Adsorption of bovine serum albumin and urease by biochar

Wang, Wenjing; Chen, Lei; Zhang, Yipeng; Liu, Guocheng

doi:10.1088/1755-1315/61/1/012143

Cited by 3 publications

(3 citation statements)

References 13 publications

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“…In contrast, the N 2 -based specific surface area and total pore volume decreased slightly, possibly because of the adsorption of components from the DMEM. This observation is consistent with the adsorption of bovine serum albumin previously described by Wang et al 16 Nevertheless, because only a relatively small proportion of the total DMEM in the experiment was adsorbed, the sorption of media components cannot explain the observed cytotoxic effects and can have had an only minor effect (if any) on PAH desorption due to pore blockage.…”

Section: Resultssupporting

confidence: 92%

Cytotoxicity of Biochar: A Workplace Safety Concern?

Sigmund

Huber

Bucheli

et al. 2017

Environ. Sci. Technol. Lett.

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Biochar has a number of environmental applications, including soil amendment for agriculture, remediation of contaminated soils and sediments, and climate change mitigation. Dust formed during its production and field application may pose a health risk, but the cytotoxicity of biochar has, to the best of our knowledge, not previously been investigated. Therefore, we measured the concentration-dependent cytotoxicity of biochar on an NIH 3T3 mouse fibroblast cell line. We used a contaminant trap experiment to measure the total and nondesorbable polycyclic aromatic hydrocarbon (PAH) fractions of the biochar. PAH release was found to be negligible because of the biochar’s strong PAH sorption potential. The biochar was nevertheless observed to have a cytotoxic effect on the fibroblast cells; the EC10 values were 49.6 and 18.8 μg/mL after incubation for 24 and 48 h, respectively. This cytotoxic effect is likely to relate to the particulate nature and size distribution of the biochar; the biochar had particles similar in size to atmospheric particulate matter (PM2.5) that bound to the fibroblast cell surface. To minimize the risk of exposure, practitioners should wear respiratory protective equipment during biochar production. During field application, biochar should be applied in slurries and should always be mixed with a soil matrix to avoid secondary dust formation.

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Section: Resultssupporting

confidence: 92%

Cytotoxicity of Biochar: A Workplace Safety Concern?

Sigmund

Huber

Bucheli

et al. 2017

Environ. Sci. Technol. Lett.

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“…The differences in surface coverages combined with the equal amounts of adsorbed proteins indicate that most proteins in the S. pasteurii total protein extract have a higher affinity towards hydrophobic surfaces. In fact, several studies have shown that most proteins have a high affinity towards hydrophobic surfaces, including hydrophilic proteins such as BSA, with increased amounts of adsorbed proteins displayed on surfaces with higher hydrophobicity levels [20][21][22][23][24][25][26]55]. Furthermore, though hydrophobic amino acid residues are mostly ingrained inside the protein's molecular structure, a hydrophobic protein core can become exposed due to structural unfolding upon initial binding via electrostatic interactions.…”

Section: Protein Adsorptionmentioning

confidence: 99%

“…In particular, protein adsorption is highly dependent on the surface chemistries of the protein and the sorbent surface, as well as the presence of other proteins. For example, proteins commonly exhibit high affinity towards hydrophobic surfaces, even if they are hydrophilic in nature [20][21][22][23][24][25][26], but that often leads to protein denaturation and sharp decreases in enzymatic activity [27]. Although soils generally display low levels of hydrophobicity [28,29], hydrophobic soils, defined as having contact angles ≥ 90 • , have been documented worldwide and are especially common in surface soils that often dry out or have been exposed to fires [28,[30][31][32][33][34][35][36].…”

Section: Introductionmentioning

confidence: 99%

Effects of Soil Surface Chemistry on Adsorption and Activity of Urease from a Crude Protein Extract: Implications for Biocementation Applications

Vilar

Ikuma

2022

Catalysts

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In the bacterial enzyme-induced calcite precipitation (BEICP) technique for biocementation, the spatial distribution of adsorbed and catalytically active urease dictates the location where calcium carbonate precipitation and resulting cementation will occur. This study investigated the relationships between the amount of urease and total bacterial proteins adsorbed, the retained enzymatic activity of adsorbed urease, and the overall loss of activity upon adsorption, and how these relationships are influenced by changes in soil surface chemistry. In soils with hydrophobic contents higher than 20% (w/w) ratio, urease was preferentially adsorbed compared to the total amount of proteins present in the crude bacterial protein extract. Conversely, adsorption of urease onto silica sand and soil mixtures, including iron-coated sand, was much lower compared to the total proteins. Higher levels of urease activity were retained in hydrophobic-containing samples, with urease activity decreasing with lower hydrophobic content. These observations suggest that the surface manipulation of soils, such as treatments to add hydrophobicity to soil surfaces, can potentially be used to increase the activity of adsorbed urease to improve biocementation outcomes.

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Adsorption of Organic Pollutants on Carbonaceous Adsorbents Prepared by Direct Activation of Sweet Cherry Stones

Nowicki,

Kaźmierczak,

Wiśniewska

2024

ChemPhysChem

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The main objective of this study was to assess the usefulness of the sweet cherry stones for the production of carbonaceous adsorbents by means of direct physical activation method, using conventional and microwave variant of heating. The adsorbents were characterized in terms of textural parameters, acidic‐basic character of the surface, electrokinetic properties and their suitability for drinking water purification. Adsorption tests were carried out against three organic compounds: Triton X‐100 (surfactant), bovine serum albumin (protein) and methylene blue (synthetic dye). Depending on the variant of heating applied during activation procedure, the obtained activated biochars differed significantly in terms of the elemental composition, acidic‐basic properties as well as degree of specific surface development and the type of porous structure generated. Adsorption tests have showed that the efficiency of organic pollutants removal from aqueous solutions depends significantly not only on the type of the adsorbent and adsorbate applied, but also on the temperature and pH of the system. The sample prepared by microwave‐assisted direct activation proved to be very effective in terms of all tested organic pollutants adsorption. The maximum sorption capacity toward Triton‐100, bovine serum albumin and methylene blue reached the level of 86.5, 23.4 and 81.1 mg/g, respectively.

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Adsorption of bovine serum albumin and urease by biochar

Cited by 3 publications

References 13 publications

Cytotoxicity of Biochar: A Workplace Safety Concern?

Cytotoxicity of Biochar: A Workplace Safety Concern?

Effects of Soil Surface Chemistry on Adsorption and Activity of Urease from a Crude Protein Extract: Implications for Biocementation Applications

Adsorption of Organic Pollutants on Carbonaceous Adsorbents Prepared by Direct Activation of Sweet Cherry Stones

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