis-based analyses of peptide physico-chemical properties, NMR-based analysis of peptide diffusion, and Molecular Dynamics (MD) simulations.…”

Cellular Delivery of Plasmid DNA into Wheat Microspores Using Rosette Nanotubes

“… 23 Microspores, which are immature pollen grains, have great potential due to their ability to produce mature double haploid plants from single cells under the right treatment conditions, usually involving stress. 24 However, the main hindrance to transfection and reproducibility is the presence of an impervious microspore exine and intine, which limits entry to the cell. The delivery is presumed to occur through the micropore, which is a small opening in the exine.…”

“…This process allows the transfection of the plant microspores with the genetic materials in vivo and the immature transfected microspores are then isolated and cultured to produce a transgenic doubled haploid plant . Microspores, which are immature pollen grains, have great potential due to their ability to produce mature double haploid plants from single cells under the right treatment conditions, usually involving stress . However, the main hindrance to transfection and reproducibility is the presence of an impervious microspore exine and intine, which limits entry to the cell.…”

“…The delivery is presumed to occur through the micropore, which is a small opening in the exine. Nevertheless, it has been possible for CPPs to penetrate viable microspores and deliver protein. , Out of all of these nanomaterials, carbon nanotubes have so far generated the most impact. Cationic polymers complexed with single-walled carbon nanotubes were successfully used to deliver genetic materials in mature plants through infiltration and into protoplasts. , This subsequently led to a trail of interesting works.…”

“…Nevertheless, it has been possible for CPPs to penetrate viable microspores and deliver protein. 24,25 Out of all of these nanomaterials, carbon nanotubes have so far generated the most impact.…”